INTRODUCTION, LAB SAFETY AND EQUIPMENT - RODRIGUEZ BASED

Question 1

measurement of analytes may either use the

Answer 1

conventional unit or the systeme international unit

Question 2

systeme international (SI) unit consist how how many independent base units

Answer 2

7 and each unit represented by a symbol

Question 3

what are the 7 independent base units under SI units

Answer 3

meter (m)
kilogram (kg)
second -s
mole -mol
ampere - A
Kelvin -K
candela - cd

Question 4

a newborn infant or neonate is a child under how many days of age

Answer 4

under 28 days of age (WHO)

Question 5

infants are described of how many yrs of age

Answer 5

0-1 yrs

Question 6

pediatric is described of how many yrs of age

Answer 6

0-18 yrs (birth to 18 y/o)

Question 7

blue quadrant in NFPA

Answer 7

health haazard

Question 8

health haazard criteria

Answer 8

0- normal material
1- slightly hazardous
2- hazardous
3- extreme dange
4- deadly

Question 9

fire hazard criteria

Answer 9

0 - will not burn
1 - above 200C
2 - below 200C
3 - below 100C
4 - below 73C

Question 10

reactivity hazard criteria

Answer 10

0 - stable
1 - unstable if heated
2 - violent chemical change
3 - shock and heat may detonate
4 - may detonate

Question 11

fume hoods must be examined periodically as to ventilation (velocity _____) against inhalation of hazardous and toxic fumes

Answer 11

velocity 100-120 ft/mn

Question 12

it is recommended that safety showers deliver __ gal. mn with what psi

Answer 12

30-50 gal/mn and psi of 20-50 psi

Question 13

what are the poisonous vapors listed in rodriguez

Answer 13

chloroform
methanol
carbon tetrachloride
bromide
ammonia
formaldehyde
merycury

Question 14

what are the human carcinogens listed in the book of rodriguez

Answer 14

BF

benzidine and formaldehyde

Question 15

what are the probable human carcinogens listed in the book of rodriguez

Answer 15

aniline dyes - (auramine-rhodamine)

Question 16

if a chemical spills occurs, the first step must be

Answer 16

assist or evacuate the personnel

Question 17

safety carriers for transport should be provided for bottles of acids and alkalis with volumes greater than ___

Answer 17

500 ml

Question 18

should we store and arranged chemicals alphabetically?

Answer 18

nope - arrange depending on the quantities of chemicals needed and the nature or types of chemicals

Question 19

the maximum working volume of flammable solvents allowed outside the storage cabinets is ____ gallon per room

Answer 19

5 gallon

Question 20

___ must be stored with a provision for second containment because of their tendency to cause damage to the primary compartment

Answer 20

corrosives

Question 21

some chemicals may deteriorate over time and become hazardous with a tendency to explode such as

Answer 21

ether
peroxides

Question 22

acids such as acetic acid and sulfuric acid is not compatible with

Answer 22

oxidizing agents such ash

halogens
nitric acid
potassium nitrate

Question 23

alkali metals such as sodium and potassium is not compatible with

Answer 23

carbon dioxide
chlorinated hydrocarbons
water

Question 24

bromine and chlorin is not compatible with

Answer 24

acetylene
ammoniahal

Question 25

halogens are not compatible with

Answer 25

ammonia acetylene hydrocarbons

Question 26

Laboratory-acquired infections are mostly transmitted tru

Answer 26

parenteral inoculations like needlesticks or contaminated sharps, spills and splashes, ingestion, and inhalation

Question 27

infectious agents that pose the greatest risk are those that are transmitted by __

Answer 27

aerosols

Question 28

lab procedures that create aerosols

Answer 28

pipetting flaming looops agar plates streaking cebtrifugation

Question 29

five most common LAIs

Answer 29

shigellosis salmonellosis tuberculosis brucellosis viral hepatitis

Question 30

are the recommended measures by the WORLD HEALTH ORGANIZATION to avoid covid 19

Answer 30

handwashing physical distance face mask

Question 31

this type of biosafety cabinet only protects the laboratory worker but no provisions for samples

