DNA Enveloped (EBV,KSHV, CMV, HHV6, HHV7) Flashcards Preview

Microbiology Block 1 > DNA Enveloped (EBV,KSHV, CMV, HHV6, HHV7) > Flashcards

Flashcards in DNA Enveloped (EBV,KSHV, CMV, HHV6, HHV7) Deck (59):
1

what is the biology of EBV?

envelped double stranded DNA

2

what cells does EBV target for primary infection?

muco-epithelial and B cells

3

what cells does EBV select to remain latent and for secondary infection?

B cells

4

what does EBV use to immortalize b cells?

EBNA (epstein bar nuclear antigen)

 

5

how do you treat EBV?

no vaccine, no treatment

6

how do you diagnose EBV?

fever

hepato-splenomegaly

swollen lymp nodes

white patches in the tonsils

macular rash

7

how do you confirm a diagnosis of EBV?

Atypical Downey cells

 

8

how do you know if an infection of EBV is current or re-current?

current = anti-VCA IgM

recurrent = anti-EBNA IgG

9

what is this?

Q image thumb

Atypical Downey cell in EBV

10

what non-histological test can be used to confirm diagnosis of EBV?

heterophile monospot test

11

what complications can occur with EBV?

1) Ora hairy leukoplakia

2) Hodgkins lymphoma

3) Burkitt's lymphoma

4) nasopharyngeal lymphoma

12

how is EBV transmitted?

by saliva

13

when is EBV mostly seen?

as infectious mononucleosis in college era (15-20's)

14

what is needed to contain an infection of EBV? and what is the consequence?

cell mediated response is needed but in will cause damage to our cells leading to inflammation

15

why does EBV cause sore throat?

because of the destruction of epithelial cells

16

why do atypical downey cells appear?

due to T cell activation and proliferation having atypical appearance

17

infectious mononucleosis is another name for what?

EBV

18

infectious mononucleosis macular rash is seen in what part of the body?

from the trunk to head and forearms

19

what do you not give to infectious mononucleosis patients with macular rash?

antibiotics because it exacerbates the rash (hypersensitivity)

20

when EBV infects B cells, what gets secreted?

heterophile antibodies

21

what test can be used to confirm presence of heterophile antibodies?

monospot test 

22

monospot test can differentiate between what 2 viruses?

CMV or EBV

23

anti-EBNA is indicative of what?

past infection and not active infection

24

what is anti-VCA a definitive of?

definitive infection of EBV

25

what is this?

Q image thumb

oral hairy leukoplakia 

26

what is this?

Q image thumb

hodgkins lymphoma

27

what is this?

Q image thumb

burkitts lymphoma

28

in what patients is Kaposi sarcoma most common?

AIDS patients

29

HHV-8 is what?

kaposi sarcoma

30

what symptom is mostly seen in kaposi sarcoma?

Red or violet or salmon pink to silver grey macules or nodules

31

what does the kaposi sarcoma virus lytic infection do?

it releases viral proteins, activate the vascular G protein   causes angiogenesis and tumor formation

32

what is this? what virus?

Q image thumb

red papules in kaposi sarcoma, HHV-8

33

how is kaposi sarcoma treated?

no treatment, no vaccine, no control 

34

what is the biology of cytomegalovirus?

it is a enveloped double stranded DNA virus

35

how is CMV transmitted? 

mom to fetus

transplants

body fluids

36

what cells does CMV target?

mononuclear cells like T cells or macrophages

37

what cell does CMV target to remain latent?

Hematopoetic stem cells

38

what is the treatment for CMV?

Gancyclovir

39

what is the clinical presentation of CMV?

fever

swollen lymph nodes

hepato-splenomegaly

40

how do you confirm a diagnosis of CMV?

histological owl eye intracnuclear inclusion 

41

what is this?

Q image thumb

owl eye intranuclear inclusion

42

what complications can occur with CMV?

cytomegalo-inclusion disease

keratitis

43

which are normal, which are CMV?

Q image thumb

CMV are huge cells

A image thumb
44

what cell will CMV impair?

T cells

45

do you have heterophile immune cells with CMV?

no,  B cells are unaffected

46

what is curious about CMV?

we all get it and it remains latent

47

what can trigger CMV activation?

immuno-compromised individuals

infection

pregnancy

 

48

when is cytomegalo-inclusion disease most commonly seen?

neonates, because they lack cell mediated immunity

49

when does CMV cause multisite symptomatic disease?

in transplant or AIDS patients

50

how does CMV evade the hosts defense mechanism?

Blocks NK recognition

Blocks NK activation

Blocks TAP (MHC Class I)

Encodes IL-10 analog thus preventing Th1 activity

51

what is the most common in-utero infection in the US?

CMV

52

what symptoms are seen in neonates with CID from CMV?

petachiae

jaundice

hepato-splenomegaly

thrombocytopenia (blue-berry muffin baby)

53

what symptoms are seen in AIDS or transplant patients with CMV?

pneumonia

gastritis/colitis

retinitis

54

how is CMV detected?

direct immuno-florescence 

55

what does HHV-6 cause?

roseola infantum

56

what is HHV-7?

exanthem subitum

57

who is susceptible to develop roseola or exanthem subitum?

kids less than 2 y/o

58

what is the treatment for HHV-6/7?

none, it goes away alone

59

what is the clinical presentation for HHV-6/7?

sudden high fever and lacy rose-red maculo-papular rash 2 after fever goes away