Investigating Enzymatic Reactions Practical Flashcards
(9 cards)
How can you investigate now pH affects amylase activity?
1) put a drop of iodine solution into every well of a spotting tile
2) place a Bunsen burner on a heat proof mat and a tripod and gauze over the Bunsen burner until it is 35°C,using a thermometer to measure the temperature.
3) using a syringe add 1 cm³ of amylase solution and 1 cm³ of a buffer solution with a pH of 5 to a boiling tube. using test tube holders, put the tube into the beaker of water and wait for five minutes.
What’s a buffer solution?
a solution which resists changes in pH when acid or alkali is added to it.
What is the equation for rate
Rate=1000/time
What is the equation for rate
Rate=1000/time
How can you investigate now pH affects amylase activity?
1) put a drop of iodine solution into every well of a spotting tile
2) place a Bunsen burner on a heat proof mat and a tripod and gauze over the Bunsen burner until it is 35°C,using a thermometer to measure the temperature.
3) using a syringe add 1 cm³ of amylase solution and 1 cm³ of a buffer solution with a pH of 5 to a boiling tube. using test tube holders, put the tube into the beaker of water and wait for five minutes.
4) next use a different syringe to add 5 cm³ of starch solution to the boiling tube.
5) immediately mix the contents of the boiling tube and add a stop clock
6) use continuous sampling to record how long it takes for the amylase to break down all of the starch to do this use a dropping to take a fresh sample from the boiling tube every 30 seconds and put a drop into a well when the iodine solution remains brownie orange starch is no longer present.
7) repeat the whole experiment with buff solutions of different pH value used to see how pH affects the time taking for the starch to be broken down.
8) remember to control any variables each time (concentration and volume of amylase solution) to make it a fair test.
How can you investigate the effect of pH on amylase?
1) put a drop of iodine solution into every well of a spotting tile
2) place a Bunsen burner on a heat proof mat and a tripod and gauze over the Bunsen burner until it is 35°C,using a thermometer to measure the temperature.
3) using a syringe add 1 cm³ of amylase solution and 1 cm³ of a buffer solution with a pH of 5 to a boiling tube. using test tube holders, put the tube into the beaker of water and wait for five minutes.
4) next use a different syringe to add 5 cm³ of starch solution to the boiling tube.
5) immediately mix the contents of the boiling tube and add a stop clock
6) use continuous sampling to record how long it takes for the amylase to break down all of the starch to do this use a dropping to take a fresh sample from the boiling tube every 30 seconds and put a drop into a well when the iodine solution remains brownie orange starch is no longer present.
7) repeat the whole experiment with buff solutions of different pH value used to see how pH affects the time taking for the starch to be broken down.
8) remember to control any variables each time (concentration and volume of amylase solution) to make it a fair test.
What is the equation for rate?
Rate=1000/time
What is the equation for rate?
Rate=1000/time
What’s a buffer solution?
a solution which resists changes in pH when acid or alkali is added to it.
