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Flashcards in FLE Missed Deck (128):
1

Which statement is NOT true of PRIONS?

  1. They can be inherited
  2. They convert normal proteins into pathogenic proteins
  3. They can cause pathology in nearly every organ of the body
  4. They can be acquired through consumption of contaminated food

4

The correct answer is 4 because pathology is only observed in the CNS of the infected person or animal

Although prion infections are usually associated with consumption of contaminated meat, they also can be inherited and encoded in a person’s DNA

Prions convert normal proteins of the brain into pathogenic ones

This conversion is exponential and does take years to decades to complete

 

 

2

Which of the following bacterial populations would have the greatest size after 24 hours?

A) 10 cells with a doubling time of 8 hours

B) 100 cells with a doubling time of 12 hours

C) 1 cell with a doubling time of 2 hour

D) 1000 cells with a doubling time of 24 hours

C

C is the correct answer since in a 24 hour period it will have 12 doubling times if it doubles every 2 hours

Therefore 1 cell will grow exponentially to 4096 cells

  • Answer A will have 4 doubling times in 24 hours.  10 cells will grow to 20, then 40, then 80, and finally 160
  • Answer B will only have 2 doubling times in its 24 hour period.  100 cells will double to 200 and then to 400 cells
  • Lastly, answer D will only double once in a 24 hour period resulting in 2000 cells.

 

3

Which event is an example of bacterial conjugation?

  • A) A virus transferring the DNA for a toxin from Shigella dysenteriae to Escherichia coli
  • B) The lysing of Escherichia coli cells and the DNA being picked up by Shigella dysenteriae
  • C) The passage of RNA from Staphylococcus aureus cells to another strain of  Staphylococcus aureus through a pilus
  • D) The passage of DNA from Escherichia coli to Shigella dysenteriae through a pilus

D

D is the correct answer because the definition of bacterial conjugation is the passage of foreign DNA through a pilus 

​ pilus =a hairlike appendage found on the surface of many bacteria

  • Answer A describes transduction, which is the movement of foreign DNA from one cell to another via a phage
  • Answer B is describing transformation, which is the picking up of naked DNA from the environment
  • Answer C is also describing the passage of nucleic acid through a pilus, but only DNA is passed and not RNA

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4

An increase in CHOLESTEROL has what impact on the plasma membrane?


A) increased membrane fluidity

B) increased solute transport across the membrane
C) decreased membrane fluidity
D) decreased solute transport across the membrane
 

A

This question is asking you to recall knowledge about the fluid mosaic model (see pic)

Cholesterol is an important part of plasma membranes

It adds vital fluidity by disrupting the stacking of the lipid tails forming the membrane

By disrupting the stacking, the lipids can move more freely, and other molecules, such as trans-membrane proteins, can move about

  • C is incorrect because cholesterol is adding fluidity, not decreasing it
  • B and D are incorrect because cholesterol does not impact solute transport across membranes

However, you can also reach the answer rationally by looking at the figure of cholesterol and imagining how it might look within the lipid bilayer

It is very hydrophobic, so it will interact with the lipid tails, but it is also made of multiple planar rings, so it will not stack neatly with the long carbon chains

Since it doesn’t fit neatly into the membrane, it will increase movement.

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5

Based on the data in Figure 2, what is a normal cellular progesterone level?

A) 0.19 ng/ml
B) 0.36 ng/ml

C) 38 ng/ml
D) 0.38 ng

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B

The “normal” cells for this experiment are tested in the first lane, without cAMP or CCCP added

From the information given in Experiment 1, you can see that progesterone is labeled P4, which tells you that the chart above the graph has the data about progesterone concentration

For lane 1, the progesterone level is 0.3 +/- 0.1 ng/ml, giving you a range of 0.2-0.4 ng/ml

 

A is incorrect because 0.19 ng/ml is outside that range

C is incorrect because 38 ng/ml is too large

This might be distracting, however, if you assume that the bar graph is telling you the progesterone concentration, since the bar for lane 1 is at approximately 38

Make sure when looking at a figure that gives multiple types of data that you know which data is coming from where

D is incorrect because 0.38 ng is not a concentration, it is merely a mass

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6

H2O2 vs O2

Bond STRENGTH roughly correlates with bond....?

ORDER! 

O2 is (+2)

H2O2 is (+1)

Therefore O-O bond in O2 is stronger

7

"Chelation"=?

 

"Chelation" describes a particular way that

ions and molecules

bind METAL IONS

Specifically:  involves the formation or presence of two or more separate coordinate bonds between a multiple-bonded ligand and a single central atom

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8

WHAT is the primary type of attraction that:

facilitates complimentary base pair matching?

What other kind of attraction can occur if this primary type is present?

H-bonding

is the primary type of attraction that facilitates complimentary base pair matching

Van der Waals attraction will also occur if molecules are hydrogen bound.

9

What is the synthetic purpose of the thermal decomposition of azobisisobutyronitrile (AIBN) in Step 2a?

 

  • A) two tertiary free radicals for the functionalization of CRGO in Step 2b
  • B) radical cations for the nucleophilic attack of CRGO in Step 2b.
  • C) a stable diatomic nitrogen molecule.
  • D)heat, to increase reaction rate.

 

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A

A correct; tertiary radicals are most easily formed

  • B is incorrect; the radicals formed are neutral
  • C the byproducts are irrelevant to the further reactions
  • D Branched vs. unbranched carbon chains presents no strong advantage to subsequent steps nor to the function of the biosensor.

10

In which solvent is P4 LEAST soluble?

 

A) H2O

B) CH3CH2OH

C) CH3COCH3

D) C6H6
 

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A

  • P4 is nonpolar; all bond dipole moments “cancel” each other out
  • Water should be immediately recognized as the most polar of all solvents in choices A-D, and hydrocarbons as the least polar
  • Alcohols are of intermediate polarity
  • Answer C is a ketone, which is relatively non-polar, but more polar than benzene, Answer D

Therefore, the most polar, and therefore LEAST likely to dissolve P4 among the choices listed is water, Answer A

11

What are the most likely sources of human dietary PHOSPHORUS?

