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Flashcards in Lab - Biological Evaluation of Foods Deck (34)
1

protein type that contains all the essential AAs in amounts that meet what is required by humans to prevent deficiency

complete protein

2

all animal proteins are complete proteins except __

gelatin

3

protein type that is too low in one or more of the essential AAs to support human growth and maintenance

incomplete protein

4

most plant proteins are incomplete proteins except __

soy

5

Combinations of incomplete proteins that, when
added together, result in a complete protein

complement proteins

6

Legumes and grains: incomplete protein. But they complement each other

legumes: lysine
grains: methionine

7

To optimize meal-stimulated protein synthesis in
muscle, there must be full complement of essential AAs
+ relatively high content of this AA

leucine

8

full complement of essential AAs
+ relatively high content of this AA = maximally stimulate what pathway?

mammalian
target of rapamycin pathway

9

Enumerate two aspects of evaluating protein quality

Amino acid profile (compared to “ideal” pattern)
Digestibility of the protein

10

• normally the reference protein is __because it
contains all the essential AAs

egg

11

Enumerate 3 factors that can limit digestibility of plant protein

cell wall barrier (wall can't be digested)
lipase
trypsin

12

current “gold” standard by FAO/WHO for assessing
protein quality; incorporates digestibility as a factor in quality of proteins

Protein Digestibility Corrected Amino Acid Score

13

Complete proteins can often have PDCAAS values of
__

≥ 1.00

14

Which is false?
A.For PDCAAS, values above 1.0 are truncated.
B.PDCAAS accounts for relative AA differences among
proteins with PDCAAS for 1.0.
C.PDCAAS does not take into consideration protein ileal digestibility.

B

does not account dapat

15

false about Protein Efficiency Ratio
A.Assesses weight gain of growing animals on a
particular protein source (eg rats, chicks)
B.Suitable as an index for human consumption
C.Weight gain is a function of level of protein and
kind of protein

B

not suitable dapat

16

what percent of protein level – maximum growth? for PER

10%

17

3 extraneous factors that may affect PER. Enumerate.

Season
Sensitivity of animals to care
Changes in temperature

18

2 disadvantages of PER. Enumerate.

Weight gain is not constant with protein level
No room for allowance for maintenenance

19

Which is false?
A.Nitrogen balance gives specific information on flux of protein/AA pool within tissues/organs.
B.Carbohydrate and fat are protein-sparing.

A
does not give dapat

20

What is biologic value of protein quality?

Measures how much nitrogen is retained in the body

21

Biologic value of protein quality is not meaningful for individual components of mixed diets. Why?

since limiting AAs can
differ between sources

22

collective term for urinary and fecal nitrogen
losses from the protein diet

metabolic nitrogen

23

collective term for nitrogen losses from the
protein-free diet

endogenous nitrogen

24

negative nitrogen balance indicates ___

protein loss

25

___ content of a meal determines its capacity to
maximally stuimulate muscle protein synthesis

Leucine

26

Which has higher leucine?
A. whey
B. wheat
C. equal

A

27

Which is false?
A.Turnover = lifespan; If unused, becomes excreted
B.Casein may increase muscle protein breakdown
C.Soy has lower leucine content

B

reduce dapat

28

Lactobacillus casei, Lactobacillus rhamnosus - used for microbio assay of what substance?

folic acid

29

Lactobacillus fermenti , Lactobacillus plantarum - used for the ff except:
A.thiamine
B.riboflavin
C.cobalamin
D.pyridoxine

C

niacin dapat

30

[Microbio Assay]
Bacteria and yeast populations are treated with test
sample and quantified by __(method)

turbidimetry

31

[Microbio Assay]
Turbidimetry: __

absorbance @ 540-600 nm

32

scientific name of white rat

Rattus ratus

33

2 most common rat strains used

Sprague Dawley
Wistar

34

false about rats
A.estrus cycle: 4-5 days
B.gestation + weanling rats age: 21-23 days
C.females: 1 month old

C

2-3 mos dapat