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Flashcards in Energy metabolism Deck (43)
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0

What would upregulate the TCA cycle?

NAD+

1

What is the toxic effect of CN-?

binds tightly to heme iron in cytochrome oxidase which oxidative phosphorylation

2

What is your anaerobic fate of pyruvate?

anaerobically, there is no TCA cycle to burn up the reduced NADH

must recycle NADH --> NAD+ otherwise glycolysis will stop

Electrons are a waste product, and they must be disposed of on something other than oxygen

3

What is the aerobic fate of pyruvate?

electrons are source of Energy

4

What is delta G?

change in free energy

5

delta G nott

at standard concentration conditions

6

delta G nott '

change in Gibbs free energy @ 25 C and pH 7

7

with regards to free energy, in fuel oxidation we see a net ____ number which means ...

negative

conditions are favorable for reaction to go forward

8

Anaerobically , there is no TCA cycle to burn up the .....

reduced NADH

9

Glycolysis will stop unless ______ is recycled

NADH to NAD+

10

All the fuel we use eventually breaks down to ...

Acetyl CoA

11

What is the source of carbons in the CO2 molecules produced?

Acetyl group

12

The krebs cycle produces ...

2 CO2
3 NADH
FADH2
GTP
and regenerates Oxaloacetate

13

Anapleurotic reactions

intermediates can be synthesized by other enzymes and fed into the TCA cycle to refill it

14

Arsenic poisoning

inhibits dehydrogenases - pyruvate DH, succinate DH as well as other glycolytic pathways
Inhibits both aerobic and anaerobic

15

What metabolites can Acetyl CoA form? outside of the TCA cycle

Fatty acids

16

What metabolites can alpha-ketoglutarate form outside of the TCA cycle?

Glutamate

17

What metabolites can Succinyl CoA form outside of the TCA cycle?

Delta-aminolevulinate

18

What metabolites can Fumarate/oxaloacetate form outside of the TCA cycle?

Aspartate

19

Where does the TCA cycle occur?

in the mitochondrial matrix except SuccinateDH which is in the inner mitochondrial membrane

20

Where is the ETC found ?

the inner mitochondrial membrane and functions by generating a Protein gradient ACROSS inner membrane as electrons pass through it

21

Where is cytochrome C found?

it is water soluble and found in the intermembrane space

22

Where does oxidative phosphorylation occur?

occurs in matrix as protons flow back into matrix along gradient

23

The more negative, the reduction potential, the more likely it will ...

give up electrons

24

Actual reduction potential is a number measured relative to

the normal Hydrogen electrode (NHE)

25

the more positive the reduction potential, the more likely it will ....

Accept electrons

26

Midpoint potential is the voltage at which the redox active group is

50% oxidized and 50% reduced

27

Kd

concentration at which ligand is 50% bound and 50% dissociated

28

pKa = pH when ...

ionizable group is 50% ionized and 50% unionized

29

Km

concentration of substrate at 1/2Vmax