A)protein and hydroxyappetite

B)protein and amino bisphosphonate

C)DNA and RNA

D)phosphoric acid and calcium

C

Nucleic acids are dietary sources of phosphorus due to the presence of the phosphate backbone 

RNA and DNA are nucleic acids

  • Although “Diets rich in protein and calcium” were mentioned as diets that would likely meet phosphorus requirements, protein and calcium do not themselves contain any phosphorus
  • A is incorrect because proteins do not contain phosphorus
    • there are no biological amino acids that incorporate protein

12

What is the EMPIRICAL formula for HYDROXYAPATITE?

Ca5(PO4)3OH

13

 

A) Pi electrons of the phenyl ring and the tertiary hydrogen

B) Pi electrons of the cyclohexenyl ring and the methyl group

C) Hydroxyl groups, epoxide oxygen lone pairs, and nitrogen lone pair

D) Only the lone pairs of the epoxide oxygen

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C

For complex formation between a molecule and an antigen binding fragment, or for almost any binding interaction described in biochemistry, the interactions are intermolecular in nature

In other words, the process is not breaking and forming new covalent bonds, but causing attractions between molecules to hold them into a binding site

  • Further, hydrogen bonding is the most widely used and strongest of the intermolecular forces
  • There are four locations on morphine that can participate in H-bonds:
    • the two hydroxyl groups (donors or acceptors)
    • the lone pair on the epoxide oxygen (H-bond acceptor only)
    • the lone pair on nitrogen (H-bond acceptor only)

14

In which concentration range would the paper-based lateral-flow test strip be most effective as a quantitative tool for predicting blood morphine levels based on test line intensity?

A) 1.0 – 100 ng/mL

  • because the color intensity is highest over this range

B) 1.0 – 100 ng/mL

  • because the difference between test and control line intensities is positive and has the greatest magnitude

C) 0.1 – 1.0 ng/mL

  • because over this range the control test line color intensity is the most consistent

D) 0.1 – 1.0 ng/mL

  • because over this range the intensity vs. morphine concentration plot has the greatest slope

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D

  • For a test to be a good candidate for quantitative measurements, (as opposed to qualitative test where the goal is to determine “morphine present” or “morphine absent”)

the observable response must:

VARY SUBSTANTIALLY and in a CONSISTENT DIRECTION

In the 0.1 to 1.0 ng/mL region:

  • the relative color intensity increases from about 1 to 25 in near-linear fashion
  • ΔYaverage per ΔX  can be estimated to see that this range has a large slope compared to the other regions.

15

If a sample is analyzed and the line intensity registers a relative color intensity of 15, what is the approximate molarity of morphine in the sample?

The molecular formula of morphine is C17H19NO3

HINT: MW=285 g

A) 2.4 × 10–12 M
B)2.5 × 10–9 M

C) 2.4 × 10–6 M
D) 1.1 × 10–9 M
 

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B

In Figure 4, a relative color intensity of 15 corresponds to a morphine concentration of 0.7 ng/mL

 

 

  • If one round 285 grams to 3 x 102 grams, the calculation becomes:

(7 x 10-1 ng/mL)

X (1 g/ 109 ng)

X  (1 mol/3 x 102 g)

X (1 x 103 mL/L)

= 2.33 × 10–9 M

 

16

See picture

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∆G’ = ∆G°’ + RTln [Products]/[Reactants]

=0.4 + (8.31)(310)(ln[0.01])

17

A PEPTIDE BOND differs from

most OTHER chemical bonds in that it features....?

What does this feature PREVENT?

RESONANCE between the C-O and C-N bonds

PREVENTING
FREE
ROTATION!!!

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18

When a SPRINTER is running:

What is the SOURCE of the work done to accelerate Sprinter? 

The GROUND is doing work on the SPRINTER 

By definition, the force doing work on an object must be a force ON the object

The GROUND is exerting a force ⇒ runner

Runner's LEGS are exerting a force ⇒ ground

19

A certain medical device converts the KE of high-velocity metal beads into HEAT energy

If 100% conversion efficiency were possible:

What MAXIMUM theoretical heat energy can be produced by a single chromium bead with:

  • a VOLUME of 1.0 x 10-4 cm3 
  • a VELOCITY of 2.0 x 104 m/s?

(Density of Cr = 7,140 kg/m3)

1.4 x 102 J

Begin with the formula KE = 1/2mv2

  • Density = mass/volume
    • so mass = density*volume
  • Substituting mass into the first equation we get:

KE = (0.5)(density)(volume)(velocity)2

However!!

  • Volume is given in cm3 
    • and must be converted to m3 

Using dimensional analysis:

1x10-4 cm3 x (1m/100cm)3 

= 1.0 x 10-10 m3

Now one can simply solve:

=½(7.1 x 103 kg/m3)(1.0 x 10-10 m3)(2.0 x 104m/s)2

This simplifies to:

= (3.5 x 103)(1 x 10-10)(4.0 x 108)

  • = (3.5 x 10-7)(4.0 x 108)
    • = 14 x 101 
      • = 1.4 x 102 Joules

20

For an acid-base equilibrium at 

CONSTANT TEMPERATURE:

which of the values listed is CONSTANT?

A) pH

B) Concentration of the conjugate base

C) Concentration of the weak acid

D) pKa

D

pKa is the ONLY constant among the answers

The acid-base equilibrium constant, Ka, is a constant for a GIVEN REACTION at a GIVEN TEMPERATURE

...Therefore the negative log of that constant would ALSO be a constant!

 

The pH of the solution is dependent on the concentration of acid and base in the equilibrium

  • making Answer A incorrect

The concentration of both the acid and the conjugate base can VARY depending on the reaction

  • making both Answer B and C incorrect

21

Titrating aqueous AMMONIA with HCl 

would produce a titration curve that looks like WHAT?

