Flashcards in Lab - Vit A Det Deck (30)
1 IU Vit A= ___ retinol
Qualitative method of Vit A Det: retinol + esters react with these 2 acids to form a particular color
In quanti det of Vit A, the sample is first __ (process)
Then a (tool) is used
The addition of ____(reagent) produces suspensjon of fluor in solvent
The addition of ___ & __ (rgts) : improve separation
Saturated saline soln
After centrifugation, layers are formed. Organic layer is color __
__ color indicates presence of Vit A
Intensity of color indicates__
Conc of Vit A in sample
If blue/light blue color is observed, how must the findings be reported?
Positive and above 0.5mg/kg
Enum components of HPLC
Reverse phase C18 column
Standard to be used when running with samples with vit A conc below 1.5mg/kg
Low standard 2
Antioxidant added to each standard and sample flask
What does the glass bead do?
Promote even boiling
Role of glacial acetic acid soln
The higher the ___ in the sample, the more precipitate will form
Mobile phase + ratio : identify
Flow rate : identify
Wavelength : identify
Retention time : identify
Injection of standards must be repeated until ___
Peak heights/areas are reproducible
Intersperse with standard solution injections after every __ samples
What is mg(std) in formula for response factor for vit A?
mg (std)=mg of USP std wt in rgts
What is mL(std) in formula for response factor for vit A?
mL of std used in procedure
What is C(std) in formula for response factor for vit A?
Conc of USP Vit A as per certification
What is PH(std) in formula for response factor for vit A?
Peak height/area of standard fr chromatogram
What is 10,000 in formula for response factor for vit A?
Combined dilution factor for vit A std
What is PHsam in formula for vit A content?
Total sample peak height or area of all-trans
What is 100 in formula for vit A content?
Dilution volume of sample
What is W in formula for vit A content?
Weight of sample in g