Media

Question 1

Blood agar (BAP)

Answer 1

most bacteria

determines hemolytic reactions

Question 2

Chocolate agar (CAP)

Answer 2

Haemophilus and Neisseria

enriched with hemoglobin or IsoVitalleX

Question 3

Phenylethyl alcohol agar (PEA)

Answer 3

selects for gram positive cocci and anaerobic gram negative bacilli

Question 4

Columbia colistin nalidixic acid (CNA)

Answer 4

selects for gram positive cocci

Question 5

Thayer Martin agar

Answer 5

Neisseria gonorrhoeae and Neisseria meningitidis

Question 6

CAMPY Blood agar

Answer 6

Campylobacter species

Question 7

Thioglycolate broth

Answer 7

“back up” for anaerobes

Question 8

Lowenstein Jensen agar

Answer 8

Mycobacterium

Question 9

Middlebrook 7H10 agar

Answer 9

Mycobacterium

Question 10

Petragnani agar

Answer 10

Mycobacterium

Question 11

Bacteroides Bile Esculin agar (BBE)

Answer 11

selects for B fragilis group (black colonies)

Question 12

Kanamycin Vancomycin Laked blood (KVLB)

Answer 12

Bacteroides species (enhances pigment production)

Question 13

Cycloserine Cefoxitin Fructose agar (CCFA)

Answer 13

Clostridium difficile

Question 14

CDC anaerobic blood agar

Answer 14

anaerobes (enriched with hemin, cystine, and vitamin K)

Question 15

Cooked or chopped meat medium

Answer 15

anaerobes

Question 16

Bordet Gengou agar

Answer 16

B pertussis

Question 17

Buffered charcoal yeast extract (BCYE)

Answer 17

Legionella

Question 18

Cystine Glucose agar

Answer 18

F tularensis

Question 19

Fletchers medium

Answer 19

Leptospira

Question 20

Skirrow agar

Answer 20

Helicobacter pylori

Question 21

Thiosulfate Citrate Bile Salts Sucrose (TCBS)

Answer 21

Vibrio species

Question 22

Vaginalis agar (V agar) (human blood)

Answer 22

Gardnerella vaginalis

Question 23

Cystine Tellurite blood (Tinsdale)

Answer 23

Clostridium diphtheriae (black colonies)

Question 24

Loefflers medium

Answer 24

Clostridium diphtheriae (enhances grouping and metachromatic granules)

Question 25

Carbohydrate fermentation with phenol red

Answer 25

``` (+) = yellow (-) = red ```

Question 26

Esculin

Answer 26

``` (+) = black (-) = nondescript ```

Question 27

Hippurate hydrolysis

Answer 27

``` (+) = purple (-) = no change ```

Question 28

Motility

Answer 28

``` (+) = blurred stab line (-) = growth along stab line ```

Question 29

6.5% NaCl broth

Answer 29

``` (+) = turbid (-) = clear ```

Question 30

Phenylalanine or tryptophan deaminase

Answer 30

``` (+) = green surface after 10% FeCl3 (-) = nondescript ```

Question 31

Urea

Answer 31

``` (+) = bright pink (-) = nondescript ```