Lecture 4 - Resp Viruses Flashcards Preview

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Flashcards in Lecture 4 - Resp Viruses Deck (85):
1

Adenovirus is only virus with ______

A fiber in each of its vertices

2

The _______fiber is ______ to cell

adenovirus, toxic

3

Adenovirus genome, capsid shape, and envelope

dsDNA, icosahedral, NONenveloped

4

Adenovirus contains ___on penton base

Hemagglutinin

5

Adenovirus genome contains _______.

Inverted terminal repititions

6

Adenovirus replication occurs in the _____

nucleus

7

Adenovirus has the same receptor as _____

Coxsackie B (CAR)

8

Adenovirus enters cell via ______. This happens due to....

ENDOCYTOSIS, due to interaction between PENTON BASE and AV integrins

9

Adenovirus transcription is a ______ phase event. What are they?

2 phase. (EARLY and LATE)

10

Adenovirus early phase is before ______

DNA replication

11

Adenovirus uses ____ to make mRNA

host RNA polymerase

12

Early adenovirus mRNA's =

"E" - 1a, 1b, 2, 3, 4

13

Adeno Intermediate Early genes =

E1a and E1b. Their products are needed for transcription of other delayed genes (E2-4)

14

All early genes are involved in... (3 processes)

DNA synthesis, Altering host expression, Transcription of LATE GENES

15

Early proteins (from early genes) in adenovirus help block ________

CTL response

16

Adeno Clinical Presentations (5)

ACUTE RESP DISEASE, OCULAR DISEASE (Pharyngoconjunctival fever, Epidemic Keratoconjunctivitis), ACUTE HEM. CYSTITIS, GASTROENTERITIS, INFECTIONS

17

Acute respiratory diseases caused by which adenoviruses

1 2 5 6

18

Acute respiratory diseases are mostly _______

nonsymptomatic

19

Adeno transmission

sputum + oral secretion

20

Adeno Sx

Fever, rhinorrhea, cough, sore throat.

21

Adenovirus is major cause of ______

OTITIS MEDIA and tonsilitis

22

Adenovirus - related ARD widespread in what specific population? What serotypes?

Military recruits -- Serotype 4 and 7

23

Adenovirus "supercold" serotype

14

24

Pharyngoconjunctival fever Adenovirus strains and buzzwords

3 4 7, summercamps, pool fever

25

Adenovirus Pharyngoconjunctivits eventually...

spreads to the other eye

26

Adeno Epidemic keratoconjunctivitis strains

8 19 37

27

Adeno keratoconjunctivitis inubation

8 days

28

Adeno keratoconjunctivitis presents...

bilaterally

29

Adeno keratoconjunctivitis may result in

residual scarring and visual impairment

30

Adeno acute hemorrhagic cystitis serotypes

11 + 21

31

Adeno acute hemorrhagic cystitis Presentation

Dysuria, HEMATURIA

32

Adeno Gastroenteritis Serotypes

40 + 42

33

Common cause of infantile diarrhea in daycare?

Adenoviral Gastroenteritis

34

Adeno may cause infections in IC patients with..

Transplant, T cell depletion, AB therapy, Lymphopenia

35

Adeno infection in IC patients can cause _____ which is associated with ___________ in more than 90% if cases

Nephritis, Acute renal failure

36

Adeno can be transmitted via what (2) routes?

DROPLETS (respiratory) and FECAL-ORAL (GI infctn).... Also can be from hand to eye or from poorly Chlorinated pools

37

Adenovirus is capable of causing _____ or _____ infection

Lytic or Latent

38

Adeno Lytic infection infects ___, ____, and _____ cells, which causes _______

Mucoepithelial, Gastrointestinal, Conjunctival cells, causes CELL DAMAGE

39

Adeno causes ______ (in rats), in which viral DNA does what?

Oncogenic Transformation, virus DNA integrates in host DNA

40

Adenovirus is a _______ virus, so it is more resistant

naked

41

Adenovirus is most common in _____ and _______

children and military recruits

42

Adenovirus treatment, and vaccine?

