Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

French And Norwegian > 3bio10 > Flashcards

3bio10 Flashcards

1
Q

Define biotechnology

A

The use of living organisms of some industrial purpose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is modern biotechnology used for

A

Genetic modification techniques

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Other name for recombinant DNA technology

A

Genetic engineering

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Define genetic engineering

A

Insertion of a desirable gene into an organism for some industrial purpose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What are restriction enzymes

A

Enzymes that cleave DNA at certain sequences

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Where are most restriction enzymes taken from

A

Bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Sticky vs blunt ends

A

Sticky ends have overhanging strands and blunt ends don’t

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are vectors

A

Things that carry the gene of interest and have the site for the restriction enzyme and also genes that help with cloning

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Example of a vector

A

Plasmid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Steps for cloning

A

Isolate the desired gene, choose a vector, cut the vector with the same restriction enzyme as for the target gene and ligate them

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Whats the product of gene cloning called

A

Recombinant DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What do you put into a clone

A

Recombinant DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is bacterial transformation

A

Intake of the recombinant plasmid into a cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Whats the template in PCR

A

The DNA helix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What seperates DNA helix in PCR

A

Heat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What makes DNA daughter strands in PCR

A

DNA polymerase

17
Q

What does the reaction mixture contain at the first cycle

A

DNA helix, four different types of dntps, DNA polymerase and two different types of primers

18
Q

What are four types of dntps needed for PCR

A

Deoxyadenine triphosphate, deoxycytosine triphosphate, deoxythymine triphosphate and deoxyguanine triphosphate

19
Q

What must be true about the DNA polymerase used in PCR

A

It is stable at high temperatures

20
Q

Where to get DNA polymerase used in PCR

A

Thermophilic bacteria

French And Norwegian (80 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions