Unit 2- E. coli

Question 1

E coli

Answer 1

Enterobacteriaceae, gram negative rod, facultative anaerobe, flagella, ferment glucose and lactose, catalase positive, gut microflora, antimicrobial resistance

Question 2

E coli Surface Antigens

Answer 2

LPS O antigens, fimbrial F antigens, capsule K antigens, and flagellar H antigens

Question 3

Peritrichous Flagella

Answer 3

Flagella distributed all over the body

Question 4

Large Bowel E Coli

Answer 4

EHEC and EIEC

Question 5

Small Bowel E Coli

Answer 5

ETEC and EPEC

Question 6

ETEC

Answer 6

Enterotoxigenic E coli, F4, F5, F6, and F41 adhesins, attachment to small intestine, heat labile and heat stabile enterotoxin, causes watery diarrhea

Question 7

Heat Labile Enterotoxin

Answer 7

LT, increases cAMP increasing villous secretion

Question 8

Heat Stable Enterotoxin

Answer 8

ST, increases cGMP increasing villous secretion

Question 9

Neonatal Diarrhea

Answer 9

ETEC causes enteric colibacillosis in calf, lamb, and piglet, treat with milk and electrolytes, control with colostrum

Question 10

Post-Weaning Diarrhea

Answer 10

ETEC or EPEC causes watery diarrhea in piglets after weaning

Question 11

EPEC

Answer 11

Enteropathogenic E coli, causes pedestal formation, attaching and effacing lesions, has type III secretion, and adhesin

Question 12

Pedestal Formation

Answer 12

Loss of microvilli and rearrangement of cytoskeleon

Question 13

EHEC

Answer 13

Enterohemorrhagic E coli with intimin, T3SS, and causes attaching and effacing lesions in large bowel, has shiga toxin

Question 14

Intimin

Answer 14

Adhesin

Question 15

Shiga Toxin

Answer 15

Causes hemorrhagic diarrhea and kidney failure

Question 16

STEC

Answer 16

Shiga toxin producing E coli = EHEC

Question 17

VTEC

Answer 17

Verocytotoxin producing E coli = EHEC

Question 18

Cattle EHEC Reservoir

Answer 18

Do not have shiga toxin receptor allowing cells to internalize toxin, infected cows are healthy

Question 19

Edema of Pigs

Answer 19

EHEC causes edema of eyelids, muscle tremor, squealing and snoring, and flaccid paralysis from shiga toxin in pigs

Question 20

EIEC

Answer 20

Enteroinvasive E coli, non flagellated, enters and replicates in macrophages in colon, destroys colonic epithelium, causes watery to bloody diarrhea, animals resistant

Question 21

ExPEC

Answer 21

Extraintestinal pathogenic E coli, septicemic, avian pathogenic, uropathogenic, and mammary pathogenic E coli

Question 22

SEPEC

Answer 22

Septicemic E coli, invades bloodstream in newborns or immune compromised, has serum resistance, iron uptake system, fimbriae, LPS, and cytotoxic necrotizing factors

Question 23

Cytotoxic Necrotizing Factors

Answer 23

Induces strong inflammatory response and leads to shock

Question 24

SEPEC Clinical Signs

Answer 24

Pyrexia, depression, weakness, arthritis in calves, watery mouth and death in lambs, and pneumonia in horse dog and cat

Question 25

APEC

Answer 25

Avian pathogenic E coli, causes systemic disease of birds, type 1 and P fimbriae, LPS, serum resistance, and aerobactin iron uptake

Question 26

UPEC

Answer 26

Uropathogenic E coli, causes UTI, forms intracellular biofilm, type 1 pili, hemolysin, aerobactin,a nd capsule

Question 27

UPEC in Dog

Answer 27

Cystitis, pyelonephritis, pyometra, prostatitis, females are more susceptible because bacteria enter urethra

Question 28

MPEC

Answer 28

Mastitis from fecal contamination and relaxation of teat sphincter after milking

Question 29

Mastitis Causes

Answer 29

E coli, Strep uberis, Staph auerus

Question 30

Diagnosis

Answer 30

Hemolytic on blood agar, lactose fermenter on MacConkey, metallic on EMB agar, dark blue on chromogenic

Question 31

IMViC Test

Answer 31

Positive indole, positive methyl red, negative voges-proskauer, negative citrate

Question 32

Next Generation Sequencing

Answer 32

Multilocus sequence typing, Pulsed-field gel electrophoresis, randomly amplified polymorphic DNA

Question 33

ETEC Diagnosis

Answer 33

Enterotoxin ELISA, virulence genes, latex agglutination for fimbrial antigens

Question 34

EPEC Diagnosis

Answer 34

Positive urease test, virulence gene, mucosal effacement on histopath

Question 35

STEC Diagnosis

Answer 35

Clinical findings, hemolytic, serotypes, virulence genes

Question 36

SEPEC Diagnosis

Answer 36

Isolation from blood, colicin V plasmids

Question 37

Colicin V Plasmids

Answer 37

Confer serum resistance

Question 38

UPEC DIAgnosis

Answer 38

Isolation from urine, type 1 pili

Question 39

Treatment

Answer 39

Rehydration, antibiotics