Hydrochloric acid is a STRONG acid,

while ammonia is a WEAK base

 

Because the titration starts out with

just the weak base present:

we need a plot that starts out basic, but not strongly basic

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22

In the synthesis of tri-substituted oxazolidines the ACETYLENE acts as a:

 

A) Bronsted acid

B) Bronsted base

C) Lewis acid

D) Lewis base

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C

The acetylenes contain an electron-deficient pi-systems making the double bond ELECTROPHILIC and susceptible to nucleophilic attack

23

 "During normal growth, SOS pathway genes are repressed by LexA, which binds to the SOS box in the operator on either the coding strand or the template strand of the gene"

Q: The nucleotide sequence of the SOS box is most likely:

A) GAACGTTC

B) TATAAA

C) TAGTTGAT

D) GCGCCC
 

A

  • In the passage, it was stated that LexA can bind to either the coding strand or the template strand of the SOS box

This tells you that the SOS box must be a PALINDROME!

  • Remember that DNA sequence palindromes read the same 5’-3’ on BOTH sides

In answer A, 5’- GAACGTTC- 3’

the compliment strand is 3’ –CTTGCAAG- 5’

​...which is IDENTICAL when read 5’-3’

24

Suppose a person holds a mass m in their hand

with their elbow bent and their forearm

in a neutral horizontal position

If both muscle groups are flexed simultaneously, the system will be in equilibrium when the triceps muscle group exerts a force equal to:

 

A) exactly 80m

B) one-half the force exerted by the biceps muscle group.

C) twice the force exerted by the biceps muscle group.

D) less than twice the force exerted by the biceps muscle group

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D

When a person holds a mass steady with a bent elbow, the forearm is PREVENTED from moving either up or down

The MASS also acts DOWN on the forearm

at a lever arm of 40cm

The forearm itself has mass!

which can be assumed to act downward at approximately the center of the forearm

Therefore the bicep must counteract:

  1. the torque due to the weight of the mass
  2. torque due to the weight of the forearm itself
  3. whatever force/torque the tricep is exerting
  • Therefore, when in equilibrium the tricep must be exerting LESS than twice the force of the bicep—

due to the co-directional torque resulting from mass m

25

 What aspect of the BF3 molecule

makes it a Lewis acid?

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In the Lewis structure of BF3:

Boron only has access to 6 electrons

...instead of the 8 necessary to make a (desired!) OCTET  

Therefore it is a Lewis Acid

VERY WILLING TO ACCEPT ELECTRONS 

from an electron pair donor (Lewis Base)

26

At physiological conditions, the autoionization constant of water (Kw) is slightly above 1 x 10-14

If TEMPERATURE is increased by a factor of FOUR

 and PRESSURE is INCREASED to 25 MPa:

Kw is INCREASED by a factor of 100

Q: How does the pH of water at this elevated temperature and pressure differ from pH under physiological conditions?


IT DECREASES BY ONE pH UNIT

 

At room temp:             Kw = 1 x 10-14 

This value increases by a factor of 100

which would be 100 x  10–14

or 1 x 10–12

At the conditions stated in the problem:

  • [H+][OH ] = 1 x 10–12 
    • AND [H+] = 10–6  M

From this we see that pH = 6 

(pH has decreased by one pH unit) 

27

Do Newton's Laws also apply to SPRINGS? 

YES!

28

Many dynamometers employ a spring to provide resistance as grip strength is assessed

Q: If the dynamometer measures a force of 500 N after the spring is compressed 4 cm, how much potential spring energy is stored in the system?

A) 10 J

B) 20 J

C) 100 J

D) 200 J
 

A

First deduce the spring constant, k

Hooke’s law: F = kx 

  • k = F/x
    • = 500N/0.04m
      • = 12500 N/m

Then use the formula PEspring = ½ kx2

PEspring = ½ (12500) (0.04)2 = 10 J

29

When a repair sequence is first joined to the undamaged portion of the DNA during homologous recombination:

The JUNCTION points

between damaged and undamaged DNA

form a temporary four-stranded moiety known as a...?

Holliday Junction

a Holliday junction is a branched nucleic acid structure where four double-stranded domains are joined together. Holliday junctions are an intermediate in genetic recombination

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30

Pt2+, NH3 Cl 

Lewis ACID or Lewis BASE?

Pt2+ is a Lewis acid

NH3 and Cl are Lewis bases

31

A study reports that the majority of patient falls in which the patient strikes a hard ground surface with a velocity less than or equal to 5.0 m/s do NOT result in serious fractures or other injuries

Q: Ignoring air resistance, falls from which height are UNLIKELY to cause injury?

A) 0.5 m

B) 1.25 m

C) 2.5 m

D) 5.0 m
 

B

  • One can use v = √2gh  to solve this problem, OR

You can simply intuit that if a Vfinal of only 5m/s is attained, the person could only have been in free fall for:

  • HALF A SECOND! 
    • Because one FULL second of free fall changes velocity from 0 ⇒ 10 m/s

∴ traveling at an AVERAGE velocity of 2.5 m/s for 0.5 seconds gives a fall distance of 1.25 m, or Answer B

32

γ-Hydroxybutanoic acid (GHB) is commonly known as the “date rape” drug

HO-CH2CH2CH2-COOH

GHB metabolism involves TWO oxidations

  1. First by an alcohol dehydrogenase
  2. Then by an aldehyde dehydrogenase

Q: If one equivalent of each enzyme is available, the FINAL structure of the metabolite will be...?

γ-Hydroxybutyric acid has the structure:

HO-CH2CH2CH2-COOH

Alcohol dehydrogenase oxidizes the alcohol to give:

 ⇒ HOOC-CH2CH2-CHO ⇒

Aldehyde dehydrogenase oxidizes the aldehyde to give:

HOOC-CH2CH2-COOH

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33

One of the major accomplishments of the SENSORIMOTOR stage is the acquisition of.....?

Object Permanence 

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34

 During the PREOPERATIONAL stage, children acquire _________and engage in  _____ ____

 

Acquire LANGUAGE

Engage in PRETEND PLAY

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35

 During the CONCRETE OPERATIONAL stage, children acquire both _______ and _________

 

 CONSERVATION and REASONING

 

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36

During DIASTOLE, the heart is not _______ ;

all chambers are ________

During diastole, the heart is NOT CONTRACTING;

all chambers are RELAXED

 

(FILLING WITH BLOOD)

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37

If something is "FEMINIZED,"

that just means that.....?

Use Poverty as an example

If something  is "feminized,"

that just means that

MORE women are doing it

(in this case, more women are living in poverty means poverty is eing "feminized")

38

Deuterium has an extra ______ compared to the 1H proton, and thus a larger at WHAT?

As a result, how does D2O compare to H2O?

Deuterium has an extra neutron compared to the 1H proton,

and thus a larger atomic mass

As a result, deuterium oxide must have a different molecular weight than water

39

Phosphorylation is a common mechanism for doing WHAT to an enzyme?

Phosphorylation is a common mechanism for

activating or inactivating an enzyme

40

What is the most likely explanation for how PHOSPHORYLATION influences 

activation or inactivation

The CHARGE on the phosphate:

 creates a repulsive interaction, resulting in a conformational change in the enzyme

41

A pro-inflammatory cytokine cannot work ANTAGONISTICALLYagainst

another pro-inflammatory cytokine BECAUSE?

Becuase they will both

stimulate inflammation

42

Why can't an anti-inflammatory

cytokine work SYNERGISTICALLY

 with a pro-inflammatory cytokine?

Because one decreases (anti)

and

one increases (pro) inflammation

 

43

Can  Pro-inflammatory cytokines can work together (synergistically)?

 

 

 

YES!

but that would only increase inflammation further

44

ANTI-inflammatory cytokines are often UPregulated in the presence of increased WHAT?

Why is this?

 In the presence of PRO-inflammatory

cytokine production

because antagonists control the amount of inflammation produced

45

During prolonged strenuous exercise, _____ produced by anaerobic glycolysis is transported to the ______, where it is converted to _____ and eventually metabolized

  • This metabolism involves  the conversion of _____ to _____ in the LIVER and metabolism of ______ in the MUSCLE

During prolonged strenuous exercise, lactate produced by anaerobic glycolysis is transported to the liver

where it is converted to glucose and eventually metabolized.  This process involves the conversion of:

lactate to glucose in the liver and metabolism of glucose in the muscle. 

 

46

The anomeric position of a sugar is an "______ _____"

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The anomeric position of a sugar is an invertible epimer

47

The mechanism of β-galactosidase is known to be a double displacement hydrolysis

Given this information, what is the most likely stereochemical outcome for the anomeric position of β-galactosidase?

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retained

A displacement reaction generally inverts stereochemical configuration, such as in an SN2 reaction.

A double displacement hydrolysis would involve two sequential inversions of configuration

which would lead to an overall retention of configuration

48

  • Some accelerometers use springs rather than electrical current to measure acceleration
  • In a device designed to measure left-right acceleration only, a spring, free-floating seismic mass, and transducer are arranged as shown below

If the net acceleration of the seismic mass is to the RIGHT,

how do the forces of the spring and the transducer on the seismic mass compare?

  • The force of the spring on the seismic mass is larger than the force of the transducer on the seismic mass
  • The force of the transducer on the seismic mass is larger than the force of the spring on the seismic mass.
  • The forces of the spring and the transducer on the seismic mass are equal according to Newton’s Third Law
  • The forces of the spring and the transducer on the seismic mass are equal according to Newton’s First Law.

     

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The force of the spring on the seismic mass is LARGER than the force of the transducer on the seismic mass

TDLR: Since accel (caused by spring) of mass is greater than the acceleration caused by transducer, the force of the spring must ALSO be greater ("O.S.D.")

​​For this problem you must consider the forces acting on the seismic mass

There are only two forces:

  1. the force from the spring which is pushing it to the right
  2. the force from the transducer which is pushing it to the left

Because the stem says specifically that the device is accelerating to the right,

the system is NOT in equilibrium

∴ The Fnet must ALSO be to the RIGHT

This means that Fspring > Ftransducer

49

A new bacteria is discovered that utilizes a novel nucleotide, designated as H.

H has replaced guanine in the bacteria’s genome and base pairs with cytosine.

This bacteria is highly successful at evading the host’s immune defenses because:

A) The bacteria’s genome will not be recognized by antibodies because the host is unfamiliar with the H nucleotide

B) Due to the dramatic change in genome structure, the bacteria produces new proteins which are undetectable to the innate immune system

C) Restriction endonucleases that target non-self DNA molecules and degrade them have very specific cleavage sites, and the H substitution disrupts this functionality

D) The new nucleotide alters the surface proteins of the bacteria to mimic host cell proteins, enabling the bacteria to utilize cell surface receptors and enter the cell undetected

C

  • The newly discovered bacteria has a genome that uses ATCH instead of ATCG, but no other changes are listed
  • H basepairs with C, so you can assume that it behaves in most respects just like G does
  • C is correct because one way that organisms fight invaders is through restriction endonucleases
    • that recognize DNA sequences in genomes that are “non-self.”
  • These endonucleases have very specific restriction sites
    • (because it would be very dangerous to have endonucleases without specific sites, since they could cleave any DNA)

If an endonuclease recognizes CATATG, it will NOT recognize CATATH

  • Even though this sequence is still a palindrome (when double stranded), the endonuclease will “read” H differently than G and will not cleave that DNA
  • Then the bacteria can continue replicating within that cell, and the infection can spread

50

A typical blood bicarbonate concentration is 26 mM

What must the carbonic acid concentration be to achieve a pH of 7.0

if the Ka for carbonic acid at physiological temperature is 9.94 ×10–7?

