Lab Practical: Culture Characteristics Flashcards Preview

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Flashcards in Lab Practical: Culture Characteristics Deck (10):
1

Culture Characteristics

  • unique growth pattern of particular bacteria exhibited on different forms of media such as broth, deep, plates
  • used to help distinguish between types of bacteria and to identify species

 

2

​5 Broth Cultures

  1. Bacillus
  2. Micrococcus
  3. Pseudomonas
  4. Staphylococcus
  5. Unknown

3

Broth characteristics

  • Turbid: cloudy
  • Sediment:  collection of cells at bottom of tube
  • Pellicle: thin film across surface (biofilm)
  • Ring: residue around perimeter of surface 
  • Flocculent: containing small masses or flakes (clumpy)

4

Deep ​characteristics

  • shows the O2 requirements
  • Obligate aerobe: growth only on surface
  • Facultative anaerobe: growth throughout with more at surface 

5

Motiliy Tube characteristics

  • Has less agar used compared to the Deep
  • Semi-solid media
  • motile: growth spreading through semi-solid agar
  • non-motile: growth only along stab line; no spreading

6

Plate characteristics

  • Shows colony morphology
  • Pigmentation: color
  • Appearance:  shiny or dull
  • Texture: smooth or rough
  • size: pinpoint, small, medium, large
  • shape: ​circular, irregular

7

Bacillus (plate)

  • yellow/grayish/brownish
  • dull
  • rough
  • medium
  • irregular

A image thumb
8

Micrococcus (plate)

  • yellow
  • shiny
  • smooth
  • small, pinpoint
  • circular

A image thumb
9

Pseudomonas (plate)

  • green
  • dull
  • rough
  • medium
  • irregular?

A image thumb
10

Staphylococcus (plate)

  • white/grayish
  • shiny
  • smooth
  • pinpoint, small
  • circular

A image thumb