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Miscellaneous Gram Negative Bacteria Flashcards

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1
Q

Disease caused by Pajaroellerobacter abortibovis

A

Foothill abortion in cattle aka epizootic bovine abortion

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2
Q

Where is Pajaroellerbacter abortibovis endemic?

A

California, Nevada and Oregon

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3
Q

How is Pajaroellerobacter abortibovis transmitted?

A

soft tick

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4
Q

How long does immunity against Pajaroellerbacter abortibovis last?

A

1-3 years

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5
Q

Incubation period for Parajorellerobacter abortibovis

A

lasts more than 100 days

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6
Q

When is the fetus most at risk due to Parajorellerobacter abortibovis

A

45 days prior to breeding through 5-6 months of gestation

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7
Q

General pathogenesis of Pajaroellerobacter abortibovis

A

aborted fetus in excellent condition
extensive ascities with excessive fibrin
skin lesions
petechial hemorrhages on mucosal memnranes and thymus

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8
Q

How is Pajaroellerobacter abortibovis diagnosed?

A

tentative diagnosis based on unique fetal pathology
PCR targeting 16S bacterial ribosomal gene

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9
Q

Can Pajaroellerobacter abortibovis be cultured?

A

NO

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10
Q

Staining methods use to visualize Pajaroellerobacter abortibovis

A

gram neg but not well
modified steiner silver stain
immunofluorescence

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11
Q

Is there a vaccine for Pajaroellerobacter abortibovis?

A

yes - live vaccine

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12
Q

Causative agent of red-leg disease in frogs

A

Aeromonas hydrophilia

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12
Q

Causative agent of necrotic stomatitis in snakes and septicemia

A

Aeromonas hydrophilia

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13
Q

Causative agent of skin lesions and hemorrhagic septicemia in eels, cryprids and pike

A

Aeromonas hydrophilia

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14
Q

Causative agent of swin bladder inflammation in pike and grass carp

A

Aeromonas hydrophilia

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15
Q

Causative agent of neonatal septicemia in dogs

A

Aeromonas hydrophilia

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16
Q

Causative agent of mastitis in cattle (misc gram neg)

A

Aeromonas hydrophilia

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17
Q

Causative agent of septicemia in turkeys (misc gram neg)

A

Aeromonas hydrophilia

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18
Q

Causative agent of diarrhea in pigs (misc gram neg)

A

Aeromonas hydrophilia

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19
Q

Causative agent of furunculosis in salmonids

A

Aeromonas salmonicida subsp. salmonicida

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20
Q

Causative agent of ulcer disease in carp

A

Aeromonas salmonicida subsp. salmonicida

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21
Q

What is the habitat of Aeromonas spp?

A

freshwater, sweage, soil

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22
Q

Is aeromonas hydophilia part of the normal flora of freshwater fish?

A

yes
opportunity pathogen

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23
Q

Is aeromonas salmonicida an obligate parasite?