 

Use Henderson-Hasselbalch equation: 

 pH = pKa + log( [HCO3] / [H2CO3] )

 

 First, convert the Ka given to pKa

Just round the Ka up to 10

pKa = -logKa

pKa = -log(10 x 10-7)

  • = -log(1 x 10-6) = 6
    • 7.0 = 6.0 + log(26mM/x)

1.0 = log(26mM/x)

or 26mM/x = 1 x 101

or 26mM/x = 10

x = 26mM/10, therefore x = 2.6

51

What does the term "ANHYDRASE" imply wrt what's happening in the reaction?

“anhydrase” implies

removal of water

52

What effect would consumption of a carbonated beverage (which leads to excess CO2) have on blood pH in the absence of carbonic anhydrase ("anhydrase"=removal of water)?

The pH of the system at equilibrium would be WHAT  compared to if carbonic anhydrase (which is an enzyme/catalyst) were present? 

the pH would be the SAME as it would have been if the enzyme were present

 

Carbonic anhydrase, being an enzyme, is a catalyst, which speed up a reaction,

....but does NOT!!! change the equilibrium concentrations of

reactants and products

53

5’ GGAUCCUGAAC 3’

  • Suppose the RNA strand shown is a segment of viral genomic DNA
  • Using this RNA as a template, the enzyme reverse transcriptase would produce which of the following nucleotide polymers?

A) 3’ CCUAGGACUUG 5’
B) 5’ GGATCCTGAAC 3’
C) 5’ GUUCAGGAUCC 3’ 3’ CAAGUCCUAGG 5’
D) 5’ GTTCAGGATCC 3’

     5’ GGAUCCUGAAC 3’

D) 5’ GTTCAGGATCC 3’

  • To answer this question, you have to remember that reverse transcriptase is an RNA dependent DNA polymerase

as such, it will generate a DNA molecule!

  • This means that the answer must contain “T” instead of “U”

It must also be COMPLIMENTARY to the genomic DNA given in the question,

5’ GGAUCCUGAAC 3’

  • Answer D is the complimentary DNA strand to the RNA strand given

54

Suppose a molecule composed of atoms A and B features a PERMANENT DIPOLE

This dipole is mostly likely the result of WHAT wrt electrons in the molecule?

UNSYMMETRICAL/ UNEQUAL DISTRIBUTIONS 
of electrons within the molecule

55

What is most likely to occur in mesophilic proteins at high temperatures?

The mesophilic proteins will likely become:


A) denatured due to a loss of primary structure

B) denatured due to the melting of hydrogen bonds

C) denatured due to the breaking disulfide bonds

D) altered due to an adaptive change in quaternary structure
 

HINT: Mesophiles= organisms that live in moderate environments

B

Mesophiles are organisms that live in moderate environments

  • As such, they have not adapted to high temperatures
  • B is correct because temperatures above the melting point of a protein will cause denaturation
    • which will melt the hydrogen bonds that hold secondary and tertiary structure together
  • C is incorrect because disulfide bonds are also COVALENT
    • so will not be affected by heat

56

Does VELOCITY change an object's INERTIA?

NO!!!

57

TRANSCRIPTION occurs in the _______ of the _____

TRANSLATION  occurs in the _______

TRANSCRIPTION occurs in the nucleoplasm of the nucleus

TRANSLATION  occurs in the cytoplasm

58

Protein-based vaccines stimulate an immune response because they?

feature antigens that are similar to the

antigens on the NON-self-cell

59

Translation proceeds from the _’ to the _’ end

Translation proceeds from the 5’ to the 3’ end

You BUILD an pyramid from the top down!

60

Describe: 

THE FACIAL FEEDBACK HYPOTHESIS THEORY

Also give an example

is the theory that expression of an emotion ALSO activates the experience of that emotion

 

Ex: Expressing a ‘happy’ body language in one’s face should engage the experience of happiness

61

In the field of PSYCHOLOGY, what does "VALENCE" mean?

Refers to the intrinsic:

  • PLEASANTNESS (POSITIVE valence)

OR

  • UNPLEASANTNESS (NEGATIVE valence)

of an event, object, or situation

62

In the SOCIAL CONFLICT THEORY, what does

the "second shift" attempt to EXPLAIN?

“second shift” is a term to:

explain the unequal division of labor in the HOUSEHOLD  between women and men

63

Describe "PARTICIPANT OBSERVATION" in research methods

 Participant observation requires researcher directly participate in the social phenomena being studied.

64

What is the storage site for EXPLICIT memories?

What about IMPLICIT memories?

explicit memories are thought to be

stored in the hippocampus

 

The cerebellum is thought to be the

storage site for implicit memories

65

A correlation coefficient  (r2 value of .2 indicates WHAT?

 

twenty percent (20%) of the variability in _________ can be attributed to __________

66

Sociological Theories:

Define STRUCTURAL FUNCTIONAL THEORY

Sees SOCIAL STRATIFICATION as

functional for society and its members

...by REWARDING those who:

  • work the hardest, or
  • contribute the most to society

67

Compare PRIMARY and SECONDARY

REINFORCERS

Primary reinforcers

  • Act to reinforce a behavior WITHOUT previous conditioning

Secondary reinforcers

  • Only take on their reinforcing value as a function of their association with a primary reinforcer

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68

Atomic MASS numbers are used to describe _____s, NOT ______s 

Use D2O compared to H2O as an example of this

Atomic Mass numbers are used to describe atoms, NOT molecules!

 Mass number is an atomic characteristic describing the combined mass of the

protons and neutrons in an atom

 

It would be correct to say deuterium has a different mass number than hydrogen, but NOT to say that deuterium oxide has a different “mass number” than water

69

"_________ definitions" are ways to measure and define a CONSTRUCT

construct  is a variable that cannot be ____ or _____

 Operational definitions are ways to measure and define a construct

A construct is variable that cannot be seen or touched

70

Mental rotation and navigation tasks are accomplished by

 WHAT REGION in WHAT TYPE OF MEMORY?

