E2: L14 - Bcell Maturation

Question 1

B cell development beings in the __ __

Answer 1

bone marrow

Question 2

B cell development is completed in the tissue ___

Answer 2

periphery

Question 3

in b-cells, only __ selection required

Answer 3

negative

Question 4

B-cells have no need for ___ restriction

Answer 4

MHC

Question 5

B-cell development begins in the bone marrow with a hematopoietic __ cell

Answer 5

stem

Question 6

B-cells end up in the ___ to differentiate into transitional B cells to produce a mature B-cell

Answer 6

spleen

Question 7

B cells create a ___ response

Answer 7

humoral

Question 8

Pre-B cell and immature b-cells are in the ___ ___

Answer 8

bone marrow

Question 9

pre-b cells are committed to the __ ___ line

Answer 9

b-cell

Question 10

B-cells change up the genes, so produced receptors can be very ___.

Answer 10

diverse

Question 11

inmature b-cells undergo ___ of cell lines. (negative selection)

Answer 11

deletion

Question 12

negative selection: removes things that are strongly selecting for __-___

Answer 12

slef-antigens

Question 13

b-cell receptors are often Ig__ and Ig__ is added with increased cell maturity.

Answer 13

IgM, IgD

Question 14

when B-cells leave the bone marrow, they express what antibody

Answer 14

IgM

Question 15

___ cells provide support and growth factor to developing cells

Answer 15

stromal

Question 16

SCF stands for

Answer 16

stem cell factor

Question 17

stem cells activate into ___ progenitors that produce Pre-B cells

Answer 17

lymphoid

Question 18

___-b cell stage is when the cell is committed to becoming a B-cell.

Answer 18

pre

Question 19

from pre B cell to B cell is when it leaves the bone marrow to go to the ___

Answer 19

spleen

Question 20

B-cells can be ___ by bone marrow

Answer 20

retrained

Question 21

plasma cells are an ___ bcell

Answer 21

effector

Question 22

mature b-cells can also be called ___ b-cells

Answer 22

naive

Question 23

ID of B-cell stages are done by:
cell-surface markers, transcription factors, Ig gene rearrangements

Question 24

HSC -> CLP this looses the ability to go down the __ line

Answer 24

myeloid

Question 25

moving pro B to immature B cell is Interleukin ___

Answer 25

7

Question 26

Stromal cells in the bone marrow produce _____ which, upon binding to receptors expressed on developing B cells, stimulates progenitor cell survival, proliferation, and transcription of B cell associated genes. A: IL-7R B: STAT5 C: Ikaros D: PU.1 E: IL-7

Answer 26

E: IL-7

Question 27

setting up early b-cell for genetic recombination of Ig genes occurs in the ___

Answer 27

nucleus

Question 28

IL-7 is produced by s___cells

Answer 28

stromal

Question 29

IL-7 stimulates b-cells to become ___ b-cells

Answer 29

immature

Question 30

immature B-cell receptors include only the __ chains

Answer 30

heavy

Question 31

Immature B cells in the bone marrow are exquisitely sensitive to __ induction.

Answer 31

tolerance

Question 32

B-cell surface receptors are tested against self-___

Answer 32

antigens

Question 33

clonal deletion and receptor editing are termed ___ ___ as they occur in bone marrow.

Answer 33

central tolerance

Question 34

If a cell is too reactive to self-antigen in negative selection, not all are forced into ___

Answer 34

apoptosis

Question 35

B-cell surface receptors can be ___ to not be overly reactive to self-antigens

Answer 35

selected

Question 36

___, a state of nonresponsiveness to further stimuli

Answer 36

anergy

Question 37

b-cell leaving bone marrow only has ___ on surface (antigen)

Answer 37

IgM

Question 38

when a b-cell is in the spleen, they differentiate into transitional b-cells that are ___.

Answer 38

subsets

Question 39

b-cells go through the transition stages in the __ pulp of the spleen

Answer 39

white

Question 40

Mature, primary B-2 B-cells migrate from spleen to ____ follicles

Answer 40

lymphoid

Question 41

primary B-___ B-cells respond to antigens with T-cell help by producing antibodies

Answer 41

2

Question 42

B-2 cells half-life is approx. ___ months

Answer 42

4.5

Question 43

b-cells have variable region ___

Answer 43

diversity

Question 44

B1 b-cells are constantly regenerated outside __ ___

Answer 44

bone marrow

Question 45

B__ B-cells secrete antibodies even in absence of antigen stimulation

Answer 45

1

Question 46

B1 B-cells populate in __ and __ cavityies

Answer 46

pleural, peritoneal

Question 47

B_ B-cells have "natural antibodies"

Answer 47

1

Question 48

___ B-cells are specialized for blood-borne Ag recognition

Answer 48

MZ

Question 49

MZ-bcells have a higher ___ for self-antigens than the other subtypes of B-cells

Answer 49

affinity

Question 50

Which of these is NOT a characteristic of B-2 B cells? A: dependent on IL-7 B: highly diverse V region C: develop into memory cells D: found in peritoneal and pleural cavities E: form from HSCs in the bone marrow

Answer 50

D

Question 51

both B and T cell developmental pathways have gene ___

Answer 51

rearrangement

Question 52

both B and T cell developmental pathways have ___ selection against self-antigens

Answer 52

negative

Question 53

both B and T cell developmental pathways produce ___ of cells with discrete function

Answer 53

subsets

Question 54

differences in B and T cell developmental pathways is their location of maturation and screening B: thymus T: bone marrow and spleen

Question 55

does negative selection occur in both b and T cells or only in B cells?

Answer 55

only b cells

Question 56

_ cells require antigen prevention

Answer 56

T

Question 57

__ cells require T-cell help and secrete antibodies

Answer 57

B

Question 58

APC stands for ______ and is what stimulates a T cell

Answer 58

antigen presenting cells