HSV,CMV,EBV,HHV6,HHV8,Adeno,Parvo,JC/BK,HPV,Pox

Question 1

Viral culture Cytopathic Effect (CPE):

-Tear shaped cells

Answer 1

Enterovirus

Question 2

Viral culture Cytopathic Effect (CPE):

-CMV

Answer 2

Focal plaques in HDF

Question 3

Viral culture Cytopathic Effect (CPE):

-Grape-like clusters

Answer 3

Adenovirus

Question 4

Viral culture Cytopathic Effect (CPE):

-RSV

Answer 4

Syncytial cells

Question 5

Viral culture Cytopathic Effect (CPE):

-HSV

Answer 5

Sweeping, globular cells

Question 6

A positive IgG antibody may indicate what?

Answer 6

Current or Past infection

Question 7

A positive IgM antibody indicates what?

Answer 7

Current infection

Question 8

Viral Histology - RSV:

• Nuclear/Cytoplasmic Inclusions
• Syncytia (Y/N)
• Notes

Answer 8

• NONE

- Yes

Question 9

Viral Histology - HSV :

• Nuclear/Cytoplasmic Inclusions
• Syncytia (Y/N)

-Notes (Cowdry type “?”, 3Ms)

Answer 9

• Nuclear
• Yes
• Cowdry type A
• 3Ms (Multinucleated, Molded nuclei, Marginated chromatin)

Question 10

Viral Histology - Adenovirus:

• Nuclear/Cytoplasmic Inclusions
• Syncytia (Y/N)

-Notes

Answer 10

• Nuclear
• No

-“Smudge Cells”

Question 11

Viral Histology - CMV:

• Nuclear/Cytoplasmic Inclusions
• Syncytia (Y/N)

-Notes

Answer 11

• Nuclear&Cytoplasmic
• No

-“Owl eye” inclusion, NOT multinucleated

Question 12

Viral Histology - Measles:

• Nuclear/Cytoplasmic Inclusions
• Syncytia (Y/N)

-Notes

Answer 12

• Nuclear&Cytoplasmic
• Yes

-“Warthin-Finkeldey Giant cells”

Question 13

Viral Histology - Rabies:

• Nuclear/Cytoplasmic Inclusions
• Syncytia (Y/N)
• Notes

Answer 13

• Cytoplasmic
• No

-Negri bodies

Question 14

Where does HSV type I go dormant?

Answer 14

Trigeminal Ganglia

Question 15

Where does HSV type 2 go dormant?

Answer 15

Sacral Gangia

Question 16

HSV definitive dx method.

Answer 16

Culture

-CPE within 2-3 days

Question 17

Neonatal CMV features.

Answer 17

• Intracerebral calcifications
• Petechial Rash (coag)
• microcephaly

Question 18

EBV pathogenesis:

Answer 18

Pharyngeal or genital mucosa

-Infects B-lymphs via C3d receptor (CD21)

Question 19

What type of lymphocyte is seen in p. smear of EBV. (Atypical lymphocytosis)

Answer 19

-CD8+ T lymphocytes

Question 20

What is the site of EBV latency?

Answer 20

B-lymphocytes

Question 21

Uncontrolled activation of T/NK cells following EBV infection, leading to fuliminant primary EBC, that is frequently fatal

Answer 21

Duncan disease  (X linked immunoproliferative disease)
-SH2D1A (SAP) gene

Question 22

What is the monospot test based upon?

Answer 22

Frequent presence of heterophile antibodies in EBV infection

Question 23

IHC stain that performs nearly as well as EBER.

Answer 23

-LMP1

Question 24

Virus responsible for a significant proprotion of child hood febrile seizures (highly neurotropic; may cause encephalitis)

Answer 24

-HHV6

Question 25

HHV8 associations. (3)

Answer 25

- Kaposi sarcoma - Primary Effusion Lymphoma - Kastleman's Dz in HIV+

Question 26

Adenovirus serotypes in most respiratory infections.

Answer 26

1-14 & 21

Question 27

Adenovirus serotypes ass. w/ hemorrhagic cystitis

Answer 27

11 & 21

Question 28

Adenovirus serotypes ass. w/ childhood gastritis

Answer 28

40 & 41

Question 29

Virus associated with Aplastic Anemia.

Answer 29

Parvovirus B19 (Erythrovirus)

Question 30

Where do JC & BK viruses lay dormant?

Answer 30

Urothelium and Lymphocytes

Question 31

Reactivation of JC virus features.

Answer 31

- PML - Infects Oligos - AIDS (MC setting) - Smudgy nuclear inclusions in oligos

Question 32

Reactivation of BK virus features.

Answer 32

- Hemorrhagic cystitis and/or - Polyomavirus nephropathy - Transplant (MC setting) - Decoy cells - smudgy nuclear inclusions

Question 33

``` HPV type (order of frequency): -Common wart ```

Answer 33

2, 1, 4

Question 34

``` HPV type (order of frequency): -Genital warts (condyloma acuminatum) ```

Answer 34

6, 11

Question 35

``` HPV type (order of frequency): -LSIL ```

Answer 35

6, 11

Question 36

``` HPV type (order of frequency): -HSIL ```

Answer 36

16, 18, 31, 33, 35

Question 37

``` HPV type (order of frequency): -AIS/Cervical Adenoca ```

Answer 37

18, 16, 45