DNA Enveloped (EBV,KSHV, CMV, HHV6, HHV7)

Question 1

what is the biology of EBV?

Answer 1

envelped double stranded DNA

Question 2

what cells does EBV target for primary infection?

Answer 2

muco-epithelial and B cells

Question 3

what cells does EBV select to remain latent and for secondary infection?

Answer 3

B cells

Question 4

what does EBV use to immortalize b cells?

Answer 4

EBNA (epstein bar nuclear antigen)

Question 5

how do you treat EBV?

Answer 5

no vaccine, no treatment

Question 6

how do you diagnose EBV?

Answer 6

fever

hepato-splenomegaly

swollen lymp nodes

white patches in the tonsils

macular rash

Question 7

how do you confirm a diagnosis of EBV?

Answer 7

Atypical Downey cells

Question 8

how do you know if an infection of EBV is current or re-current?

Answer 8

current = anti-VCA IgM

recurrent = anti-EBNA IgG

Question 9

what is this?

Answer 9

Atypical Downey cell in EBV

Question 10

what non-histological test can be used to confirm diagnosis of EBV?

Answer 10

heterophile monospot test

Question 11

what complications can occur with EBV?

Answer 11

1) Ora hairy leukoplakia
2) Hodgkins lymphoma
3) Burkitt’s lymphoma
4) nasopharyngeal lymphoma

Question 12

how is EBV transmitted?

Answer 12

by saliva

Question 13

when is EBV mostly seen?

Answer 13

as infectious mononucleosis in college era (15-20’s)

Question 14

what is needed to contain an infection of EBV? and what is the consequence?

Answer 14

cell mediated response is needed but in will cause damage to our cells leading to inflammation

Question 15

why does EBV cause sore throat?

Answer 15

because of the destruction of epithelial cells

Question 16

why do atypical downey cells appear?

Answer 16

due to T cell activation and proliferation having atypical appearance

Question 17

infectious mononucleosis is another name for what?

Answer 17

EBV

Question 18

infectious mononucleosis macular rash is seen in what part of the body?

Answer 18

from the trunk to head and forearms

Question 19

what do you not give to infectious mononucleosis patients with macular rash?

Answer 19

antibiotics because it exacerbates the rash (hypersensitivity)

Question 20

when EBV infects B cells, what gets secreted?

Answer 20

heterophile antibodies

Question 21

what test can be used to confirm presence of heterophile antibodies?

Answer 21

monospot test

Question 22

monospot test can differentiate between what 2 viruses?

Answer 22

CMV or EBV

Question 23

anti-EBNA is indicative of what?

Answer 23

past infection and not active infection

Question 24

what is anti-VCA a definitive of?

Answer 24

definitive infection of EBV

Question 25

what is this?

Answer 25

oral hairy leukoplakia

Question 26

what is this?

Answer 26

hodgkins lymphoma

Question 27

what is this?

Answer 27

burkitts lymphoma

Question 28

in what patients is Kaposi sarcoma most common?

Answer 28

AIDS patients

Question 29

HHV-8 is what?

Answer 29

kaposi sarcoma

Question 30

what symptom is mostly seen in kaposi sarcoma?

Answer 30

Red or violet or salmon pink to silver grey macules or nodules

Question 31

what does the kaposi sarcoma virus lytic infection do?

Answer 31

it releases viral proteins, activate the vascular G protein causes angiogenesis and tumor formation

Question 32

what is this? what virus?

Answer 32

red papules in kaposi sarcoma, HHV-8

Question 33

how is kaposi sarcoma treated?

Answer 33

no treatment, no vaccine, no control

Question 34

what is the biology of cytomegalovirus?

Answer 34

it is a enveloped double stranded DNA virus

Question 35

how is CMV transmitted?

Answer 35

mom to fetus transplants body fluids

Question 36

what cells does CMV target?

Answer 36

mononuclear cells like T cells or macrophages

Question 37

what cell does CMV target to remain latent?

Answer 37

Hematopoetic stem cells

Question 38

what is the treatment for CMV?

Answer 38

Gancyclovir

Question 39

what is the clinical presentation of CMV?

Answer 39

fever swollen lymph nodes hepato-splenomegaly

Question 40

how do you confirm a diagnosis of CMV?

Answer 40

histological owl eye intracnuclear inclusion

Question 41

what is this?

Answer 41

owl eye intranuclear inclusion

Question 42

what complications can occur with CMV?

Answer 42

cytomegalo-inclusion disease keratitis

Question 43

which are normal, which are CMV?

Answer 43

CMV are huge cells

Question 44

what cell will CMV impair?

Answer 44

T cells

Question 45

do you have heterophile immune cells with CMV?

Answer 45

no, B cells are unaffected

Question 46

what is curious about CMV?

Answer 46

we all get it and it remains latent

Question 47

what can trigger CMV activation?

Answer 47

immuno-compromised individuals infection pregnancy

Question 48

when is cytomegalo-inclusion disease most commonly seen?

Answer 48

neonates, because they lack cell mediated immunity

Question 49

when does CMV cause multisite symptomatic disease?

Answer 49

in transplant or AIDS patients

Question 50

how does CMV evade the hosts defense mechanism?

Answer 50

Blocks NK recognition Blocks NK activation Blocks TAP (MHC Class I) Encodes IL-10 analog thus preventing Th1 activity

Question 51

what is the most common in-utero infection in the US?

Answer 51

CMV

Question 52

what symptoms are seen in neonates with CID from CMV?

Answer 52

petachiae jaundice hepato-splenomegaly thrombocytopenia (blue-berry muffin baby)

Question 53

what symptoms are seen in AIDS or transplant patients with CMV?

Answer 53

pneumonia gastritis/colitis retinitis

Question 54

how is CMV detected?

Answer 54

direct immuno-florescence

Question 55

what does HHV-6 cause?

Answer 55

roseola infantum

Question 56

what is HHV-7?

Answer 56

exanthem subitum

Question 57

who is susceptible to develop roseola or exanthem subitum?

Answer 57

kids less than 2 y/o

Question 58

what is the treatment for HHV-6/7?

Answer 58

none, it goes away alone

Question 59

what is the clinical presentation for HHV-6/7?

Answer 59

sudden high fever and lacy rose-red maculo-papular rash 2 after fever goes away