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Boards.CP.Micro > Bacteriology-Methods > Flashcards

Bacteriology-Methods Flashcards

1
Q

What bacteria grow best at 25-30C? (2)

A
  • Yersinia entercolitica

- Psuedomas spp.

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2
Q

Gram Stain Morphology:

-Pyogenic streptoccoci

A

Gram Positive Cocci

-Pairs/Short chains

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3
Q

Indole Test use/function.

A

Identification of E. coli (indole Positive)

*not unique to E. coli

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4
Q

What biochemical test is used to identify Group B pyogenic strep and Listeria monocytogenes?

A

CAMP Test

*Both produce CAMP factor

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5
Q

Gram Stain - Gram Negative features:

  • Peptidoglycan*
  • Outer membrane

*Staining characteristic

A
  • Thin peptidoglycan cell wall*
  • Outer membrane present

*Lose crystal violet/Iodine during decoloarization – Visualized w/ Red Safranin counterstain

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6
Q

Hippurate Hydrolysis Test is used for the presumptive identification of which organisms? (5)

A
  • Campylobacter jejuni
  • LIsteria monocytogenes
  • Strep agalactiae
  • Gardnerella vaginalis
  • Legionella pneumophila

*All are POSITIVE

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7
Q

Oxidase Positive Organisms (7)

A
  • Neisseria spp.
  • Moraxella
  • Pseudomonas
  • Legionella
  • Campylobacter*
  • Vibrio*
  • Helicobacter*

*Curved Gram Negative RODS

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8
Q

Gram Stain Morphology:

-Campylobacter

A

Gram Negative Rods

  • Thin, Curved
  • S-shaped, Seagull/Corkscrew
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9
Q

Oxidase Negative Organism.

A

Enterobacteriaceae

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10
Q

What selective media is used for recovery of Neisseria spp. from nonsterile sites?

A

Thayer-Martin medium

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11
Q

Which organisms don’t grow on Sheep blood agar? (3)

A
  • N. gonorrhea
  • H. influenzae
  • Legionella
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12
Q

Gram Stain Morphology:

-Staphylococci

A

Gram Positive Cocci

-Grapelike clusters

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13
Q

Novobiocin Susceptibility testUse/Function.

A

S. saprophyticus (Novobiacin Resistent) Vs. other coagulase Negative staphylococci
-S. epidermidis (Novobiacin Susceptible)

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14
Q

What selective media is used for recovery of Yersinia spp.?

A

Cefsulodin-Irgasan-Novobiocin agar

CIN

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15
Q

Growth in Lysozyme containing broth medium is indicative of what bacteria?

A

Nocardia

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16
Q

Oxidase Test use/function.

A

Initial classification of Gram Negatives

17
Q

What medium is used to recover Legionella spp.?

A

Buffered Charcoal Yeast Extract agar

BCYE

18
Q

Coagulase Test Use/Function.

A

S. aureus (Coagulase Positive) Vs. other Staphylococci (Coagulase Negative)

*should always be confirmed using the tube test

19
Q

Gram Stain Morphology:

-Vibrio spp.

A

Gram Negative Rods

  • Curved, Comma-shaped
  • Short rods
20
Q

Gram Stain Morphology:

-Yersinia pestis

A

Gram Negative Rods

-Bipolar staining (“Closed Safety-Pin”)

21
Q

Gram Stain Morphology:

-Nonpyogenic streptococci

A

Gram Positive Cocci

-Long chains

22
Q

Gram Stain - Gram Positive features:

  • Peptidoglycan
  • Outer membrane

*Staining characteristic

A
  • THICK peptidoglycan cell wall*
  • NO outer membrane

*Retains purple crystal violet/Iodine stain during the decolarization step (alcohol)

23
Q

Catalase Test Use/Function.

A

Gram Positive Cocci

  • Staphylococci - Catalase Positive
  • Streptococci/Enterococci - Catalase Negative
24
Q

Why don’t Mycobacteria stain well with Gram Stain?

A

Mycolic Acids in Peptidoglycan Cell wall

-Hydrophobic-Repels Crystal violet/Iodine

25
Q

PYR tests use/function.

A

Identifies Group A pyogenic streptococci and Enterococcus spp.

(Positive Test - Red color change)

26
Q

Which body sites normally harbor indigenous Anaerobic flora (and should not be cultured for anearobes)? (5)

A
  • Stool
  • Skin
  • Oropharynx
  • Vagina
  • Urethra
27
Q

What selective media inhibits growth of Gram Positive bacteria and many Enterics?
-What does grow?

A

Selenite Broth

-Samonella

28
Q

Gram Stain Morphology:

-Neisseria spp.

A

Gram Negative Diplocci

-Coffee Bean shaped

29
Q

Listeria monocytogenes - Temperature characteristics.

A

37C - Optimal growth

25C - Shows motility

4C - Able to multiply

30
Q

Optochin Susceptibility test (P disk) Use/Function.

A

S. pneumoniae (Optocin Susceptible*) vs other alpha-hemolytic Streptococci
-Viridans strep (Optocin Resistent)

*Shows Clearing

31
Q

Besides Staphylococci, what other bacteria are Catalase Positive? (3)

A
  • Campylobacter spp.
  • Listeria spp.
  • Bacillus spp.
32
Q

Gram Stain Morphology:

-S. pneumoniae

A

Gram Positive Diplocci

-Lancet shaped

33
Q

Acid-Fast Stain principle.

A

Uses Heat and/or Phenol to allow a Fuchsin dye to penetrate the Hydrophobic barrier
-Stains Mycolic Acids in cell wall

34
Q

Gram Stain Morphology:

-Enterococcus

A

Gram Positive Diplocci

35
Q

Bile Esculin Test Use/Function.

A

Identifies Enterococci and Group D strep (S. bovis)

36
Q

What selective media is used for recovery of Vibrio spp.?

A

Thiosulfate-Citrate-Bile Salts-Sucrose agar

TCBS

37
Q

Rapid Urease Test use/function.

A

Proteus spp. and H. pylori identification
-Urease Positive

(Red/Pink coloration)

38
Q

Gram Stain Morphology:

-Legionella

A

Gram invisible in primary smear

39
Q

Campylobacter spp. grows best at what temperature?

A

42C

Boards.CP.Micro (28 decks)

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