Enzyme Mechanisms Flashcards Preview

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Flashcards in Enzyme Mechanisms Deck (30):
1

What are the four types of enzymatic regulation?

1) Non-covalent modification (inhibitor, activator) 2) Covalent modification (inhibitor, activator) 3) Irreversible (at the protein level) 4) Reversible ( at the protein level)

2

Allostery

Non-covalent modification; binding of a ligand at one site of an enzyme causes differences in binding at different site; allosteric proteins usually have multiple subunits; allostery appears to modify Km.

3

What four amino acid commonly phosphorylated?

Ser, Thr, Tyr, His

4

What amino acid is commonly adenylated?

Tyr

5

What enzyme catalyzes the addition of a phosphate group?

Phosphorylase

6

What enzyme catalyzes the addition of AMP or an adenyl group?

Adenylase

7

Ubiquitination

The process by which the protein ubiquin is added to a compound; associated with Lys; Poly marks target for degradation; Mono- changes activity

8

What types of modifications are used to modify enzyme activity?

1) Phosphorylation, Adenylation, ubiquitination, addition of a hydrophobic group, cellular pH.

9

SCCAM

Five Mechanisms of catalysis (Strain, Covalent, Cage-effect, Acid-base, Metal ion)

10

What are the 6 enzyme classes?

HOT LILY; 312456; Hydrolase, Oxidoreductase, Transferase, Lyase, isomerase, ligase.

11

Oxidoreductase

EC: 1; Mechanism of catalysis Transfer of electrons

12

What are the three types of oxidoreductases?

1st, Oxidoreductases transfer electrons; Dehydrogenase, Oxidase, Oxygenase

13

Dehydrogenase

Oxidoreductase; Transfer of a hydrogen from donor to acceptor

14

Oxidase

Oxidoreductase; Transfer of hydrogen (or e-) from donor to oxygen

15

Oxygenase

oxidoreductase; Incorporation of oxygen into a substrate

16

Transferase

EC 2; enzyme mechanism; transfer functional groups (two types Kinase and Transaminase)

17

Kinase

Transferase; Transfer of a phosphoryl group

18

Transaminase

Transferase; Transfer of an amino group to a keto group

19

Hydrolase

EC 3; Single bond cleavage by water incorporation; two types (phosphatase, sulfatase)

20

Phosphatase

Hydrolase; Removal of phosphate groups

21

Sulfatase

Hydrolase; Removal of sulfate groups

22

Lyase

EC 4; Bond cleavage by elimination; naming (hydro-lase; thio-lyase.. etc.)

23

Isomerase

EC 5; Intramolecular group transfer; two types (mutase, epimerase)

24

Mutase

Isomerase; intramolecular group transfer

25

Epimerase

Isomerase; conversion of chirality at one carbon atom

26

Ligase

EC 6; Joining two molecules; ATP dependent

27

Synthetase

Ligase; A common name for ligase.

28

Nucleophile

a compound with a strong tendance to donate electrons

29

Electrophile

a compound with a strong tendency to accept electrons

30

Hexokinase

look at the suffix!! It is a kinase. A kinase is a transferase, this works in glycolysis; uses the cage effect and induced fit

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