B and T Cell Maturation

Question 1

TF GATA-3 promotes development of what cells?

Answer 1

TH2

Question 2

Name the stages of progression for B cell development?

Answer 2

early pro-B, late Pro-B, large pre-B, small pre-B, immature, transitional 1, transitional 2, mature

Question 2

What is one disadvantage of having TI mediated B cell activation without T helper involvement?

Answer 2

no memory

Question 3

What does c-Kit do?

Answer 3

regulates replication

Question 4

Double negative (DN) 2 thymocytes are positive for these genes?

Answer 4

c-Kit, CD 44 and 25

Question 4

Which transcription factor promotes development of TH1?

Answer 4

T-BET

Question 6

What cytokines are required for B cell differentiation?

Answer 6

IL-3, 4 and LBCGF (low molecular weight B cell growth factor)

Question 7

What is the life-span for a naive B cell?

Answer 7

days to weeks

Question 7

Define anergy in context of T cell development

Answer 7

a T cell is not costimulated by B7 and is not activated

Question 9

Where does negative selection occur for B cells?

Answer 9

bone marrow

Question 9

Pre-B lymphocytes have this receptor to promote proliferation and division?

Answer 9

IL-7R

Question 10

If a thymocyte is positive for CD 44, what instruction is it receiving?

Answer 10

to relocate to the thymus

Question 11

All double negative thymocytes express this gene?

Answer 11

c-Kit

Question 12

Double negative thymocytes 2,3,and 4 (T cells DN2, DN3, and DN4) are found in what location?

Answer 12

sub-scapular cortex of thymus

Question 13

What are some TH2 cytokines that promote B cell division? That promote B cell differentiation?

Answer 13

division= IL-4 differentiation= IL-4, 5, 6, 10, 13

Question 14

Where do you find B1 cells?

Answer 14

peritoneum and pleural cavities

Question 15

Low serum Ig memory cells, class switch recombination and B cell activation are all symptoms of what deficiency and what is the cause?

Answer 15

common variable immunodeficiency, mutation in CD 40, 19 BCR, or a co-stimulating molecule

Question 16

Binding of what protein to the BR3 receptor of a B cell in transitional phase promotes what?

Answer 16

protein= Blys promotes=survival

Question 17

Describe a super-antigen. Where does it bind? Where is it found?

Answer 17

binds CD28 , found in staphylococcal infections (food poisoning)

Question 19

What markers and immunoglobins would you expect to find on a B2/follicular cell?

Answer 19

CD 19/21, 23, IgM and IgD

Question 21

B cells an be activated without T helper involvement via these antigens? (name two types)

Answer 21

T independent (TI) 1 = bacterial cell wall components like LPS (binds TLR4) T independent (TI) 2= large polysaccharides like flagellin or viruses that bind BCR

Question 22

A mutation in the Bruton tyrosine kinase results in what deficiency?

Answer 22

agammaglobulinemia = immunodeficiency XLA

Question 23

Double negative thymocyte 4 (DN4) mature to what T cell stage?

Answer 23

DP (double positive) T cells

Question 24

How often do B cells in the marginal zone of the spleen self-renew?

Answer 24

long-lived (no self renewal necessary)

Question 25

Compare the lag-time of a memory B cell to that of a naive B cell.

Answer 25

memory= 1-3 days naive= 4-7 days