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Flashcards in Arbo 3 Deck (51):
1

Reoviridae genus

Coltivirus: Colorado Tick Fever Virus

2

Coltivirus structure

  • 12 Double stranded RNA segments
  • NON enevloped 
  • icosahedral

3

Coltivirus replication cycle (5 steps)

  1. endocytosed, enters lysosome
  2. core particle primary transcript
  3. Translated into +/- strands in cytoplasm
  4. replicase creates + strand from the - one
  5. capsid assembly and release 

4

Colorado tick fever is a ____, _____ illness

nonspecific, febrile

5

Coltivirus clinical findings (4)

  1. Tick bite
  2. Saddleback Fever
  3. Flu-like symptoms
  4. Transient Rash

(Complication of Encephalitis or pericarditis)

6

Coltivirus infects _______ cells.

 

What effect does this have?

Bone Marrow cells

 

this halts development of blood cell types (PMN, eos, baso's)

7

When do Coltivirus symptoms occur

4-5 days after tick bite

8

Significance of the first two weeks of Coltivirus infection

free virus can be found in the blood

after which it circulates within erythroblasts

9

When does Coltivirus antibody appear?

two weeks after symptom onset

10

Coltivirus lifespan in blood

can be isolated for 6 weeks

BUT can live in RBC for life of the cell

11

Coltivirus resevoir and transmitter

Squirrel/rodent resevoir

Wood Tick is transmitter

12

What tick spreads Coltivirus?

Rocky Mountain wood tick

(Dermacentor andersoni)

13

90% of Coltivirus cases happen during...

april-july

14

Coltivirus control

stay away from ticks

check yo pets

get out of your skin if embedded

15

Filoviridae diseases

Ebola

Marburg

16

Characteristics that filoviridae share

 

Genome structure

Zoonotic

Damage the microvasculature = hemorrhage

17

Filovirus organism structure

ss (-) RNA

Encodes 7 proteins

Enveloped filaments

Helical

18

Filovirus nucleocapsid enclosed in an envelope that contains...

one glycoprotein

19

Filovirus replicates in the

cytoplasm

20

Filovirus (ebola) causes ______ infection

hemorrhagic

21

Ebola rash appearance and time

macropapular, appears at 5 days

22

Signs of recovery occur at day ____ and are concurrent with...

Day 7

With antibody response

23

Ebola transmission and reservoir

Unknown

possibly bats

24

Major characteristic of Ebola (filo) infection is...

destruction of the immune system

especially the breakdown of endothelial cells

25

Ebola cell damage due to ____

Cytokine release from Monocytes

26

Ebola cytokine dysregulation promotes ______ and ______

hemorrhage + vasomotor collapse

27

Ebola vector

 

Not identified

28

Rotavirus serogroups

 

A-E

29

Rotavirus genome and structure

nonenveloped

dsRNA

3 capsid layers + inner core

(Outer capsid VP4)

30

Rotavirus VP4 protein is located on _____ and functions to aid _______

 

Outer capsid

host attachment

31

Is rotavirus segmented or not?

Yes

11 segments of dsRNA

32

Rotavirus transcription and translation occur in _______

Inner core = transcription

Cytoplasm = translation

 

33

What is different about the replication of Rotavirus?

replicates in progeny core

uses positive RNA template

 

34

Rotavirus release from cell, two steps...

 

buds into ER

release by cell lysis

35

Rhabdovirus genus

Lyssavirus

36

Rhabdo shape and structure

bullet shaped helical, enveloped

ss (-) RNA

37

Rhabdo: associated proteins

NPMGL

 

(nucleo, phospho, matrix, glyco, polymerase)

38

Rhabdo replication

  1. Binds using G protein
  2. Pinocytosis into cytoplasm
  3. Endosome fusion and release RNP into cytoplasm
  4. L gene (pol-ase) makes 5 mRNAs + 1 full (+)RNA
  5. Translation of NPMGL (G = processed in ER and Golgi)
  6. +RNA serves as templates for full length (-) RNA
  7. Assemble NPL around RNP core, M forms capsule (matrix)
  8. Bud from PM and release

39

Rhabdo periods (and # of days for each)

 

Incubation (20-90)

Prodromal (2-10)

Neurological (2-7)

40

Rhabdo travels via..

retrograde axonal transport

41

Rhabdo pathogenesis duration depends on...

size & site of exposure

human/animal immunity

42

Rhabdo replicates in _____

muscle at the site of innoculation

43

Rhabdo concentrates in ______

 

salivary glands

44

common reservoirs for rhabdo

bats, foxes, raccoons, skunks

45

Rhabdo Dx

Histopathology

(also electron micro,

direct elisa/immunochemistry)

46

signs of rhabdo in histopathology are... (5)

 

  1. mononuclear infiltration
  2. perivascular cuffing (lymph/PMN)
  3. Lymphocytic foci
  4. Babes nodules
  5. NEGRI BODIES

47

Rhabdo vaccine is unique in that it is adminitered

Post exposure

48

Other measures taken after exposure to rhabdo (other than post exposure vax)

Clean site with soap and water

49

Rhabdo vax administration schedule after exposure?

 

Days - 0, 3, 7, 14, 28

50

Is there a pre exposure rhabdo vaccine?

 

Yes

3 doses

51

What is the Wisconsin rabies treatment protocol?

Ketamine

Ribavirin

Amantadine