LAB: Microbial Control Flashcards Preview

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Flashcards in LAB: Microbial Control Deck (35):
1

the removal or destruction of ALL LIVING MICROORGANISMS

Sterilization

2

The most common method used for killing microbes is

heating

3

a sterilizing agent is called a __________
liquids and gases can be sterilized by filtration

sterilant

4

Type of sterilization in which food is subjected only to enough heat to destroy the endospores Clostridium botulinum which can produce a deadly toxin.

commercial sterilization

5

control directed at destroying harmful microorganisms. USUALLY refers to the destruction of vegetatice pathogens. Make use of chemicals, ultraviolet radiation, boiling water or steam. Most commonly applied to the use of a chemical to treat an inert surface or substance

Disinfection

6

Destruction of vegetative pathogens on LIVING TISSUE

antisepsis

7

Removal of microbes from a limited area such as the skin around the injection site

degerming

8

kills microorganisms

biocide/germicide

9

inhibit the growth and multiplication of microorganisms

bacteriostasis

10

decay or putrid

sepsis

11

the absence of significant contamination

asepsis

12

factors that influence the effectiveness of antimicrobial treatments

number of microbes
environmental influences
time of exposure
microbial characteristics

13

actions of microbial control agents include

alteration of membrane permeability
damage to proteins and nucleic acids

14

How does altering the membrane permeability aid in microbial control?

Damaging the lipids or proteins of the plasma membrane causes cellular contents to leak into the surrounding medium and interferes with the growth of the cell

15

How does damaging the proteins and nucleic acid of the microbes affect their function?

By damaging these nucleic acids by heat, radiation or chemicals, the cell can no longer replicate nor can it carry out normal metabolic functions.

16

MODE OF ACTION AND RELIABLY ACTIVE AGAINST:
Bleach

oxidizes proteins, membranes, DNA
vegetative bacteria, fungi , viruses
DISINFECTANT

17

MODE OF ACTION AND RELIABLY ACTIVE AGAINST:
60-80% alcohol

denatures proteins
damages lipid membranes
vegetative bacteria, fungi, enveloped viruses
Both antiseptic/disinfectant

18

MODE OF ACTION AND RELIABLY ACTIVE AGAINST:
quaternary ammonium compounds (benzalkonium chloride)

damages lipid membranes
vegetative bacteria (except Pseudomonads), fungi, enveloped viruses
BOTH

19

MODE OF ACTION AND RELIABLY ACTIVE AGAINST:
Hydrogen peroxide

produces reactive oxygen species that oxidize proteins, membranes, DNA
vegetative bacteria (more effective against anaerobes), fungi and viruses
BOTH

20

MODE OF ACTION AND RELIABLY ACTIVE AGAINST:
Phenol (thymol)

denatures proteins, damages lipid membranes
veg. bacteria, fungi and enveloped viruses

21

Active ingredient of : antibacterial hand soap

benzalkonium chloride

22

Active ingredient of : hand sanitizer

ethyl alcohol

23

Active ingredient of : Lysol (white)

bleach

24

Active ingredient of : Lysol

hydrogen peroxide

25

Active ingredient of : Assured

benzalkonium chloride

26

Active ingredient of : Seventh generation

thymol

27

kills microorganisms primarily by coagulating proteins which is caused by breakage of the hydrogen bonds that holds the proteins in their 3d structure. EX. boiling

moist heat sterilization

28

the preferred method of sterilization. HEAT ABOVE THE TEMP OF BOILING WATER. Method is most effective when the organisms either are contacted by the steam directly or are contained in small volume of aq. liquid. WILL KILL ALL ORGANISMS

autoclave

29

What method is used to test antibiotic susceptibility

kirby bauer disk diffusion method

30

Steps on how to perform kirby bauer disk diffusion test

do not touch the plate with the device
4 drops of the four diff types of chemotherepeutic agent concentrated filter paper on the solid agar
MAKE SURE THERE IS EQUAL SEPERATION from one another
Place it right side up

31

forms around the disk after a standard incubation; the larger the zone the more sensitive the microbe is to the antibiotic. Diameter in mm.

Zone of inhibition

32

Bacilllus is most & least sensitive to:

vancomycin
tetracycline
Bacitracin
ampicillin

33

Staphylococcus is most & least sensitive to:

ampicillin
vancomycin
bacitracin
tetracycline (resistant)

34

E.coli is most & least sensitive to:

ampicilin
tetracyline
RESISTANT TO bacitracin and vancomycin

35

Pseudomonas is most and least sensitive to:

RESISTANT TO ALL