Lecture--Chapter 13 Flashcards Preview

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Flashcards in Lecture--Chapter 13 Deck (85)
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31

occurs at the replication fork

elongation

32

part of elongation: binds to ssDNA, synthesises short complementary RNA strands

primase

33

part of elongation: complex of helicase, primase, and Pol III.

replisome

34

part of elongation: short, newly synthesised DNA fragments that are formed on the lagging template strand

Okazaki fragments

35

Elongation: DNA Pol III: On leading strand, moves continuously towards the ____ and covalently bonds the next ____.

replication fork; nucleotide

36

Elongation: DNA Pol III: On lagging strand, periodically releases the strand, then reforms ____ at replication fork.

replisome

37

Elongation: DNA Pol III: adds ~___ nucleotides per second.

400

38

Elongation: DNA Pol I: Removes RNA primers by ____ activity.

5'--3' exonuclease

39

Elongation: DNA Pol I: fills in segment with ___

DNA

40

Elongation: DNA Pol I: ____ makes final seal.

DNA ligase

41

Termination: termination sequences on chromosome, opposite oriC.

Ter

42

Termination: proteins bind to ___.

Tus (termination utilisation substance)

43

Termination: Replication forks stop when they encounter ___.

Tus

44

Termination: ____ dissociates, ____ seals the new strands.

replisome; DNA ligase

45

2 intertwined DNA molecules

catenenes

46

DNA gyrase ___ the molecules.

decatenates

47

DNA synthesis has very high ____, 1 error per 108 bases.

fidelity

48

Mismatched ___ is unstable.

base pairing

49

DNA Pol III has 3' to 5' exonuclease activity

proofreading

50

General features of Eukaryotic DNA replication: more complex, though includes many of the same ____.

enzymes

51

General features of Eukaryotic DNA replication: Eukaryotic chromosomes are large, linear, and packed in ____.

nucleosomes

52

General features of Eukaryotic DNA replication: Multiple ____ per chromosome.

origins of replication

53

Numerous polymerases with specialised roles

eukaryotic DNA polymerases

54

catalyse by-pass segments around damaged DNA

lesion-replicating polymerases

55

Elongation: removes the RNA primers

flap endonuclease

56

Elongation: ___ fills in the gap.

DNA pol delta

57

repetitive tandem arrays of 12-16 at the ends of chromosomes

telomeres

58

Importance of telomeres: The ___ end cannot be otherwise replicated.

3'

59

Importance of telomeres: Protect the chromosome from ____, ____, or ____.

degradation; fusions; rearrangements

60

have a 3' overhang at the ends of chromosomes

telomeres