MOLECULAR MEDICINE II

Question 1

refers to chain termination method

based on DNA polymerase reaction

Answer 1

Sanger’s method

Question 2

In Sanger’s method

it requires DNA ________, ________, DNA ________, ______ as substrate and dideoxynucloetide (chain terminator)

Answer 2

DNA template
primer
DNA polymerase
dNTPs as substrate

Question 3

In DNA sequencing
it runs in four separate reactions

each reaction mixture contains substrates (____, ____. ____)

Answer 3

dATP
dGTP
dCTP

Question 4

deoxynucleotide (usual substrate) has a ____________ which is an important attachment site for phosphodiester bond formation

Answer 4

3’ hydroxy

Question 5

occasionally DNA polymerase uses a _______ which terminates further growth in that molecule

random insertion of _________ leaves optimally at least a few chains terminated at every occurrence

Answer 5

dideoxynucleotide
ddNTP

Question 6

method to separate molecules from complex mixture and identify molecule of interest using probe

Answer 6

blotting techniques

Question 7

formally called an DNA blot
analysis of DNA utilizing complementary radioactive DNA probe

Answer 7

southern blot

Question 8

Southern blot

_________ is the magical crystal shoe in Southern blot

Answer 8

dna probe

Question 9

in Southern blot result from point mutation like in ____- wherein there is a mutation from GAG to GTG (from Glu to Val)

Answer 9

sickle cell