Diagnostic Microbiology Flashcards Preview

PH 152 > Diagnostic Microbiology > Flashcards

Flashcards in Diagnostic Microbiology Deck (29):
0

Set of strains within particular species

Type

1

True or false. Establishing maximum inhibitory conc is part of susceptibility testing of isolate

False.minimum, not maximum

2

Basis of classical methods of bacterial separation

Metabolic & Morphological characteristics

3

Basis of modern taxonomic approach

Bacteria's structural composition

4

Comparison of species does not involve comparison of multiple strains for each species. True or false?

False

5

Hybridization and sequencing are process of __analysis

Molecular

6

What is DNA-DNA homology?

How well 2 diff DNA strands hybridize

7

Purpose of DNA-DNA homology

To test genetic relatedness

8

Sequencing of ___ : "gold standard" in bacterial taxonomy

16S rRNA

9

16S rRNA provides complementary info for DNA-DNA homology. True or false?

True

10

If sample has no prior culture, how can the antigen be detected?

Aggregation (to antibody-coated latex beads)

11

Process used to amplify bacterial DNA from human fluid

Polymerase chain reaction

12

Serologic test can be done even before infection. True or false?

False. Several weeks after dapat

13

Sequence the five I's
A. Inspection
B. Incubation
C. Isolation
D. Identification
E. Inoculation

E B C A D

14

True or false. No microbes require live organism as inoculation media

False. Some do (animal/egg)

15

Identify:
* catalase positive
* Gram positive in chains
* strictly aerobic

Micrococcus

16

All are common bet Neisseria, Moraxella and Branhamella except
a) Gram negative cocci
b) positive in both oxidase and catalase
c) facultative anaerobe

c) facultative anaerobe

aerobic kasi

17

Identify:
* catalase negative
* anaerobic
* oxidase negative
* strictly aerobic

Veilonella

18

All are common bet Bacillus and Clostridia except
a. sporeformer
b. gram + rod
c. aerobic

c. aerobic

anaerobes ang Clostridia

19

All are common bet Mycobacterium and Nocardia except
a. acid-fast
b. forms spores
c. rods

b. forms spores

20

common traits of Listeria and Lactobacillus

gram positive, non-sporeforming rods

21

common traits of Corynebacterium and Propionibactrium

gram positive, pleomorphic, nonsporeforming rods

22

Sequence the acid fast stain procedure
a. methylene blue
b. carbol fuschin
c. acid alcohol

B C A

23

semi-solid media is often used for __, __

demonstration of motility, anaerobe isolation

24

Identify mismatched pair of selective media
a. MacConkey: gram positive
b. Sabouraud: fungi
c. Lowenstein Jensen - Mycobacteria
d. MSA - Staph

a. MacConkey: gram positive

negative dapat

25

differentiate capsule from slime layer

mas defined ang capsule

26

True or false: capsules are not lost during in vitro culture

false

27

Wall-less bacteria that cannot replicate but have outer membrane

spheroplasts

28

wall-less bacteria that cannot replicate and do not have outer memebrane

protoplasts