TCA cycle

Question 0

Vitamin precursor of CoA

Answer 0

Pantothenate

Niacin is for NAD
riboflavin is for FAD and FMN

Question 1

what can generate Acetyl CoA?

Answer 1

pyruvate 
acetate 
ketone bodies 
fatty acids 
amino acids

Question 2

Lack of NADH activates flux through which enzymes?

Answer 2

Isocitrate dehydrogenase
a-ketoglutarate dehydrogenase
malate dehydrogenase

Question 3

which enzymes in TCA have Fe-S centers?

Answer 3

Succinate dehydrogenase

aconitase

Question 4

What enzymes in TCA contain bound FAD

Answer 4

succinate dehydrogenase

a-ketoglutarate dehydrogenase

Question 5

water soluble protein found in intermembrane space

Answer 5

cytochrome c

Question 6

what generates the proton motive force?

Answer 6

there is an electrical component and an pH driven component

Question 7

Acetyl CoA and OAA and water =

Answer 7

citrate

via citrate synthase

Question 8

citrate =

Answer 8

isocitrate via Aconitase

reversible

Question 9

isocitrate + NAD+ =

Answer 9

a-ketoglutarate + NADH + H+ + CO2

via isocitrate dehydrogenase

Question 10

a-ketoglutarate + CoASH +NAD+ =

Answer 10

Succinyl CoA + NADH + H+ CO2

via a-ketoglutarate dehydrogenase complex

Question 11

Succinyl CoA + GDP + Pi =

Answer 11

Succinate + GTP + CoASH

via succinyl CoA synthetase

Question 12

Succinate + [FAD] =

Answer 12

fumarate + [FADH2]

via succinate dehydrogenase

Question 13

Fumarate + h2O =

Answer 13

L-malate

via fumarase

Question 14

L-malate + NAD+ =

Answer 14

OAA + NADH + H+

via malate dehydrogenase

Question 15

net reaction for TCA

Acetyl CoA + 3 NAD+ + [FAD] + GDP + Pi + H2O =

Answer 15

CoASH + 3 NADH + [FADH2] + GTP + 2 CO2 + 3 H+

Question 16

Simple combustion of acetate

Answer 16

acetate + 2 O2 + H+ = 2 CO2 + 2 H2o

Question 17

Anapleurotic reaction of Acetyl CoA

Answer 17

fatty acids

Question 18

Anapleurotic rxn of a-ketoglutarate

Answer 18

Glutamate which could be used to make purines

Question 19

anapleurotic rxn of Succinyl CoA

Answer 19

can be used to make d-aminolevulinate which can be used to porphyrins

Question 20

Fumarate and OAA in an anapleurotic rxn can produce

Answer 20

aspartate which can be used to make pyrimidines

Question 21

Arsenic poisoning

Answer 21

inhibits dehydrogenases (pyruvate DH and succinate DH) and Complex IV
affects DH in glycolysis and gluconeogenic pathways
so inhibits both aerobic and anaerobic pathways

Question 22

how is a-ketoglutarate dehydrogenase complex regulated?

Answer 22

AMP activates
NADH inhibits
Succinyl CoA inhibits

Question 23

How is isocitrate dehydrogenase regulated?

Answer 23

Inhibited by ATP and NADH

upregulated by ADP

Question 24

how is citrate synthase regulated?

Answer 24

Inhibited by ATP, NADH and Succinyl CoA

Question 25

how is pyruvate dehydrogenase regulated?

Answer 25

Inhibited by ATP, Acetyl CoA, NADH | upregulated by NAD+ and CoA

Question 26

how is pyruvate carboxylase regulated?

Answer 26

upregulated by Acetyl CoA