Membrane Fusion Flashcards Preview

M2M Unit III > Membrane Fusion > Flashcards

Flashcards in Membrane Fusion Deck (30):
1

All cellular membranes are composed from a ____ with ____ facing the outside and interacting with ____.

lipid bilayer; charged residues; water molecules

1

The viral fusion domain is _____ within the viral protein.

folded and hidden

1

Upon receiving _______, the viral fusion domain is exposed and inserted into the target cell membrane.

a specific signal (like a change in pH),

2

Viral fusion proteins contain a _____ at the one end and a _____.

transmembrane domain; highly hydrophobic fusion domain

3

The formation of highly stable SNARE complex drives _______.

membrane fusion

4

____ uses _____ to generate energy needed to disassemble the SNARE complex.

NSF; ATP hydrolysis

5

____ and _____ disassemble the SNARE complex.

NSF; αSNAP

6

VAMP is located in the _________.

transport vesicle

7

Syntaxin and SNAP are located in ________.

the target membrane

8

Name 4 membrane bound organelles.

1) nucleus, 2) endosomes 3) lysosomes 4) mitochondria

9

What proteins help the transport vesicle to fuse with the target membrane?

SNAREs

11

intracellular vesicle transport

transport of proteins in and out of membrane bound organelles without compromising their identity

12

The _____ domain is inserted into the target cell membrane when a virus attacks.

viral fusion

13

Sec1 protein binds and changes ____ to refold the SNARE proteins back into the _________.

syntaxin; active conformation

13

Name 3 enveloped viruses.

1) HIV 2) ebola 3) influenza

14

____ and ____ are located in the target membrane.

Syntaxin; SNAP

15

A transport vesicle needs to be delivered to the ____ via help of _____ that move along ____ or microtubule tracks.

fusion site; molecular motor proteins; actin

16

What does NSF stand for?

N-ethyl-maleimide sensitive fusion protein

18

NSF uses ATP hydrolysis to _____ needed to disassemble the _____.

generate energy; SNARE complex

19

The membrane fusion thermodynamically is highly ______ event, since it needs to overcome the repulsive _____ and dissipate the _____ between two lipid bilayers.

inefficient; ionic forces; hydration

19

________ drives membrane fusion.

The formation of highly stable SNARE complex

20

What does VAMPs stand for?

vesicle associated membrane protein

21

NSF and αSNAP ______.

disassemble the SNARE complex

22

_____ protein binds and changes syntaxin to refold the _____ back into the active conformation.

Sec1; SNARE proteins

24

A ______ needs to be delivered to the fusion site via help of molecular motor proteins that move along actin or _____.

transport vesicle; microtubule tracks

25

What does αSNAP stand for?

soluble NSF attachment protein

27

____ is located in the transport vesicle.

VAMP

28

What does SNAPs stand for?

synaptosome associated protein

29

What does SNAREs stand for?

soluble NSF attachment protein receptor

30

There are three main classes of SNAREs: _____, ______, and ______.

syntaxins, VAMPs, and SNAPs