Lecture 23 and 24

Question 1

transmembrane differences in proton concentration are the reservoir for the energy extracted from their biological oxidation rxns

Answer 1

chemiosmotic theory

Question 2

how many membranes of the mitochondria

Answer 2

2

Question 3

in mitochrodira, electron transport starts from what

Answer 3

NADH or FADH2

Question 4

in mitochrodira, electron transport goes to what

Answer 4

CoQ, cytochromes, Fe-S cluster proteins

Question 5

segregates the intermediates and enzymes of cytosolic and matrix metabolic pathway s

Answer 5

inner mitochondrial matrix

Question 6

lipid soluble benzoquinone with a long isoprenoid side chain

Answer 6

ubiquinone

Question 7

another name for ubiquinone

Answer 7

coenzyme Q

Question 8

how many electrons can ubiquinone accept

Answer 8

one or two

Question 9

proteins with characteristic strong absorption of visible light due to iron containing heme prothetic groups

Answer 9

cytochromes

Question 10

proteins that contain iron in association with inorganic sulfur atoms and/or with the sulfur atoms of Cys residues in the proteins

Answer 10

iron sulfur proteins

Question 11

complex 1 ETC

Answer 11

NADH dehydrogenase

Question 12

ubiquinone turns to what in complex 1

Answer 12

ubiquinol

Question 13

how many H+ pumped via complex 1

Answer 13

4

Question 14

how many polypeptide chains in complex 1

Answer 14

40+

Question 15

what is complex 2 in ETC

Answer 15

succinate dehydrogenase

Question 16

how many H+ pumped by complex 2

Answer 16

0

Question 17

e transfer in complex 2

Answer 17

FADH2 to Q

Question 18

complex 3 in ETC

Answer 18

cytochrome bc complex

Question 19

e transfer in complex 3

Answer 19

ubiquinol to Cyt C

Question 20

how many H+ pumped via complex 3

Answer 20

4

Question 21

q cycle completes e- transfer from what to what

Answer 21

QH2 to cyt c

Question 22

how many times does q cycle have to run to complete e transfer

Answer 22

2

Question 23

how many e- does cyt c carry

Answer 23

1

Question 24

how many cyt c molecules made from 1 QH2

Answer 24

2

Question 25

what is complex 4 in ETC

Answer 25

cytochrome oxidase

Question 26

e transfer of complex 4

Answer 26

cyt c to o2

Question 27

o2 reduced to what in complex 4

Answer 27

h2o

Question 28

how many H+ pumped for complex 4

Answer 28

4 for each O2 reduced to h2o

Question 29

describes coupling of ATP synthase to an electrochemical proton gradient

Answer 29

chemiosmotic model

Question 30

2 subunits of ATP synthase

Answer 30

F1 and F0

Question 31

1 full rotation of ATP synthase yields what

Answer 31

3 ATp

Question 32

what is required from 1 fill rotation of ATP synthase

Answer 32

9 protons

Question 33

anti porter that moves ADP into the matrix and ATP out of

Answer 33

adenine nucleotide transferase

Question 34

promotes symport of 1 H2PO4- and one H+ into the matrix

Answer 34

phosphate translocase

Question 35

a complex of ATP synthase and both translocases

Answer 35

ATP synthesome

Question 36

catalyzes conversion of superoxide and 2 proton to H2o2 and o2

Answer 36

superoxide dismutase

Question 37

converts h2o2 to h2o

Answer 37

glutathione peroxidase

Question 38

signals reflecting insufficient O2

Answer 38

low ROS levels

Question 39

number of moles of Pi consumed in. phosphorylation to the number of moles of oxygen atoms consumed in oxidation

Answer 39

P/O ratio

Question 40

p/o when NADH is oxidized

Answer 40

2.5

Question 41

p/o when FADH is oxidized

Answer 41

1. 5

Question 42

one NADH pumps how many protons

Answer 42

10 H+

Question 43

1 FADH2 pumps how many protons

Answer 43

6+

Question 44

how many h+ makes 1 ATP

Answer 44

4

Question 45

inner mitochondrial matrix is impermeable to what

Answer 45

NADH and NAD

Question 46

malate aspartate shuttle moves what

Answer 46

NADH equivalents

Question 47

NADH equivalents moved by the malate aspartate shuttle enter where and yield what

Answer 47

complex 1 and p/o of 2.5

Question 48

where do NADH equivalents enter via glycerol 3 phosphate shuttle and yield what

Answer 48

complex 3, yield p/o 1.5

Question 49

what kind of tissue is associated with increase in heat

Answer 49

brown adipose tissue

Question 50

weak acids that carry protons across inner mitochondrial membrane

Answer 50

DNP and FCCP

Question 51

in an uncoupling protein , what inhibits ATP syn

Answer 51

antibiotics

Question 52

e transfer in complex 1 etc

Answer 52

NADH to Q