HEMA LABBB

Question 1

measures the number of red blood cells in a volume of blood

Answer 1

RBC count

Question 2

Normal Values
Male:
Female:
Newborn:

Answer 2

4.2-5.4 x 1012/L
3.6-5.0 x 1012/L
5.5-6.5 x 1012/L

Question 3

Helps differentiate types of anemia
(microcytic, macrocytic)

Answer 3

Anemia Evaluation

Question 4

Identifies increased RBC mass

Answer 4

Polycythemia Screening

Question 5

Evaluates the effectiveness of
therapies like erythropoietin

Answer 5

Monitoring treatments

Question 6

Provides a reference method to validate automated counts.

Answer 6

Manual Counting

Question 7

The space between the top of the platform
and cover glass is

Answer 7

0.1mm

Question 8

Each large square is

Answer 8

1mm wide and 1 mm long

Question 9

the entire ruled area is

Answer 9

9mm2

Question 10

The volume of one entire platform is

Answer 10

0.9uL

Question 11

What type of pipette is used for RBC counting

Answer 11

Thoma RBC pipet (1:200)

Question 12

RBC diluting fluid

Answer 12

a. Trisodium citrate with formalin
b. Hayem’s solution
c.Isotonic saline, isotonic Gower’s solution and Citrate formalin solution

Question 13

causes roleaux and agglutination in patients with hyperglobulinemia

Answer 13

Hayem’s solution

Question 14

COMPUTATION FOR RBC COUNT

Answer 14

No. of cells counted x Correction for Dilution x Correction for Volumex106

Question 15

Dilution

Answer 15

200

Question 16

Correction for Volume

Answer 16

50

Question 17

Specimen for peripheral blood smear

Answer 17

EDTA-anticoagulated whole blood
Free-flowing capillary blood

Question 18

The most commonly used stain in the hematology laboratory

Answer 18

Romanowsky-type stain

Question 19

is defined as any stain containing methylene blue and/or its products of oxidation and a halogenated fluorescein dye, usually eosin B or Y

Answer 19

Romanowsky stain

Question 20

are alcoholic solutions with basic and acidic components.

Answer 20

Wright, Giemsa, or May-Grünwald stains

Question 21

Counting Method

Answer 21

1. cross sectional method
2. longitudinal method
3. battlement method

Question 22

Examine the RBC morphology

Answer 22

• Presence of inclusions and abnormalities
• Presence of immature RBCs
• Size and pallor of RBCs
• Rouleaux and agglutination
• Polychromatophilia

Question 23

Immature granulocytic cells

Answer 23

shift to the left

Question 24

Hypersegmented neutrophils

Answer 24

shift to the right

Question 25

is an iron containing protein molecule found in red blood cells which transport oxygen from the lungs to the body’s tissues.

Answer 25

Hemoglobin

Question 26

Hemoglobin A1

Answer 26

96-98% It contains two alpha subunits and two beta subunits.

Question 27

Hemoglobin A2

Answer 27

1.5-3.2% It contains two alpha subunits and two gamma subunits

Question 28

Hemoglobin F

Answer 28

0.5-0.8% It contains two alpha subunits and two delta subunits. 

Question 29

Decreased Hemoglobin

Answer 29

Anemia

Question 30

Increased Hemoglobin

Answer 30

Possible polycythemia

Question 31

Preferred for standard measurement of hemoglobin due to accuracy and precision of result.

Answer 31

Automated

Question 32

counter check the result released by the machine, or if there is no machine available in the area or laboratory.

Answer 32

Manual Testing

Question 33

Direct/Visual Colorimetry

Answer 33

ACID HEMATIN TECHNIQUE

Question 34

Hemoglobin is converted to Acid Hematin by mixing blood with

Answer 34

0.1 N Hydrochloric acid

Question 35

Photoelectric / Light Absorbance Method

Answer 35

CYANMETHEMOGLOBIN METHOD (HiCN)

Question 36

DRABKIN’S SOLUTION (pH 7.0 – 7.4)

Answer 36

Potassium cyanide 50 mg Potassium ferricyanide 200 mg Sodium bicarbonate 1 g Distilled water 1 liter

Question 37

Blood is lysed with the use of

Answer 37

non-ionic detergents

Question 38

is the reference method for Hb estimation

Answer 38

cyanmethemoglobin method

Question 39

All Hb forms except ______ is estimated

Answer 39

sulphaemoglobin

Question 40

Another reason for turbidity is lipemic specimen. To correct this, add

Answer 40

0.1 ml of Patient’s plasma to 5ml of reagent and use the solution blank.

Question 41

are used to correct abnormal globulins.

Answer 41

Addition of potassium carbonate

Question 42

can be resistant to hemolysis and cause turbidity.

Answer 42

Hb S and Hb C

Question 43

NORMAL RANGES Adult Males: Adult Females: Newborns and Infants:

Answer 43

13-17 g/dl 11.5-16.5 g/dl 14-22g/dl

Question 44

Carboxyhemoglobin =

Answer 44

hemoglobin + carbon monoxide

Question 45

hemoglobin in which ferrous iron are oxidize to ferric state.

Answer 45

Methemoglobin

Question 46

irreversible formation of hemoglobin from sulphonamides and aromatic amines.

Answer 46

Sulfhemoglobin

Question 47

is the volume of RBCs expressed as a percentage of the volume of whole blood in a sample

Answer 47

Hematocrit

Question 48

This test measures the proportion of the RBC to plasma in peripheral blood but not in the entire circulation

Answer 48

Hematocrit

Question 49

Decreased hematocrit

Answer 49

Anemia

Question 50

Increased hematocrit

Answer 50

Dehydration (Relative) Polycythemia vera (Absolute)

Question 51

Not commonly used today since it is time consuming, requires larger amounts of blood, and generally contains a higher percentage of trapped plasma

Answer 51

MACROHEMATOCRIT METHOD

Question 52

with red band Used for non-anticoagulated samples

Answer 52

Heparinized

Question 53

With blue band Used for anticoagulated samples

Answer 53

Plain

Question 54

Capillary Tube Details

Answer 54

Approximately 75 mm long with Approximately 1.2 mm inner bore