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Flashcards in NLS Deck (8)
1

NLS

Nuclear localization sequence

2

NLS characteristics

not necessarily N-terminus; basic; not cleaved; importins and GTPase

3

a,b importins

responsible for recognizing NLS and translocating it to the nucleus

4

Ran GTP

responsible for removing a,b importins

5

CAS

GTPase activity; hydrolyses ran GTP and causes its translocation out of the nucleus

6

NTF2

responsible for bringing ran-gdp into the nucleus

7

ran-gef

phosphorylates Ran-GDP

8

NLS mode of action

1) a,b importins bind to NLS of protein and translocate it into the nucleus 2) Ran-GTP binds to a,b importins and causes their removal 3) CAS hydrolyzes ran GTP causing its translocation out of the nucleus 4) NFT2 binds ran-GDP bringing it back into the nucleus 5) Ran-GEF causes phosphorylation of Ran-GDP

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