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Flashcards in Glycolysis Deck (31)
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what is GLUT5?

a fructose transporter in the liver or small intestines

1

What is GLUT1?

a housekeeping glucose transporter with a low Km
found in all cells including RBCs which depend solely on glucose for fuel

2

What is GLUT2?

high Km for glucose
presence in liver means liver will take up glucose when blood glucose concentration is raised such as after a meal
allows for the export of glucose from the liver in the fasted state

3

What is GLUT3?

low Km glucose transporter in the brain

4

What is GLUT4?

In adipose and muscle tissues
Insulin dependent translocation to plasma membrane
increases uptake 20 fold

5

What would you expect in a person without GLUT2?

Inability of liver to uptake excess glucose when blood glucose is high
Functioning GLUT2 allows for the export of glucose from liver in the fasted state

6

What links Glycoylosis to other pathways?

Glucose 6 P

7

What is the enzyme in the first committed step of glycolysis?

Phosphofructokinase I
by adding the PO4, the glucose is now trapped inside the cell

8

What is substrate level phosphorylation?

...

9

What does Glycolysis consume?

glucose
2 ADP
2 NAD+
2 Pi

10

What are the products of Glycolysis?

2 pyruvate
2 ATP
2 NADH
2 H+
2 H20

11

Hexokinase IV

only in liver
works when glucose concentration is high
has very high Km = 10^-2

12

PFK1

metabolically irreversible
critical regulatory point
first committed step of glycolysis

13

PFK2

regulatory molecule for PFK1

14

ATP is a substrate AND ???? for ????

Allosteric inhibitor for PFK1

15

NAD+ is reduced to

NADH

16

What is a site for Arsenic poisoning?

AsO4 can substitute for PO4 creating a futile cycle

17

Under aerobic conditions, what is the fate of pyruvate?

Acetyl CoA

18

Under anaerobic conditions in microorganisms, allows Pyruvate conversion to ...

Ethanol

19

Under anaerobic conditions what do our muscles convert pyruvate to?

Lactate

20

what must you recycle to keep glycolysis running?

NADH must be converted back to NAD+ to keep glycolysis running

21

when ATP is needed

glycolysis is activated

22

what inhibits Hexokinase?

excess G6P

23

what ramps up PFK1?

AMP
F26BP

24

what inhibits PFK1?

ATP
citrate

25

What induces Pyruvate kinase ? inhibits?

F1,6 BP - induces

ATP - inhibits

26

What induces Pyruvate dehydrogenase? inhibits?

ADP and Ca+2 - induce
NADH and Acetyl CoA - inhibit

27

what is F 2,6 BP?

allosteric activator of PFK1 that opposes ATP inhibition

synthesized by PFK-2 from F6P
has kinase and phosphatase domain
can be regulated by serine-threonine protein kinases

28

For PFK-2,in skeletal muscle, when a high concentration of F6P is present, this activate the kinase and inhibit the phosphatase thereby

increasing concentration of F2,6 BP and activating glycolysis

29

increased NADH concentration prevents pyruvate oxidation in the TCA cycle and directs ...

pyruvate to lactate