A

yes of salmoid fish

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24
Virulence factors of aeromonas hydrophilia
adhesins exoenzymes hemolysins enterotoxins acetyltransferase system
25
Virulence factors of aeromonas salmonicida
outer protein coat adhesin type I and IV pili type III secretory system
26
Aeromonas spp direct microscopy
gram neg straight rods fluorescent antibody test for salmonicida
27
Are aeromonas spp anerobic or aerobic?
aerobic
28
Culture media used for aeromonas hydrophilia
Blood agar MAC blood agar + 10mg/L ampicillin CIN
29
Culure media used for aeromonas salmonicida
blood agar and furunculosis agar at 25C for 48hrs
30
Aeromonas hydrophilia colony morphology
large, flat, grey colonies betahemolysis punget foul odor MAC - pale colonies
31
How are aeromonas spp, plesiomonas spp, and vibrio spp differentiated?
biochemical testing
32
What miscellaneous gram negative bacteria causes opportunistic infections in fish?
Plesiomonas shigelloides
33
Virulence factors of Plesiomonas shigelloides
heat-stable and heat-liable enterotoxins
34
Culture medium used for Plesiomonas shigelloides
NA BA MAC - nonlactose fermenting
35
Hemolysis of Plesiomonas shigelloides
non-hemolytic
36
Antibiotic resistance of Plesiomonas shigelloides
resistant to beta lactams
37
What disease does vibrio metschnikovii cause in chickens/birds?
enertic disease
38
What disease does vibrio anguillarum cause in salt-water eels and fish?
skin necrosis high mortality
39
Mortlity rate of marine vibrios in fish
up to 100%
40
Culture media used for Vibrio anguillarum
BA + NA base 2.0% NaCl at 20C for 48hrs
41
Culture media used for Vibrio parahaemolyticus
TCBS agar
42
Culture media used for vibrio chloerae and other enteric vibrios
grow on mannitol salt agar 7.5% NaCl
43
Colony morphology of vibrio metschnikovii
smooth, transparent colonies hemolytic grows poorly on MAC
44
Colony morphology of vibrio anguilarum
smooth colonies hemolytic
45
Colony morphology of vibrio parahemolyticus
non-hemolytic green colonies on TCBS ferments mannitol
46
Reservoirs of Streptobacillus moniliformis
rats and small carnivores
47
Disease caused by Streptobacillus moniliformis
Rate-bite Fever aka Haverhill fever
48
Staining methods used for visualizing Streptobacillus moniliformis
does not stain well with Gram Stain dilute carbol fuschin giemsa stain immunofluorescence
49
Culture media used for Steprobacillus moniliformis
brain heart infusion base enriched with 15% blood, 20% horse or calf serum or 5% ascitic fluid
50
Colony morphology of Streptobacillus moniliformis
non-hemolytic round grey smooth glistening
51
Microscopic appearance of Streptobacillus moniliformis
pleomorphic gram neg rod filamentous with knobby irregularities non-motile
52
Biochemical reactivity of Streptobacillus moniliformis
unreactive biochemically
53
Disease caused by ornithobacterium rhinotracheale
respiratory disease in poultry - chickens and turkeys
54
Co-infections commonly found in ornithobacterium rhinotracheale
E. coli Bordetella avium
55
Culturing requirements for ornithobacterium rhinotracheale
5-10% BA supplemented with 5-10% CO2 does not grow on MAC
56
Disease caused by Riemerella anatipestifer
New duck disease aka duck septicemia - ducks and geese
57
Mortality rate of Riemerella anatipestifer
up 70%
58
Characteristics of Riemerella anatipestifer
gram-neg rod: singly, in pairs, occasionally in chains non-motile non-spore forming capsule
59
How to demonstrate Riemerella anatipestifer capsule
india ink stain
60
Transmission of Riemerella anatipestifer
oral or nasal route skin wounds particularly on feet
61
Virulence factors of Riemerella anatipestifer
VampD OmpA CAMP cohemolysin Nicotinamidase PncA LPS biofilm formation
62
Clinical signs of Riemerella anatipestifers
listlessness ocular and nasal discharge sneezing greenish diarrhea ataxia torticollis tremors of head and neck coma
63
Pathology of Riemerella anatipestifer
fibrinous exudate in serosal cavities mucopurulent exudate in nasal sinuses caseous exudate in oviducts localized skin lesions
64
Culture media for Riemerella anatipestifer
BA in 5-10% CO2 No growth on MAC
65
Colony morphology of Riemerella anatipestifer
small, transparent, glistening colonies non-hemolytic
66
How to serologically classify Riemerella anatipestifer
gel precipitation test or agglutination
67
Differential diagnosis of Riemerella anatipestifer
Pasteruella multocida Escherichia coli Enterococcus faecalis Salmonella spp.
68
Treatment for Riemerella anatipestifer infection
sulfonamides in feed or drinking water enrofloxacin ceftiofur
69
Control and prevention of Riemerella anatipestifer
inactivated vaccines live avirulent vaccine management and sanitation

Bacteriology (26 decks)

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