Block 3 - Lec 28

Question 1

an alternative form of a given gene

Answer 1

allele

Question 2

recombinant dna of mixed parentage, from chimera

Answer 2

chimeric dna

Question 3

creating multiple copies of a single dna construct

Answer 3

cloning

Question 4

ss end of ds dna often generated by cutting with a restriction enzyme

Answer 4

sitcky end

Question 5

independently replicating dna useful for cloning

Answer 5

vector

Question 6

gel electrophoresis can be used to differentiate by these

Answer 6

size, complexity, amount of rna, amount of dna, amount of protein

Question 7

this is used to detect the location of radioactive materials like in a gel

Answer 7

autoradiography

Question 8

duplex dna sequences that are cut by restriction enzymes are frequently blank

Answer 8

palindromes

Question 9

this is used to identify the size and amount of specific dna fragments using either radioactive rna or dna molecules as probes

Answer 9

southern transfer

Question 10

this is used to identify the size and mount of specific rna molecules using radioactive dna molecules as a probe

Answer 10

northern transfer

Question 11

EcoRI is a restriction enzyme that creates a blank overhang

Answer 11

5’

Question 12

SstI is a restriction enzyme that creates a blank overhang

Answer 12

3’

Question 13

PvuII is a restriction enzyme that creates a blank end

Answer 13

blunt

Question 14

this is used to identify the size and mount of specific protein molecules using radioactive antibodies as a probe

Answer 14

western blotting

Question 15

a small ds dna used to attach sticky ends to a blunt ds dna

Answer 15

adaptor

Question 16

dna with one cleavage site that is a ring used in cloning

Answer 16

plasmid

Question 17

ways to link dna

Answer 17

cohesive ends, blunt end ligation, linkers, tailing

Question 18

rna can be used to create this kind of dna and make copies for cloning

Answer 18

cDNA

Question 19

this is used to digest the hook to leave the double stranded dna

Answer 19

S1

Question 20

each bacteriophage holds about blank base pairs between ends

Answer 20

15k

Question 21

Talens and CRISPR are used with guide blank to find specific sequences and make double strand breaks to blank them for gene modification

Answer 21

rna, remove

Question 22

this is used to amplify dna of a known sequence

Answer 22

pcr

Question 23

humans differ by about blank percent in dna sequence according to CNV

Answer 23

.5%

Question 24

short repeating units of dna

Answer 24

STR

Question 25

y chromosome is passed down from blank to blank

Answer 25

father, son

Question 26

insulin can be created in long acting and short acting forms for blank

Answer 26

diabetes

Question 27

rATP has a blank and blank hydroxyl groups

Answer 27

2', 3'

Question 28

dATP has a blank hydroxyl

Answer 28

3'

Question 29

ddATP has a blank hydroxyl

Answer 29

none

Question 30

this terminates making dna

Answer 30

ddATP

Question 31

classical reading a sequence from gel electrophoresis

Answer 31

singer method

Question 32

prototypical vector that has several restriction sites with cohesive ends to clone into present as one site

Answer 32

pBR322

Question 33

PstI is blank resistant

Answer 33

ampicillin

Question 34

BamHI and SalI are blank resistant

Answer 34

tetracycline

Question 35

plasmids are blank can be used for cloning

Answer 35

bacteriophages

Question 36

yac cloning uses a blank for cloning

Answer 36

plasmid

Question 37

cosmids have a mix of blank and blank dna

Answer 37

phage, plasmid