Flashcards in Lecture--Chapter 13 Deck (85)
occurs at the replication fork
part of elongation: binds to ssDNA, synthesises short complementary RNA strands
part of elongation: complex of helicase, primase, and Pol III.
part of elongation: short, newly synthesised DNA fragments that are formed on the lagging template strand
Elongation: DNA Pol III: On leading strand, moves continuously towards the ____ and covalently bonds the next ____.
replication fork; nucleotide
Elongation: DNA Pol III: On lagging strand, periodically releases the strand, then reforms ____ at replication fork.
Elongation: DNA Pol III: adds ~___ nucleotides per second.
Elongation: DNA Pol I: Removes RNA primers by ____ activity.
Elongation: DNA Pol I: fills in segment with ___
Elongation: DNA Pol I: ____ makes final seal.
Termination: termination sequences on chromosome, opposite oriC.
Termination: proteins bind to ___.
Tus (termination utilisation substance)
Termination: Replication forks stop when they encounter ___.
Termination: ____ dissociates, ____ seals the new strands.
replisome; DNA ligase
2 intertwined DNA molecules
DNA gyrase ___ the molecules.
DNA synthesis has very high ____, 1 error per 108 bases.
Mismatched ___ is unstable.
DNA Pol III has 3' to 5' exonuclease activity
General features of Eukaryotic DNA replication: more complex, though includes many of the same ____.
General features of Eukaryotic DNA replication: Eukaryotic chromosomes are large, linear, and packed in ____.
General features of Eukaryotic DNA replication: Multiple ____ per chromosome.
origins of replication
Numerous polymerases with specialised roles
eukaryotic DNA polymerases
catalyse by-pass segments around damaged DNA
Elongation: removes the RNA primers
Elongation: ___ fills in the gap.
DNA pol delta
repetitive tandem arrays of 12-16 at the ends of chromosomes
Importance of telomeres: The ___ end cannot be otherwise replicated.
Importance of telomeres: Protect the chromosome from ____, ____, or ____.
degradation; fusions; rearrangements