Answer 31

class I

Question 32

this class of BSL cabinet allows room (unsterilized air into the cabinet, to circulate around the workspace, and expose the material within, hense samples are exposed to contamination )

Answer 32

class I

Question 33

this class of BSL cabinet has open fronted cabinet and is only utilized for biological samples

Answer 33

class II type AI

Question 34

percent of air recirculated and exhausted in class II type A ii and iii cabinet

Answer 34

70% recirculated and 30% exhausted

Question 35

most commonly used type of BSL cabinet

Answer 35

class II type AII

Question 36

this BSL cabinet is for both BIOLOGICAL and CLINICAL (infectious) samples and specimens treated with minimal concentration of volatile chemicals

Answer 36

class II type AII

Question 37

percent of air recirculated and exhausted in class II type B1 cabinet

Answer 37

30% recirculated 70% exhausted

Question 38

percent of air recirculated and exhausted in class II type B2 cabinet

Answer 38

0% recirculated 100 exhausted

Question 39

this type of BSL cabinet is for processing chemicals, radioisotopes, and carcinogens aside from biologic samples treated with toxic or hazardous chemicals clue : radioisotope and carcinogets

Answer 39

class II type BII cabinet

Question 40

close fronted BSL cabinet with airtight system

Answer 40

class III cabinetc

Question 41

class III cabinet is also known as

Answer 41

glove-box-cabinet

Question 42

this BSC has gloves attacged and sealed onto the cabinet

Answer 42

class III BSC

Question 43

provides the highest level of safety to the lab worker and can handle BSL 4 agents

Answer 43

class III cabinet

Question 44

this BSC has air coming into and out of the cabinet is sterilized using the HEPA filter

Answer 44

class III

Question 45

arrange the hierarchy of controls of hazard from most effective to least effective

Answer 45

elimination - most substitution engineering controls administrative control PPE

Question 46

is the process where lab practices, control measures, biosafety standards, and the hazardous characteristics of infectious agents are reviewed to prevent lab acquired infections or exposure to high risk pathogen

Answer 46

biorisk assessment

Question 47

bleach should be in contact with the area for decontamination for how many mns

Answer 47

at least 20 mns

Question 48

10% solution of common household bleach inactivates hepatitis B virus for how many mns

Answer 48

10 mns

Question 49

10% solution of common household bleach inactivates HIV virus for how many mns

Answer 49

two minutes

Question 50

10% solution of common household bleach inactivates hepatitis B virus and HIV for how many mns

Answer 50

HBV - 10 MNS HIV 2 MNS

Question 51

the thermometers should be checked periodically against a reference thermometer from the

Answer 51

national institute standard and technology

Question 52

thermometers that differ by more than ____ from the reference thermometers should be disposed of

Answer 52

1*C

Question 53

thermometers should be checked daily for the presence of ___- to ensure accuracy of reading

Answer 53

gas bubbles

Question 54

the speed and revolution per mn of the centrifuge must be checked by

Answer 54

tachometer

Question 55

what are the equipment that must be test with QC annually

Answer 55

analytical balance biohazard hood *** fume hood

Question 56

what are the equipment that must be test with QC daily

Answer 56

biofreezer and refrigerator water bath and heating block

Question 57

what are the equipment that must be test with QC biannual

Answer 57

centrifuge

Question 58

what are the equipment that must be test with QC every 3-6 months

Answer 58

pipet - single and multi channe;

Question 59

__ extinguishers are recommended for computers

Answer 59

halogen hydrocarbon extinguishers

Question 60

the parts of the body most frequently subject to injury in clinical lab are

Answer 60

eyes mouth respiratory GIT

Question 61

heat is effective for decontamination at what temp and time

Answer 61

250*C for 15 mns