Lab 6: Small and Large Animal Semen Collection (Kelleman, Pozor) Flashcards
extenders can lengthen lifespan of canine sperm up to
30+ days
how often should you change egg yolk in canine extender?
q10d
which is better: phase contrast or bright field microscopy for evaluating sperm?
phase contrast
movement of proximal droplet in dog sperm
moves more distal as sperm moves through epididymis, and is eventually shed
blood in dog semen can indicate:
prostate problems (benign prostate hypertrophy, prostatitis), rough handling –> broken blood vessels on penis
how does semen quality in electroejaculated bull sperm compare to sperm collected with artificial vagina?
contains more pre and post-sperm fluid because glands stimulated more
-number, quality, motility will be different
electroejaculation used on stallions?
rarely (not tolerated well)
how does normal bull ejaculate?
with one powerful thrust
Bos indicus breeds need faster/slower induction of electroejaculation
slower
measure scrotal width or circumference in stallions?
width
Colorado artificial vagina advantages/disadvantages
ad: holds temp (well insulated), stimulates glans
dis: heavy, must closely control temp of water that goes in (too hot will kill sperm), need to add additional plastic liner
Missouri artificial vaginal advantages/disad.
ad: lighter, liner already assembled for you, less direct stimulation of penis
dis: doesn’t stimulate glans (some stallions need this), not as well insulated
how does normal stallion ejaculate
5-7 thrusts followed by ejaculation. Will see flagging of the tail when he is ejaculating
how long does extended semen in horses last?
~48hrs (some may survive up to 5-7 days)
horse extender composed of
milk-based with sugars (glucose, lactose) and abx (gentomycin, penicillin, etc.)
