Flashcards in Blood Transfusion Deck (15):
What is the minimum weight you have to be to give blood?
What infections is donated blood tested for?
HIV (antibody +PCR)
HCV (antibody +PCR)
HBV (antibody +PCR)
What are the important points in storage of red cells?
- 35 day shelf life
- If removed for more that 30 mins must be either transfused or discarded.
- Must be transfused within 4 hours of leaving storage
What are the important points in storage of platelets?
Stored at 22 degrees with continual agitation
Shelf life of 5 days
Transfuse within 1 hour
What are the important points in storage of fresh frozen plasma?
Stored at -30 degress for up to 2 years.
Thawed prior to transfusion and transfused within 4 hours
What chromosome is blood type (ABO) coded on?
What chromosome is rheus blood type coded on?
Why would you have irregular red cell antibodies such as anti kell?
These are not naturally occurring like anti A and Anti B and develop after previous exposure to red cells eg from transfusion or pregnancy
What test do you use for testing for allo antibodies?
Indirect antiglobulin test
Which antibodies mediate an acute immediate haemolytic reaction caused by ABO incompatibility?
Why is an ABO incompatible transfusion so fast to occur? How does the reaction occur?
- IgM antibodies forma Membrane Attack complex which causes full activation of the complement cascade
- C3a and C5a are released which causes vessel dilation, histamine release and increases vascular permeability.
- MAC leads to rupture of transfused red cells.
- Thromboplastic materal from haemolysed red cells leads to indiscriminate activation of the coagulation mechanism and DIC
- Formation of bradykinin causes arteriolar dilation and increased vascular permeability which leads to hypotension.
How long after a transfusion does a delayed haemolytic reaction occur?
5 - 10 days
What antibodies mediate an urticarial reactions to a blood transfusion?
What are the most common causitive bacteria in a bacterial transfusion reaction with red cells?