Chapter 2: Types of Microscopes Flashcards Preview

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Flashcards in Chapter 2: Types of Microscopes Deck (8)
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Bright-field Microscope

Forms its image when light is transmitted through the specimen; produces image that is darker than the surrounding illuminated field.


Dark-field Microscope

Puts a "block" on a bright-field microscope that blocks all light from entering the objective lens except peripheral light; creates image of brightly illuminated specimens surrounded by a dark field


Phase-Contrast Microscope

Transforms subtle changes in light waves passing through specimen into differences in light intensity (different parts of specimen have different densities); best for internal detail


Interference Microscope

Manipulates light like phase-contrast but adds contrasting colors to the image and two beams of light rather than a single one; produces extremely well-defined images that are vividly colored and appear 3-D


Fluorescent Microscope

Modified compound microscope with an Ultraviolet radiation source and a filter that protects the viewer's eye; specimen must be coated with a source of fluorescence


Confocal Microscope

Overcomes problem of cells or structures being too thick causing microscopes to have trouble focusing; Uses a laser beam of light to scan various depths in the specimen; captures highly focused view at any level


Transmission Electron Microscope

Produces image by transmitting electrons through the specimen; specimens must be sectioned in extremely thin slices and stained or coated with metals


Scanning Electron Microscope

Bombards surface of a whole metal-coated specimen with electrons while scanning back and forth over it; electron pattern is displayed on a TV screen; color is added afterwards