Lab Exam Flashcards
(168 cards)
Wear proper clothing
wear appropriate shoes no open-toe shoes or sandals no easily flammable garments long hair tied back clothes must reach knees (no shorts/skirts)
Know location, operational details of all laboratory emergency safety equipment, disposal procedures, and evacuation routes.
eye wash in the front and at every sink
shower in the front
dispose of glass in ceramic jar, biohazards in red box, trash in trash
Eating, drinking, or use of tobacco products is strictly prohibited in science labs.
Food & drinks in backpacks
Do not work in the laboratory without the permission of and presence of responsible staff.
Do not work in the laboratory without the permission of and presence of responsible staff.
Perform only assigned laboratory activities, behave in a professional manner
careless/reckless behavior increases safety risks for everyone
Use proper techniques in handling lab materials and equipment
If you are unsure, ask for instructor’s help.
Stay out of the stockroom and preparation areas of the laboratory
Areas are off limits to students
Know what to do in an emergency
a. report all accidents immediately. All personal injuries – regardless of how trivial they may seem at the time, must be reported to the instructor and or professional laboratory personnel. College is required by law to file accident reports.
b. Know the procedure to be followed in case of personal injury:
- report it immediately
- in cases of severe injury, notify campus police (3111) and/or dial 911
c. Know what sorts of accidents are most likely to occur in the lab, as well as what action to take if an accident occurs.
Wash all glassware, dissecting supplies, etc,. that you use when you are finished with your lab exercise.
Wash all glassware, dissecting supplies, etc,. that you use when you are finished with your lab exercise.
Clean your lab table and turn off all hot plates, propane burners, and water faucets. Many students use the lab. No one but you should have to clean up your mess.
Clean your lab table and turn off all hot plates, propane burners, and water faucets. Many students use the lab. No one but you should have to clean up your mess.
Never use flammable liquids such as alcohols, gasoline, or ether near a flame.
Read the label carefully before taking any substance from a bottle. Be sure you are using the correct chemical.
Only take the amount of chemical necessary to conduct the experiment. Using larger quantities won’t make the experiment work any better. Never return unused chemicals to the reagent bottles.
Only take the amount of chemical necessary to conduct the experiment. Using larger quantities won’t make the experiment work any better. Never return unused chemicals to the reagent bottles.
Never throw solid materials (i.e., litmus papers, pH papers, lens paper, toothpicks, animal parts, etc) into the sinks. Dispose of these materials in the appropriate receptacles. Some liquid wastes may be dumped into the lab sinks (check w/ instructor); flush drain with plenty of running water.
Never throw solid materials (i.e., litmus papers, pH papers, lens paper, toothpicks, animal parts, etc) into the sinks. Dispose of these materials in the appropriate receptacles. Some liquid wastes may be dumped into the lab sinks (check w/ instructor); flush drain with plenty of running water.
If you have used a microscope during the lab period, clean all microscope lenses with lens paper when you are finished, clean off the microscope stage, shut off the light. Return to its proper shelf.
If you have used a microscope during the lab period, clean all microscope lenses with lens paper when you are finished, clean off the microscope stage, shut off the light. Return to its proper shelf.
If you have used prepared microscope slides during the lab period, clean off any immersion oil from the slides, return them to their proper box or tray (check the label).
If you have used prepared microscope slides during the lab period, clean off any immersion oil from the slides, return them to their proper box or tray (check the label).
If you are working with live microorgs and spill them on:
a. work surface or floor:
b. skin:
c: clothes
a. work surface or floor: cover w/ paper towel, flood with disinfectant; wait 15 minutes, clean up.
b. Skin: wash area thoroughly with soap and water.
c. Clothes: change clothes; wash and disinfect clothes.
When dealing with bodily fluids (saliva, urine, blood), only work with your own, or wear disposable surgical gloves and avoid skin contact.
When dealing with bodily fluids (saliva, urine, blood), only work with your own, or wear disposable surgical gloves and avoid skin contact.
Disinfect all work surfaces after working with body fluids or microorganisms. Spray & wipe down tables after every lab.
Disinfect all work surfaces after working with body fluids or microorganisms. Spray & wipe down tables after every lab.
Discard all items contaminated with body fluids or microorganisms in designated biohazard containers.
Discard all items contaminated with body fluids or microorganisms in designated biohazard containers.
Wash your hands with soap and water before leaving the lab.
Wash your hands with soap and water before leaving the lab.
FRAMEWORK
arm
base
Stage
stage – supports slide
stage adjustment – clamping devise
stage adjustment knob – for holding and moving the slide around on the stage
Light source
light intensity control – vary intensity of light, under on/off switch
neutral density filter – sometimes added over the light source in the base, or sometime built into the base
Lens system
ocular objectives nosepiece 4x 10x 40x 100x condenser diaphragm
ocular – eyepiece at the top, has 10x magnification
objectives – (lenses), attached to nosepiece
nosepiece – rotatable, moves objectives around
4x – lowest power, actually 40x (10x * 4x)
10x – “low-power”, actually 100x
40x – “high-dry”, actually 400x
100x – “oil immersion”, actually 1000x
condenser – under stage, collects and directs the light from the lamp to the slide being studied, does not affect magnifying power – moves up and down
Diaphragm – regulates amount light that reaches the slide – moves counter/clockwise