Misc

Question 1

Why do you heat fix a slide

Answer 1

Dissolves wax

Question 2

What are impression smears from

Answer 2

From external lesion a from tissue removed

Question 3

Disadvantages to impression smears

Answer 3

Few cells collected
Contamination
Only secondary bacterial infection

Question 4

Types of impressions

Answer 4

Single Ar Tzanck method

Question 5

Tznack method

Answer 5

-Make multiple impressions of lesion before cleaning
-Clean with sterile saline
-Multiple impressions after cleaning, debridement and scab removal

Question 6

What to do if there’s a delay in imprinting from collection time

Answer 6

Use scalpel blade used ti expose fresh surface

Question 7

Where are FNAs collected from

Answer 7

Masses-lymph nodes, modular lesions, organs

Question 8

FN biopsy site prep

Answer 8

Surgical prep for microbiological testing

Question 9

FN biopsy from body cavity

Answer 9

Peritoneal, thoracic, joints

Question 10

Equipment for FNA

Answer 10

• 21-25g needle
• 3-20 lm syringe

Question 11

Larger needles are used tissue cores tend to

Answer 11

Aspirate
Has greater blood contamination

Question 12

Things to consider in a tissue biopsy

Answer 12

Location
Accessibility
Nature
+/- clip hair
Site prep

Question 13

Wedge biopsy obtained with

Answer 13

Scalpel

Question 14

Wedge biopsy advantages

Answer 14

Large sized specimen
Portion or entire lesion

Question 15

Punch biopsy sizes

Answer 15

3,4,6,8 mm

Question 16

4 mm punch

Answer 16

Requires no sutures

Question 17

6,8 mm punch

Answer 17

Specimen require sutures

Question 18

Punch biopsy formalin %

Answer 18

10% neutral phosphate buffeted

Question 19

Endoscopic biopsy collection

Answer 19

Specimen collected by endoscopy flushed from the tip of the endoscope

Question 20

Endoscopic biopsy sample prep

Answer 20

Blotted on paper towel on am piece of tobgue depressor to create a splint and placed in fixative when dry

Question 21

Tru-cut biopsy purpose

Answer 21

Biopsy, neoplasia, lesions

Question 22

Approach of try-cut biopsy

Answer 22

Percutaneous
+/- ultrasound
Aseptic needle instered close to site

Question 23

Centesis

Answer 23

Introduction of a needle into body cavity to collect fluid

Question 24

Centesis recording

Answer 24

Total volume collected
Fluid characteristic ( color , turbidity

Question 25

Transtracheal wash

Answer 25

Sample from trachea, bronchi or bronchioles for do of a pulmonary dz

Question 26

Process of tracheal wash

Answer 26

Through endotracheal tube through nasal passages, skin, trachea

Question 27

Why do a transtracheal wash

Answer 27

Minimize pharyngeal contamination of the specimen

Question 28

Percutaneous technique

Answer 28

18-20 g needle through the catheter - clip hair of laryngeal area aspectically - sterilized saline - when animal coughs pull back plunger to collect fluid - lidocaine for local anesthesia - inserted through cricoid membrane asnvabce catheter into tracheal lumen

Question 29

Orotracheal technique

Answer 29

-Fully under, collected through endotracheal tube - No cough, withdraw as fluid is injected

Question 30

Smear from fluid sample prep

Answer 30

Collected in EDTA - abdominocentesis/thoracocentesis

Question 31

Starfish smear

Answer 31

Aspirate on slide and smear sample for ideal viscous