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Flashcards in Retro Deck (26):
1

Retrovirus structure

large, enveloped

ss (+)RNA

 

2

Retrovirus is the only virus to contain _______

diploid RNA genome

3

Retrovirus enzymes

RT

integrase

protease

4

3 Retrovirus genera that infect humans

Lentivirus

Oncovirus (HTLV)

Spumavirus

5

Lentivirus properties (3)

Bodily fluid transmission

non-transforming

have CD4+ T cell tropism

6

HIV believed to be native to

Monkeys of W Africa

7

HIV genome structure and contents

2 identical +RNA strands consisting of 3 major genes

  1. Gag
  2. Pol
  3. Env

8

HIV gag protein function

capsid and matrix

9

HIV Pol function

polymerase

core contains RT, integrase, and protease

10

HIV Env function

Envelope (glycoproteins)

11

HIV glycoproteins, both encoded by ENV gene, are ___ and ___.

gp120 (attachment)

gp41 (fusion)

12

HIV genome is +RNA, BUT....

it is NOT infectious

(because it does not encode a polymerase that directly generates more mRNA)

13

HIV attachment and binding (4) steps...

 

  1. gp120 -> CD4
  2. CCR5 (CXCR4) recruitment
  3. gp41 inserts into membrane
  4. fusion

 

14

Steps following HIV attachement (5)

Initial copies of viral DNA made, (2) dsDNA inserted into the host cell chromosome and the host cell RNA POL is used to make viral RNA. (3) New RNA strands serve as templates and as mRNA. (4) mRNA translated to viral proteins to make envelope. (5) New viral particles bud off

15

HIV: primary stage features

short flu-like illness

(OR asymptomatic)

Infecter person CAN infect others

16

Stage 2 HIV - Asymptomatic phase

Average 10 years

No Sx (maybe swollen glands)

HIV drops to very low levels

**HIV Antibodies are detectable in blood**

17

Stage 3 HIV - Symptomatic Phase

Mild symptoms and opportunistic infections

18

Stage 4 HIV

Progression to AIDS

19

Bacterial, Viral, and Fungal oppurtinistic infections

B = TB, Strep pneumo

V= Kaposi Sarcoma, Herpes, Influenza

F= Candida. P. carinii, cryptococcus

20

AIDS defining disease mentioned my Dr. Mohammed

Pneumocystis carinii pneumonia

21

Early --> late opportunistic infection (Catergories)

 

bacterial,

VZV,

Candida, PCP,

CMV, MAC,

Fungi (TOXO!)

22

3 AIDS definitions

  1. CD4 under 200
  2. CD4 below 14%
  3. HIV+ AND co-infection with opportunistic agent (PCP, toxo, MAC, KS, etc...) REGARDLESS of T cell count

23

Most CD4 cells are killed during the _____ period of HIV

incubation

24

What is a blood analysis going to look like in an full blown AIDS patient?

High HIV RNA + Antigen

Low CD4 and NK (eventually)

 

25

HIV drug classes (3)

  1. Nucleoside RT inhibitors
  2. Non-Nucleoside RT inhibitors
  3. Protease inhibitors

26

Non-Nucleoside RT inhibitors are only for _______

HIV-1