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3rd year > CULTIVATION > Flashcards

CULTIVATION Flashcards

(88 cards)

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1
Q

PRINCIPLE OF CULTIVATION

A
  • To grow and isolate all bacteria present in a clinical specimen
  • To determine which of the bacteria that grow are most likely contaminants or colonizers.
  • To obtain sufficient growth of clinically relevant bacteria to allow identification and characterization
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2
Q

Process of growing microorganisms in culture by taking bacteria from the infection site

A

CULTIVATION

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3
Q

bacteria with relatively complex, and exceptional media components must be used.

A

Fastidious

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4
Q

made from seaweed

A

AGAR

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5
Q

made by acid or enzymatic hydrolysis of animal or vegetable protein, namely muscle, liver, blood, milk, casein. Lactalbumin, gelatin or soybean

A

PEPTONE

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6
Q

baker’s yeast

A

YEAST EXTRACT

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7
Q

contain soluble organic bases, protein degradation, products, vitamin and minerals

A

MEAT EXTRACT

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8
Q

agarose-containing medium

A

AGAR

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9
Q

petri dish containing the agar

A

AGAR PLATE

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10
Q

resulting bacterial population derived from single bacterial cell

A

COLONY

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11
Q

bacterial cultures derived from a single colony

A

PURE CULTURE

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12
Q

MEDIA CLASSIFICATION

A
  • Enrichment
  • Supportive
  • Selective
  • Differential
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13
Q

Contain specific nutrients required for the growth of bacterial pathogens

A

ENRICHED MEDIUM (ENRICHMENT)

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14
Q

Example of enriched media

A

Buffered charcoal yeast extract agar- Legionella pneumophilia

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15
Q

Contain nutrients that support the growth of most nonfastidious organisms without giving any particular organism a growth advantage.

A

SUPPORTIVE MEDIA

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16
Q

Contain one or more agents that are inhibitory to all organisms except those being sought.

A

SELECTIVE MEDIA

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17
Q

Example of inhibitory agents

A
  • Dyes
  • Bile salts
  • Alcohols
  • Acids
  • Antibiotics
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18
Q

Has added inhibitors that discourage the growth of certain organisms without inhibiting the growth of organism being sought.

A

SELECTIVE MEDIUM

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19
Q

inhibit Gram ( + ) bacteria

A

MacConkey agar

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20
Q

selective for Gram ( + ) bacteria

A

PEA and CAN

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21
Q

selective for Neisseria gonorrhea.

A

THAYER MARTIN MEDIUM, MARTIN LEWIS AGAR, NEW YORK CITY AGAR

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22
Q

salt tolerant haloduric bacteria ( S. aureus ).

A

MANNITOL SALT AGAR

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23
Q

Allows colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria

A

DIFFERENTIAL MEDIA

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24
Q

Permits differentiation of organisms that grow on the medium.

