LabEx2: 9,10,11,12,13 Flashcards
(44 cards)
Lysozyme is an ezyme found in body’s secretion, such as ___1___,____2____,____3_____ and ____4___.
- saliva, 2. nasal secretion, 3. tears and
- hen’s egg(only the concentratedwhite part)
Lysozyme hydrolyzes the glycosidic bond between the ____1____ units of bacterial cell wall. which can cause the bacterial cell to ____2____.
- NAG-NAM (backbone components)
- Osmotic bursting
Lysozyme action is more pronounced and clear in Gram ___1____ than Gram ___2_____
- Gram Positive
- Gram Negative
Gram _______ cells susceptibility to lysozyme action is due to lack of a Protective barrier to the peptidoglycan layer.
Gram positive
In Gram ______ cells have an outer membrane which covers the peptidoglycan layer and shields from enzymatic attack of lysozyme
Gram Negative
__________ and __________, they represent media which routinely used by microbiologist to isolate, identify and confirm presence of specific microorganism from specimen or environment
Starch hydrolysis test:
Selective and differential media
______ have components that inhibit the growth of unwanted bacteria and encourage growth of desired microorganism. Example: Thayer Martin media,Sabouraud’s dextose Agar
Starch hydrolysis test:
Selective media
________ designed for easier detection and isolation of microorganism of interest from other growing on the same plate. Often a particular trait of the microbes is exploited
Starch hydrolysis test:
Differential media
EMB Agar, Mannitol salt Agar and Mc Conkey Agar are both ________ and _______ media
Starch hydrolysis test:
Selective and differential
_______ is a complex carbohydrates (polysaccharide), observed as inclusion bodies in some bacteria.
Starch hydrolysis test:
Starch
______ serves as carbon and energy reserve and can be degraded into individual glucose units and metabolized
Starch hydrolysis test:
Starch
If Bacteria are cultured on media containing starch as sole sugar source, some synthesize and secretes an enzyme called _______
Starch hydrolysis test:
Amylase
_______ belongs to the class of exoenzyme and breaks down starch so individual glucose unit can be transported inside cell easily.
Starch hydrolysis test:
Amylase
The ability to break down starch is examined using ________, it detects presence or absence of intact starch around Bacterial growth area
Starch hydrolysis test:
Iodine reagent
If starch is broken vicinity of bacterial growth, there would be…..
Starch hydrolysis test:
No reaction of iodine with starch
In starch hydrolysis test, Indication of starch hydrolysis by amylase, is seen as a __________ around bacterial growth
Starch hydrolysis test:
Clear area (halo)
In starchhydrolysis test, If intact starch is observed as ___________ around colony which indicate as non-hydrolysis
Starch hydrolysis test:
Blue Color/Dark coloration
_______ is an enzyme produced by many microbes to neutralize the toxic effect of hydrogen peroxide.
Catalase Test:
Catalase
Catalase breaks down hydrogen peroxide to generate ______ and _________.
Catalase Test:
water and oxygen
In catalase test, oxygen release from the peroxide molecule is abserved as ________. immediate ______ is indicative of positive catalase test
Catalase Test:
Bubble
bubbling
Bacterial cells growth is influenced by many ______ and ________ factors
Anaerobic bacterial growth/Gas Chamber:
physical and chemical
______ and chemical factors are required at specifc levels; any deviations from their normal levels and conditions compromises severely bacterial growth
Anaerobic bacterial growth/gas chamber:
Physical and chemical
In anaerobic chamber, available oxygen is depleted due to chemical reactions which are initiated by ________.
Anaerobic bacterial growth/gas chamber:
adding water
The bacteria Clostridium sporogenes is a aerobic or anaerobic?
Anaerobic bacterial growth/gas chamber:
Anaerobic
