Pseudomonas is a gram negative bacteria that is a _____ aerobe
Pseudomonas is extremely ______
What is the main pathogenic species of Pseudomonas?
Pseudomonas is usually a ____ agent with significant____ resistance
usually a secondary agent
Pseudomonas likes to live in ____ poorly aerated environments such as surgery support bags and hoses in anesthetic machines
True/False: Pseudomonas can cause mastitis?
usually via intramammary infusions
Pseudomonas is motile via _____
What virulence factor allows adherance to the respiratory mucosa for Psuedomonas?
outer membrane proteins
and non fimbrial adhesins
True/False: Pseudomonas has a remarkable capacity to adapt to and thrive in diverse ecological niches
Pseudomonas has a virulence factor that leads to ____ formation
True/False: Pseudomonas does not have a capsule
It is encapsulated
What are the exotoxins of Pseudomonas and what are their characteristics?
Exotoxin A- inhibits protein synthesis
Exotoxin S and T- interferes with phagocytosis
Exotoxin U- cytotoxic effect
Exotoxin Y- increases cAMP
Which Pseudomonas exotoxins cause death of the cells that it is associated with?
Exotoxins U and Y
What virulence factor of Pseudomonas is useful diagnostically and helps to protect the pseudomonas against free radicals?
*Pseudomonas is protected due to catalase and superoxide dismuate
What is the Reservoir for Pseudomonas?
Soil, water, feces of normal animals- EVERYTHING
Most infections of Pseudomonas are _____ to compromised hosts
*ex. illness or trauma allows pseudomonas to colonize. It is very opportunistic
What is the pathogenesis of Pseudomonas?
1. reduced normal flora allows bacteria to colonize (usually due to antimicrobial use)
2. adhere to the epithelial cell layer and reproduce
3. Destroys epithelial cells and tissues (pyocyanin)
Where will you see Pseudomonas the most?
What animals are predisposed to Pseudomonas?
breeds with long ears- ex. bassett hounds
breeds with hair in external ear canal
Other than ear infections, what other disease can Pseudomonas cause?
Lower urinary tract infection
*not very common
What can Pseudomonas cause in horses?
*secondary to prolonged treatment with antimicrobials
What does a skin infection of Pseudomonas look like?
Pseudomonas can cause pustular _____ skin lesions
Pseudomonas has caused a particular problem in farmed _____
*mortality 50% due to hemorrhagic pneumonia
What granulomatous inflammation of the skin can Pseudomons cause?
*grape like appearance- little nodules under the skin
What condition can Pseudomonas aeurginosa cause in sheep?
Fleece Rot of sheep
What humans are most suscptible to Pseudomonas infections?
*especially burn victims
What is the best way to prevent Pseudomonas?
Need to decrease the risk of secondary infection
*clean environment, clean and dry ears
Pseudomonas is ____ hemolytic and ___ growing
What is a characteristic of Pseudomonas that helps with diagnosis?
*suppose to smell like grapes
Pseudomonas is oxidase ____
True/False: Pseudomonas is susceptible to antimicrobials
highly resistant to microbials
What give the dark appearance of Pseudomonas on agar?
Pyocyonin turns the plate a dark color
Does Pseudomonas ferment the sugars in a TSI test?
*non fermenters. TSI appears red
Is Pseudomonas lactose negative or positive on MacConkey Agar?
*NO PINK COLOR
What is the best treatment for Pseudomonas?
identify the predisposing factor and eliminate the source of the organism
*Use hygienic practices. Keep endotracheal tubes and anethetic hoses clean