Lec 7 Kinetics

Question 1

k is the blank

Answer 1

rate constant

Question 2

enzymes speed up attainment of equilibrium by influencing blank

Answer 2

k

Question 3

linear portion of equilibrium curve is what we use to determine blank

Answer 3

rate

Question 4

enzyme assay when product yield is measured throughout entire reaction

Answer 4

continuous assay

Question 5

assay where individual time points are taken

Answer 5

sampled assays

Question 6

assay where two metabolic reactions that share a common intermediate … product of first is substrate of the second

Answer 6

coupled assay

Question 7

these can enhance sensitivity, and facilitate data collection during assay

Answer 7

synthetic substrate

Question 8

enzyme is saturated with substrate at blank

Answer 8

Vmax

Question 9

study of how enzymes influence the rates of change

Answer 9

enzyme kinetics

Question 10

Km =

Answer 10

[S] at 1/2 Vmax (Affinity of enzyme for substrate)

Question 11

Km and Vmax are part of this model

Answer 11

Michaelis menten

Question 12

michaelis menten model is a blank

Answer 12

square hyperbola

Question 13

Km does not equal

Answer 13

Vmax / 2 because it is the substrate concentration at 1/2 Vmax

Question 14

an enzyme does not have to obey michaelis menten and can have a blank

Answer 14

Km

Question 15

an enzyme that acts on more than one substrate it will usually have a different blank for each

Answer 15

Km

Question 16

low Km means blank affinity of E for S

Answer 16

high

Question 17

high Km means blank affinity of the E for S

Answer 17

low

Question 18

a good way to tell isozymes apart is to look at each of their….

Answer 18

Km’s

Question 19

low affinity (high Km) may mean that the enzyme isnt even ….

Answer 19

catalyzing the reaction

Question 20

hexokinase and glucokinase are blank

Answer 20

isozymes

Question 21

glucokinase is found mostly in the blank and blank

Answer 21

liver/beta cells of pancreas

Question 22

hexokinase as a blank affinity for glucose than glucokinase

Answer 22

higher

Question 23

hexokinase is present in blank

Answer 23

most tissues including liver

Question 24

glucokinase responds when the levels of glucose are blank

Answer 24

high

Question 25

hexokinase functions more during blank levels of glucose

Answer 25

normal

Question 26

inhibitors work in a variety of ways and differ in terms of their blank

Answer 26

specificity

Question 27

general inhibitors like heavy metals inactivate enzymes blank and blank

Answer 27

irreversibly, non specifically

Question 28

specific enzyme inhibitors are useful in medicine because they can mimic the blank to target enzyme

Answer 28

substrate

Question 29

these bind at the active center and rom a permanent covalent bond to the enzyme

Answer 29

irreverisble inhibitors

Question 30

two types of reversible inhibitors

Answer 30

competitive, non competitive

Question 31

competitive inhibitors bind to the blank center by non covalent bonds

Answer 31

active

Question 32

noncompetitive inhibitors do not bind to blank

Answer 32

active site

Question 33

competitive inhibitors are influenced by amount of blank but blank are not

Answer 33

substrate, noncompetitive

Question 34

competitive inhibitor blank Vmax and blank Km

Answer 34

does not change, increases

Question 35

noncompetitive inhibitor blank Vmax and blank Km

Answer 35

decreases, doesnt change

Question 36

converting hyperbolic function of michaelis menten model into linear

Answer 36

lineweaver burk

Question 37

competitive makes a blank lineweaver burk plot

Answer 37

x intercept change

Question 38

noncompetitive lineweaver burk plot changes blank

Answer 38

y intercept

Question 39

an enzyme who's activity is influenced by multiple variables

Answer 39

allosteric

Question 40

hemoglobin is not an enzyme but has blank properties

Answer 40

allosteric

Question 41

enzymes regulated by allosteric effects always have more than one subunit and more than one center....aka

Answer 41

quaternary structure

Question 42

enzyme that adds phosphate onto a side chain

Answer 42

kinase

Question 43

enzyme that breaks off a phosphate group

Answer 43

phosphatase

Question 44

adding a phosphate to something like serine or threonine would make it look more like blank because of negative charge

Answer 44

aspartic acid/glutamic acid

Question 45

active digestive proteases cannot be synthesized inside cells because they would blank the cells that made them

Answer 45

damage

Question 46

proteases are secreted as inactive precursors... aka

Answer 46

zymogens

Question 47

zymogens are blank in the gut

Answer 47

activated

Question 48

enteropeptidase is synthesized in cells lining the blank and secreted directly.... even though it is an active protease it is no danger to cells that secrete it because it has such a blank

Answer 48

duodenum, limited specificity

Question 49

kinetic studies amount of product formed in amount of time formula

Answer 49

deltaP/deltaT = k[S]