Flashcards in Energy metabolism Deck (43)
What would upregulate the TCA cycle?
What is the toxic effect of CN-?
binds tightly to heme iron in cytochrome oxidase which oxidative phosphorylation
What is your anaerobic fate of pyruvate?
anaerobically, there is no TCA cycle to burn up the reduced NADH
must recycle NADH --> NAD+ otherwise glycolysis will stop
Electrons are a waste product, and they must be disposed of on something other than oxygen
What is the aerobic fate of pyruvate?
electrons are source of Energy
What is delta G?
change in free energy
delta G nott
at standard concentration conditions
delta G nott '
change in Gibbs free energy @ 25 C and pH 7
with regards to free energy, in fuel oxidation we see a net ____ number which means ...
conditions are favorable for reaction to go forward
Anaerobically , there is no TCA cycle to burn up the .....
Glycolysis will stop unless ______ is recycled
NADH to NAD+
All the fuel we use eventually breaks down to ...
What is the source of carbons in the CO2 molecules produced?
The krebs cycle produces ...
and regenerates Oxaloacetate
intermediates can be synthesized by other enzymes and fed into the TCA cycle to refill it
inhibits dehydrogenases - pyruvate DH, succinate DH as well as other glycolytic pathways
Inhibits both aerobic and anaerobic
What metabolites can Acetyl CoA form? outside of the TCA cycle
What metabolites can alpha-ketoglutarate form outside of the TCA cycle?
What metabolites can Succinyl CoA form outside of the TCA cycle?
What metabolites can Fumarate/oxaloacetate form outside of the TCA cycle?
Where does the TCA cycle occur?
in the mitochondrial matrix except SuccinateDH which is in the inner mitochondrial membrane
Where is the ETC found ?
the inner mitochondrial membrane and functions by generating a Protein gradient ACROSS inner membrane as electrons pass through it
Where is cytochrome C found?
it is water soluble and found in the intermembrane space
Where does oxidative phosphorylation occur?
occurs in matrix as protons flow back into matrix along gradient
The more negative, the reduction potential, the more likely it will ...
give up electrons
Actual reduction potential is a number measured relative to
the normal Hydrogen electrode (NHE)
the more positive the reduction potential, the more likely it will ....
Midpoint potential is the voltage at which the redox active group is
50% oxidized and 50% reduced
concentration at which ligand is 50% bound and 50% dissociated
pKa = pH when ...
ionizable group is 50% ionized and 50% unionized