Mental rotation and navigation tasks are accomplished by: 

the visual-spatial sketchpad

in WORKING memory

71

A runner rounds a curve while maintaining a constant speed.  

Is a net force experienced by the runner as she rounds the curve?


A) No, because her speed is constant
B) No, because she is in an accelerated reference frame
C) Yes, because she is slowing down
D) Yes, because she is changing direction

D

  • Considering C, the problem specifically says that the runner maintains constant speed
    • therefore the “slowing down” indicated in choice C doesn’t match the given information and is incorrect
  • D does make sense because it DOES take a net force to cause an object to change direction

A change in direction means a centripetal acceleration which is directed inwards...

  • and in this case would be caused by friction from the track against the runner’s shoes

72

The proton gradient created by the ETC  provides the energy for conversion of

ADP + Pi⇒ATP

This is an example of what type of energy conversion?

_____⇒_____⇒_____

Electrical potential ⇒ Kinetic ⇒ Chemical

  • The electrochemical proton gradient that exists across the mitochondrial membrane is an example of electrical potential energy
  • As the protons move through the ATP synthase complex they transfer some of that energy to movement (kinetic) energy
  • The ATP synthase complex produces an ATP molecule out of lower energy molecules, ADP and Pi, harnessing the energy of the proton gradient
  • The energy ends up stored in the bonds of ATP
    • an example of chemical energy

 

73

In contrast to globular proteins, some cytosolic proteins (BETTER KNOWN AS...?) 

have significant flexibility due to unfolded domains and the lack of a tightly ordered internal core

A protein with which characteristics will exhibit the greatest flexibility in dilute solution?

  • A) High net charge and low hydrophobicity
  • B) High net charge and high hydrophobicity
  • C) Low net charge and low hydrophobicity
  • D) Low net charge and high hydrophobicity

 

A

high net charge density and a low hydrophobicity

  •  The cytosolic proteins referenced in the stem are called Intrinsically Disordered Proteins (IDPs)
  • Intrinsically disordered proteins, which have a high degree of flexibility, are characterized by a high net charge density and a low hydrophobicity

These characteristics prevent formation of a hydrophobic core

stabilizing the protein

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74

If there are x number of H2B (core) histone proteins in a cell,

how many H1 (linker) proteins are expected?

.5x

H1 as a linker histone and H2B as a core histone

  • Each nucleosome consists of two core histones and one linker histone
  • There are twice as many core histones as linker histones
    • or half as many linker histones as core histones

75

Passive immunity results from:

 

  I. receiving a vaccine

 II. contracting a viral or bacterial infection

III. receiving an injection of antibodies to a specific antigen

IV. an infant receiving antibodies from mother’s milk

III and IV

The immunity is given from another source

and not produced by the patient’s immune system

In both III and IV, the patient’s immune system is never activated

  • III
    • the antibodies are directly injected into the patient’s body
  • IV
    • the mother’s body has produced the antibodies

 

76

What's one type of cell that divides continuously without inducing apoptosis?

Embryonic stem cells

divide continuously without additional stimulation

Most other cells need growth factors or mitogens in order to stimulate cell division and eventually the line will cease dividing

77

How is release of Parathyroid Hormone (PTH) regulated by POSITIVE feedback?

IT ISN'T!!!!!!!

It's regulated by SIMPLE FEEDBACK INHIBITION (by Ca2+ lvls in the blood!)

POSITIVE feedback would imply that increased

PTH levels would cause a decrease in release of PTH, but.....

 

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78

In Blood-Typing, what does the + and - mean?

Would a transfusion b/t:

an A+ donor and an AB- recipient

produce reactive antibodies? 

The “+” or “-"denotes the Rh factor,

which is ANOTHER antigen found on blood cells

 The recipient is negative for Rh and therefore does not possess the antigen

and as a consequence will not have developed self-tolerance to the Rh factor

Because the Rh factor will be viewed as a foreign antigen, Patient Y WILL produce antibodies against it

79

Protein folding involves a transition from a more disordered state

to a more ordered state

Why does this entropic penalty not prevent spontaneous protein folding in vivo?

HINT: Has to do with the protein's surroundings

Because the entropy (S) change to the surroundings is POSITIVE

...resulting in a net negative Gibbs free energy change

 

  • The reduction in the solvation layer is associated with a significant positive entropy

which overcomes the entropic penalty associated

with increased order in the folded state

  • This allows the net Gibbs free energy change to be negative
    • and therefore the process is still spontaneous

80

PROBLEM SOLVING:

Describe "INSIGHT"

INSIGHT refers to:

those “aha!” moments

in which the solution to a problem suddenly becomes clear

81

What tool do "BIG DATA" STUDIES take advantage of? 

Big data studies take advantage of:

the millions of data points generated by 

SEARCH ENGINES & SOCIAL MEDIA

82

Describe the Single-payer plan model of health insurance

What country uses this kind of model?

Single-payer insurance plans typically have one entity such as the government or an insurance provider where citizens pay for their monthly premiums to and the entity pays the medical bills

 

Canada uses this type of model

83

If a face stimulus were presented in the right visual field, which area of the brain would be responsible for processing that information?

Left geniculate nucleus

...of the THALAMUS

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84

What is the 

PRIMARY EXCITATORY NEUROTRANSMITTER

throughout the Nervous System?

Glutamate

85

What is the 

PRIMARY INHIBITORY NEUROTRANSMITTER

throughout the Nervous System?

GABA

86

What is a

 INHIBITORY NEUROTRANSMITTER

found in the Brain Stem & Retina?

GLYCINE

87

Define FUNCTIONAL (OR "GENETIC") REDUNDANCY

Genetic redundancy is a term typically used to describe situations where a given biochemical function is redundantly encoded by two or more genes

In these cases, mutations (or defects) in one of these genes will have a smaller effect than expected from the genes' function

88

In the ENDOCRINE SYSTEM, what 3 glands determine sexual characteristics?

hypothalamus, hypophysis and gonads

89

How do viruses gain entry to a cell? 

