Gram Negative Cocci

Question 1

Neisseria spp. - Diagnosis:

• Incubation environment
• Oxidase
• Gram stain
• Medium (3)

Answer 1

-Fastidious, require incubation in CO2 rich environment
-Oxidase Positive
-Intracelluar Gram Neg. diplococci (Coffee Bean)
Chocolate agar w/ antibiotics:
-Thayer-Martin
-Martin-Lewis
-New York City

Question 2

N. gonorheae:

• % Asymptomatic (M/F)
• Disseminated infection leads to?

Answer 2

Asymptomatic:

• Males 10%
• Females 50%

Fitz Hugh Curtis syndrome or Purulent Arthritis

Question 3

N. meningitides:

• Diagnosis
• U.S.A Serogroups
• Colonization location
• Invasive infections

Answer 3

• Identified by culture
• Serogroups B*, C, and Y
• Nasopharyngeal carriage (10%)
• Bacterial meningitis

Question 4

Moraxella catarrhalis:

• Gram stain
• Oxidase
• Blood agar characteristic
• Colonization location
• Causes what infections (2)

Answer 4

• Intracelluar Gram Neg. diplococci (Coffee Bean) (similar to Neisseria)
• Oxidase Positive
• Hockey Puck sign on blood agar
• Upper Respiratory Tract
• Otitis media (kids); COPD exacerbation (adults)

Question 5

Kingella kingae:

• Gram stain
• Hemolysis
• Infections (4; Adult/Kids)

Answer 5

-Gram Neg. coccobaccillus; pairs/short chains
-Beta hemolytic
-Endocarditis (adults)
Young Kids (most commonly)
-Septic arthritis
-Osteomyelitis
-Occult bacteremia