Media Inoculation Flashcards Preview

Microbiology > Media Inoculation > Flashcards

Flashcards in Media Inoculation Deck (10):
1

Colony

a visible mass of microbial cells arising from one cell or from a group of the same microbes

2

Pure culture

a single species present in the medium

3

Streak plating

a method of isolating a culture by spreading microorganisms over the surface of a solid culture medium

4

Forms of growth media

  • Broth
  • Slants
  • Deeps
  • Plates

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5

Broth

  • Used to grow large numbers quickly
  • Use sterile loop to transfer sample from broth culture into sterile broth

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6

Slant

  • used for isolating and determining colony morphology but conserves material and space
  • use sterile loop position loop at base of slant surface; zig-zag upward over surface

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7

Deeps

  • may be solid or semi-solid; used to determine O2 requirements and motility
  • Use sterile needle
  • Perform stab in the center of the deep and extend Downward almost to the bottom withdraw needle from same pathway

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8

Plates

  • Used for isolating and determining colony morphology
  • Perform the streak technique
  • Be sure to sterilize the loop between each streak

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9

Distinguish between a pure culture and a mixed/contaminated culture.

one species vs. a variety

10

Recognize the quadrant streak method

Be able to determine whether isolation was achieved