Animal viruses gain entrance to cells by binding to receptor proteins on the exterior leaflet of the plasma membrane

90

A given serum sample has a volume of V and a molarity of M.

If the volume of the sample were doubled while the temperature remained constant, what would be the new molarity in terms of M?

.5M

 This uses the formula M1V1=M2V2, a simplification of PV = nRT for two states of the same system

Let’s call the original volume 1 and original molarity 1. 

The new volume is 2 and the M2 or new molarity of unknown

Plugging into the formula we get 1X1=2XM

therefore rearranging 1X1/2=M

so M=0.5

91

How does telomerase lead to cell immortalization?


Telomeres which are too long will never shorten to a critical length,

and DNA replication can therefore occur indefinitely
 

92

What would happen in a cell that lacks telomeres?

without telomeres, the end of the lagging strand of the chromosome shortens with each replication

(leads to premature cell death)

93

Heat is transferred throughout the body as a result of warm blood flowing past the cells lining capillary walls

This is an example of:

  • Conduction
  • Convection
  • Radiation
  • Condensation

CONDUCTION!!!!!!!!

In the case of the cells lining capillaries, the heat of the blood causes individual cellular atoms and molecules to bump into each other

This raises their temperature and the process is repeated as vibrations are passed through the cell to surrounding fluids and tissues

Heat transfer via direct contact of this type is an example of conduction

94

“Load force” is essentially just another term for?

the NORMAL FORCE 

and normal force is pressure times area

95

Chaperone  proteins aid in protein folding by:

providing a pocket to protect unfolded regions of the protein from aggregation

 

Chaperones protect virgin kids from having sex

96

Methanogens are PROKARYOTIC organisms which use carbon sources to generate methane

In what cellular structure are the enzymes necessary for methanogenesis located?

Cell membrane

 

97

Which class of biomolecules is composed of carbon, hydrogen and oxygen in a 1:2:1 ratio?

Carbohydrates

98

The adaptive immune cells most directly responsible for the production and secretion of antibodies are the?

PLASMA CELLS

99

In healthy individuals, which location has the highest blood pressure?

And, is this during systole or diastole?

Lower leg, during systole

100

From a cellular perspective, the most efficient mechanism for gene silencing is at the ______ level

TranSCRIPTIONAL level

Silence it before it's even written

101

p85α and p85β are two regulatory proteins for the PI-3 kinase which share structural similarities, but are encoded for by two different genes. p85α and p85β are:

  • alternative splice variants
  • protein isoforms
  • atomic isotopes
  • recombinant proteins

PROTEIN ISOFORMS

Since p85α and p85β are encoded by two different genes, therefore they cannot be alternative splice variants, which are produced as the result of alternative splicing of the gene product of one gene

Given that these two proteins have similar functions they would fall the category of isoforms

102

Bacterial cellulose is not susceptible to degradation in the body to the same degree as hydroxyapatite

What is the most likely explanation for the integrity of bacterial cellulose?

Enzymes that hydrolyze

β-1-4 glycosidic linkages

are not present in human cells

103

Mechanisms in younger children compensate for the lower diffusion capacity shown in Figure 1

Which of the following is a physiological trait that declines with age and is likely to compensate for decreased diffusion capacity in children?

  • Density of HgB
  • Density of capillaries/kg tissue
  • Resting HR
  • RBC count

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RESTING HEART RATE

The resting heart rate of newborns is 100-160 beats per minute and the average heart rate declines through adolescence

The increase in heart rate would cause blood to flow in and out of the lungs at a faster pace

...thus increasing the opportunity for diffusion of blood gases

104

Are skeletal muscle sarcomeres and cardiac muscle sarcomeres similar in structure?

YES11

105

Based on Figure 1, in the myocytes of wild-type mice treated with H2O2, administration of the drug carbachol will have what impact on cellular NO levels?

  • NO levels will increase
  • NO levels will decrease
  • NO levels will remain constant
  • NO will no longer be produced by eNOS

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NO levels will INCREASE

Using Figure 1, it can be determined that in wild type mice carbachol significantly upregulates cGMP levels over basal levels

In the passage we learn that cGMP is an agonist for the activation of MEK 1/2 so therefore carbachol will increase the activation of MEK1/2

The passage also states that following H2O2 treatment the addition of an MEK1/2 agonist results in a significant increase in NO

Therefore the addition of carbachol will cause an increase in NO production.

106

A man with type A blood and a woman with type B blood have one child who has type B blood, and one child who has type A blood

If the couple has a third child, what is the probability he or she will have type O blood?

25%

The man is type B and the woman is type A

Since they have a child with type B blood, this means the child received the B allele from the man and must have received the O allele from the woman

Recall that O is recessive

So, the woman’s genotype must be AO

The same reasoning from the type A child tells us that the man’s genotype must be BO

When an AO x BO mating occurs, the Punnett square below is used to predict the proportion of each type of progeny

Hence, the probability of obtaining an O child is 25 % 

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107

Suppose an individual from a previously unidentified animal species is discovered and found to have 23 total chromosomes per autosomal cell

This individual is likely to be sterile because:

  • A) mitosis would be irregular given that the chromosomes cannot be duplicated.
  • B) mitosis would be irregular given that each chromosome cannot pair with a homolog.
  • C) meiosis would be irregular given that the chromosomes cannot be duplicated.
  • D) meiosis would be irregular given that each chromosome cannot pair with a homolog.

D

In meiosis, replicated chromosomes align in homologous pairs and one replicated chromosome moves to each pole in meiosis I,

reducing the ploidy from 2n to 1n

This would not be possible with an odd number of chromosomes

108

What does a sarcomere that would produce the greatest tension upon contraction look like?

Muscles generate the greatest tension when they are at “optimal” length

The sarcomere shown illustrates the optimal length:

ALL of the myosin heads can form cross bridges, and

the thin filaments can slide the MAXIMAL amount

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109

Which graph depicts the relationship between sarcomere length and distance from the center of a skeletal muscle? 

