Immunology: Humoral Immune Responses II Flashcards
B cells that interact strongly with soluble self antigens becomes
Anergic and cannot again respond to that self antigen
B cell development is driven by
Antigen
On binding to antigen, B cells undergo
Isotype switching and somatic mutation
Types of cells that can make monoclonal antibodies
- Primed B cells
- Fusion partner
- Hybridomas
Which cells can grow in HAT medium (Are not defective in nucleotide synthesis)
Primed B cells and Hybridomas
Which cells cannot grow in HAT medium (Have defective nucleotide synthesis)
Fusion partner
Which cells can grow almost indefinitely in culture
Hbydridomas and Fusion partner
Which cells cannot grow indefinitely in tissue culture
Primed B cells
Which cells can secrete Ab of interest
Prime B cells and Hybridomas
Which cells cannot secrete Ab
Fusion Partner
___ are valuable therapeutic agents when conjugated because of their small size, which allows for ____
- Sc Fv molecules
- small size = ready tissue penetration
Diagnostic test with monoclonal antibodies
- Agglutination
- Precipitation
- Enzyme-linked immunosorbent assay (ELISA)
- Immunohistochemistry
- Immunoblotting (Western Blotting)
- Fluorescent-activated cell sorting (FACS)
Agglutination purpose
Determine ABO blood groups of blood donors and transfusion recipients
Purpose of enzyme-linked immunosorbent assay (ELISA)
HIV diagnosis
Purpose of immunohistochemistry
Diagnostic pathology
