Basic Microscopy Flashcards Preview

Microbiology > Basic Microscopy > Flashcards

Flashcards in Basic Microscopy Deck (25):
1

What microscope is used in the microbiology lab

compound light microscope

2

Magnification of the ocular lens (eyepiece)

10x

3

Magnification of the scanning lens (red)

4x
total 40x

4

Magnification of the low power lens (yellow)

10x
total 100x

5

Magnification of the high power lens (blue/high dry)

40x
total 400x

6

Magnification of the Oil Immersion lens (white)

100x
total 1000x

7

_______ _________: supports objective lenses and rotates them into position

revolving nose piece

8

______: holds slide

stage

9

_______ ______ _______: slide holder and knobs that position slide

mechanical stage control

10

_____ ______ opening in stage that allows light to illuminate

stage aperture

11

__________: controls the amount of light entering stage aperture

diaphragm

12

_________ concentrates light source into a cone of light that passes through diaphragm

condenser

13

______-_________ _____: brings speciment into focus quicly UP AND DOWN

course adjustment knob

14

______ _________ ______: slowly brings speciment into sharper focus

Fine adjustment knob

15

_____: supports coarse and fine adjustment knobs, holds head and stage

Arm

16

______: holds oculars and houses mirrors

head

17

_____ houses light source, supports and stabilizes entire microscope

base

18

_______ ___________: use visible light as a source of illumination

light microscope

19

________ ________: contains more than one magnifying lens. TOTAL MAGNIFICATION = ocular lens x objective lens. MAGNIFIES UP TO 1000X

compound light microscope

20

_________ __________: uses an electron beam as a source of illumination. uses magnets to focus the beam. CANNOT BE USED ON LIVING SPECIMENS.

electron microscope

21

_________ _________ ______: magnifies up to 1 million and useful for studying interior of the cell

transmission Electron microscope

22

__________ ________ ________: gives extremely detailed 3-D view of objects

Scanning Electron Microscope

23

How to Carry a Microscpe

with one hand on the arm and the other hand underneath the base

24

ONLY use _____ __________ to focus when using high power or oil. NEVER USE ______ _________ when viewing a specimen under high power or oil because it may damage the slides and lens

fine adjustment
course adjustment

25

True or False: you can always return to a lower objective after done with the oil immersion objective

FALSE. NEVER.
AFTER MUST CLEAN OBJECTIVE LENS