(Length=y axis, distance=x axis)

The model predicts that

sarcomeres will be:

  • SHORTER toward the TENDONS
    • ...which are located at both ends of the muscle
  • and LARGER farther away from the tendons

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110

Persons born with limbs of unequal length often undergo surgery to repair this deformity

Suppose fascicle length increased after reconstructive limb lengthening surgery by 5 cm, but the average sarcomere length remained at 3.52 µm

The increase in fascicle length could have been the result of:

  • A) muscle fiber lengthening in regions without sarcomeres
  • B) the addition of approximately 1,400 serial sarcomeres
  • C) the addition of approximately 50,000 serial sarcomeres
  • D) the addition of approximately 14,000 serial sarcomeres

The term serial sarcomere implies that sarcomeres are added in series, in other words:

the linear number rather than the total number is considered

5 cm of muscle length (or fascicle length) was added

There are 3.52 µm/sarcomere

To compare the two, the addition muscle length must be converted to micrometers:  

(See figure)

111

Which relationship best describes the energy conversion in a contracting skeletal muscle?

  • A) Chemical to mechanical
  • B) Chemical to work
  • C) Chemical to mechanical plus heat
  • D) Mechanical to thermal

Chemical ⇒ mechanical + heat 

112

Why is the amine group on S142 unable to form a hydrogen bond with the amine group on the 5-fluoruracil?

 

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Both functional groups have a partial positive charge

113

What impact would a Q217 to N217 mutation have on the activity of UPP?

  • Binding to 5-fluorouracil would strengthen, increasing UPP activity
  • Binding to 5-fluorouracil would weaken, increasing UPP activity
  • Binding to 5-fluorouracil would strengthen, decreasing UPP activity
  • Binding to 5-fluorouracil would weaken, decreasing UPP activity
     

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Binding to 5-fluorouracil would WEAKEN,

INCREASING UPP activity

the binding to 5-fluorouracil will be weaker since the hydrogen bonding distance will be longer after the mutation

114

If 5.0 g of K2SO4 is mixed with 100 mL of water, what will be the MOST ABUNDANT aqueous solute?

  • OH-
  • K+
  • HSO4
  • SO42-

K+

The compound contributes 2 K+ for every SO42– 

initially [K+] = [SO42– ],

so the concentration of K+ will be higher than sulfate and any other species that can form as a result of sulfate accepting H+ from water

115

For the hypothetical reaction shown below, with stoichiometric coefficients X, Y and Z, the rate data shown in the table suggest that the reaction order with respect to nitrogen gas is?

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FIRST ORDER

The first thing to do for this question is to attempt to balance the equation.

Then one can look at the rate data in the table.

As the table shows, doubling the concentration of the nitrogen gas doubles the rate of the reaction

This is only consistent with a reaction that is

first order in nitrogen

 

116

In-vivo lipase activity is most closely associated with which class of molecules?

 

A)β-lactams

B)Triglycerides

C) Amino Acids

D)Triphosphates

 

TRIGLYCERIDES

 

Lipases are enzymes that catalyze the hydrolysis of fatty esters, or lipids

The other options can be disregarded as these structures do not have an ester functional group.  Phosphates can have phosphodiester bonds, but this is slightly different from carbon esters.

117

Describe DISSOCIATIVE AMNESIA

How does it differ from OTHER types of amnesia?

Dissociative amnesia: This disorder is characterized by a blocking out of critical personal information, usually of a traumatic or stressful nature.

Dissociative amnesia, unlike other types of amnesia, does not result from other medical trauma (e.g. a blow to the head)

118

Describe a DISSOCIATIVE FUGUE

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Dissociative fugue is a rare disorder

An individual with dissociative fugue suddenly and unexpectedly takes physical leave of his or her surroundings

and sets off on a journey of some kind

An individual in a fugue state is unaware of or confused about his identity, 

and in some cases will assume a new identity (although this is the exception)

119

A study of female grocery shoppers reported that the majority of respondents felt pressured for time.

Many used heuristics while reading nutritional information about a product, such as briefly perusing front-of- package information rather than reading the nutritional label.

Which concept from the sociology of gender may help explain this finding? WHY?

 

SECOND SHIFT 
because second shift implies a full, eight hour day AFTER regular employment

that includes things duties like shopping

As time is seriously restricted for such women, mental short cuts are likely to be used. 

120

According to Freud, how are the genital and phallic stages most similar?

 

Both include the genitals

as the main erogenous zone

Genital: masturbation

Phallic: Sex w/ opposite gender

121

Which psychological diagnosis would be associated with a person reporting that they had no memory of who they were or where they came from?

 

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Dissociative fugue

is associated with a temporary loss of memory for personal identity

122

SYMBOLIC INTERACTIONISTS examine...?

symbolic interactionists examine

how LABELS affect perception

and create prejudice

123

Define BIOLOGICAL PREPAREDNESS 

Biological preparedness is the idea that

people and animals are inherently inclined to form associations between certain stimuli and responses.

124

What is the ARCUATE FASCICULUS?

The arcuate fasciculus connects:

BROCA'S AREA (MOTOR aspects of speech) to WERNICKE'S AREA (language comprehension)

 

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125

Describe EMPTY NEST SYNDROME

What 2 feelings are characteristic of it?

What is this syndrome generally associated with?

The empty nest syndrome is a feeling of:

GRIEF & LONELINESS

parents might experience as their children move out of the home

It is generally associated with: 

INCREASED MARITAL SATISFACTION

126

Lysine has a positively charged side chain.

 Congo Red had two negatively charged SO3- groups.

What kind of interaction do they make?  

The interaction is +/- attractions

which are IONIC

127

The frequencies of the harmonics are equal to....?

_________ x __________

 the frequencies of the fundamental, times the harmonic number (1,2,3...)

Remember that the third overtone is the fourth harmonic

Thus the frequency will be 4× the fundamental frequency, which is choice D

128

If something is the THIRD "OVERTONE," that means it is...?

the FOURTH HARMONIC!