No treatment -- LIVE ATTENUATED vaccine has been discontinued

43

Coronavirus enveloped or not?

Enveloped

44

Corona genome (type and segmented?)

ss+RNA, non segmented

45

Corona capsid shape

Helical

46

Corona envelope appearance

club shaped glycoproteins (SPIKE, ENVELOPE, MEMRANE proteins)

47

Corona "Spike" protein function

receptor binding and cell fusion

48

Corona "Membrane" protein structure and function

Transmembrane, for budding and envelope formation

49

Corona replicates in the ___

cytoplasm

50

Replication process for Coronavirus: Steps 1 and 2

S protein binds to Aminopeptidase N and a SIALIC ACID containing receptor..... Virus is ENDOCYTOSED

51

Replication process for Coronavirus: Steps 3 -5

3. Protein synthesis - Early and late phases. 4. Genome associates with rER membrane. 5. Vesicles migrate to surface and are released

52

Replication process for Coronavirus: Its uses what enzyme? What are it's products?

RNA dependent RNA polymerase. Produces full length NEGATIVE RNA template and SIX individual mRNA's for other Coronavirus proteins

53

Coronavirus causes ________ (generally)

common cold (URTI)

54

URTI from coronavirus is a _______ illness

self limited, contagious

55

Coronavirus replicates best at what temps?

33-35 degrees Celsius

56

Coronavirus pathogenesis: Infected cell appearance

Vacuolated, damaged cilia, syncitia

57

Viral load curve during coronavirus infection is followed by curve for __________

Interferon response

58

_______ is slowly produced after interferon response (in coronavirus infection)

Antibody

59

Coronavirus is _____% of URTI. Only ______ cause more.

10-15%. Rhinoviruses

60

Despite antibodies to coronavirus, _______ is possible

reinfection

61

_______ helps coronavirus survive the GI tract for ________ transmission

Glycoprotein "Corona", Fecal-oral

62

Coronavirus control

none

63

SARS transmitted via

respiratory droplets

64

SARS begins with

high fever and PNA in most cases

65

SARS Sx (5)

Fever, Ache, dry cough, PNA, Diarrhea in 10-20%

66

SARS causes _________ by which three methods?

Tissue damage caused by 1. Direct LYTIC effects 2. Syncitial GIANT cells 3. Indirect IMMUNE RESPONSE

67

Coronavirus found in areas with lots of ______

animal species

68

SARS was introduced to humans how? How do we know this?

From ANIMALS. No Antibodies in people who did not have SARS

69

Picornavirus genera that have relevance to humans?

Entero, Rhino, Hep A

70

Rhinovirus have ______ serotypes that exhibit

over 100 serotypes, no geographic pattern

71

Rhinovirus Genome

ss+RNA

72

Rhinovirus Capsid shape

Icosahedral (small)

73

Rhinovirus enveloped or not?

No envelope (naked)

74

Rhinovirus replicates in the

cytoplasm

75

Rhinovirus RNA translated into ______

polyprotein that becomes ENZYMATIC and STRUCTURAL proteins

76

Most rhinoviruses are _______

cytolytic

77

Which are more common with rhinoviruses, upper RTIs or Lower RTIs

URTIs

78

First symptom of rhinovirus

Nasal irritation

79

____ is largely absent in rhinovirus infection

Fever

80

Complications of rhinoviruses

Acute Otitis Media (bacterial), and Exacerbation of Asthma

81

Rhinovirus has a __% infection rate for those exposed

95% (75% become ill)

82

Viruses inefficient at ____ when temp's reach _____

Replication, 33'C

83

Rhinovirus can not survive in the _______

GI tract

84

Incubation time for rhinovirus is

8-10 hrs

85

Cause of rhinovirus mucosal symptoms is due to

Immune response (IFN gamma and IL-8 stimulate a CYTOKINE cascade)