Enzyme Activity Flashcards
(13 cards)
What is the chemical equation for the reaction catalyzed by catalase?
2H₂O₂ (l) → 2H₂O (l) + O₂ (g)
What is the enzyme used in this experiment?
Catalase (from fresh potato strips).
What is the substrate in this reaction?
Hydrogen peroxide (H₂O₂).
What is the test for oxygen gas in this experiment?
The glowing splint test – the splint relights.
What is the role of manganese IV oxide in this experiment?
It acts as a non-biological catalyst in the positive control.
What is the conclusion from using manganese IV oxide?
The reaction happens due to a catalyst.
What is the 1st negative control?
Using boiled potato strips instead of fresh ones.
What is the conclusion of the 1st negative control?
Enzymes denature at high temperatures.
What is the 2nd negative control?
Using water instead of hydrogen peroxide.
What is the conclusion of the 2nd negative control?
Hydrogen peroxide is the substrate.
What is the 3rd negative control?
Performing the reaction in a cold environment.
What is the conclusion of the 3rd negative control?
Enzymes work more slowly in the cold.
What observation shows that oxygen is released during the reaction?
Effervescence (bubbles of gas form).