A

DIFFERENTIAL MEDIUM

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25
based on the organisms ability to grow in 40% bile and to hydrolyze esculin to produce esculitin
BILE ESCULIN AGAR
26
Esculin reacts with _____ to form a brown black precipitate.
FERRIC CITRATE
27
growth indicates tolerance to 40% bile(40% oxygall) blackening indicates hydrolysis of esculin
POSITIVE
28
lack of growth indicates inability to grow in 40% bile lack of color change indicates inability to hydrolyze esculin
NEGATIVE
29
Bile esculin agar A. Positive -
Entercoccus faecalis
30
B. Negative -
Streptococcus viridans
31
Cultivation of non-fastidious organisms
BLOOD AGAR PLATE
32
Determination of hemolytic reaction
BLOOD AGAR PLATE
33
Enrichment for Legionella spp.
Buffered Charcoal Yeast Extract (BYCE)
34
The medium consists of a base containing
protein source, soybean protein, digest (containing a slight amount of natural carbohydrate), sodium chloride, agar, and 5% sheep blood
35
Certain bacteria produce extracellular enzymes that lyse red blood cells in the agar
HEMOLYSIS
36
Complete clearing of the red blood cells around the bacterial colony
BETA HEMOLYSIS
37
Only partial lysis of the cells to produce a greenish discoloration around the colony
ALPHA HEMOLYSIS
38
Other bacteria have no effect on the red blood cells, and no halo is produced around the colony
GAMMA OR NON HEMOLYTIC
39
Potato Glycerol Based Medium
BORDET GENGOU AGAR
40
% defribrinated blood
15-20
41
inhibitor of bordet gengou agar
Methicilin
42
Isolation of Bordetella pertussis
Bordet Gengou Agar
43
BYCE is supplemented with
polymixin B, and ansamycin
44
Contains vancomycin, trimethoprim, polymixin, amphotericin B, and cephalothin
Campy blood agar
45
Selective for Camphylobacter
Campy blood agar
46
Selective medium for Camphylobacter spp.
Cefoperazone, Vacomycin, Amphotericin (CVA) medium
47
- Selective medium for Yersinia species - Useful for the isolation of Aeromonas
Cefsulodin-ingasan Novobiocin Agar
48
is essentially the same as blood agar except that during preparation the red blood cells are lysed when added to molten agar base.
Chocolate agar
49
(“X” factor)
hemoglobin, hemin
50
nicotinamide adenine dinucleotide
(NAD or “V” factor)
51
components of chocolate agar
- Peptone base - solution of 2% hemoglobin or Isovitalex
52
The most common bacterial pathogens that require this enriched medium for growth include
Neisseria gonorrheae, Haemophilus spp.
53
Selective isolation of gram positive
Columbia Colistin –Nalidixic agar
54
Infusion agar base with 5% sheep blood
Cystine Tellurite Blood agar
55
Reduction of potassium tellurite by Corynebacterium diptheriae
Cystine Tellurite Blood agar
56
Isolation of C. diptheriae
Cystine Tellurite Blood agar
57
Peptone base with lactose and sucrose.
Eosin Methylene Blue Agar (EMB)
58
Isolation and differentiation of lactose fermenting (LF) and non- lactose fermenting (NLF) enteric bacilli
Eosin Methylene Blue Agar (EMB)
59
is used for the cultivation of gastro-intestinal pathogens (i.e.,Salmonella spp. and Shigella spp.) from stool specimens and rectal swabs.
Gram negative broth (GN)
60
Gram negative broth also contains _______ as the primary carbon source.
mannitol
61
contains bile salts and dyes (bromthymol blue and acid fuchsin) to selectively slow the growth of most nonpathogenic gram- negative bacilli found in the gastrointestinal tract and allow Salmonella spp. And Shigella spp. to grow.
Hektoen Enteric (HE)Agar
62
The medium is also differential because many non-enteric pathogens that do grow will appear as
orange to salmon- colored colonies.
63
This colony appearance results from the organism’s ability to ferment the lactose in the medium, resulting in the production of acid, which lowers the medium’s pH and causes a change in the pH indicator bromthymol blue
Hektoen Enteric (HE)Agar
64
Peptone base with lactose.
MacConkey Agar
65
Gram + inhibited by
crystal violet and bile salts
66
Indicator of MacConkey agar
Neutral red
67
Isolation and differentiation of LF from NLF
MacConkey Agar
68
Modification of MacConkey agar in which lactose has been replaced with d-sorbitol as the primary carbohydrate source
MacConkey Sorbitol Agar
69
For the selection and differentiation of E.coli 0157:H7 in stool specimens
MacConkey Sorbitol Agar
70
Peptone base with mannitol
Mannitol Salt Agar (MSA)
71
indicator or MSA
phenol red
72
Selective for isolation of staphylococci
Mannitol Salt Agar (MSA)
73
% NaCl (MSA)
7.5
74
Peptone base with cornstarch, supplemented with yeast dialysate, 3% hemoglobin, and horse plasma
New York City (NYC) Agar
75
Selective for Neisseria gonorrhea
New York City (NYC) Agar
76
is essentially sheep blood agar that is suplemented with phenylethyl alcohol to inhibit the growth of gram negative bacteria
Phenylethyl alcohol (PEA)
77
Charcoal agar supplemented with horse blood, cephalexin, and amphotericin B
Regan Lowe
78
Enrichment and Selective for medium for the isolation of Bordetella pertussis
Regan Lowe
79
Peptone base with lactose, ferric citrate, and sodium citrate
Salmonella-Shigella Agar
80
indicator of Salmonella-Shigella Agar
neutral red
81
Peptone and soy protein base with yeast extract, dextrose and buffer
Schaedler Agar
82
Nonselective medium for the recovery of anaerobes and aerobes
Schaedler Agar
83
Peptone base broth
Selenite broth
84
Enrichment for isolation of Salmonella
Selenite broth
85
Peptone and soy protein base with lysed horse blood
Skirrow agar
86
Selective for Campylobacter spp.
Skirrow agar
87
Contains crystal violet, colistin, trimethoprim sulfamethoxazolee in 5% sheep blood
Streptococcal Selective Agar
88
Selective for Streptococcus pyogenes and Streptococcus agalactiae
Streptococcal Selective Agar

3rd year (35 decks)

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