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Flashcards in microbiolgy flash Deck (67)
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Answers

2

Ionizing radiation

High enough energy to knock electron out of orbit. Ex. X-ray, UV is NOT

3

mole

6.02x10^23 particles

4

molar

6.02x10^23 particles dissolved to final volume of 1L

5

pH

is equal to -log [H+]

6

cofactor

May be needed for a functional catalytic site (Ex. Fe or Zn)

7

coenzyme

NAD+ or FAD (both are vitamin derivatives)

8

artifacts

loss of tissue

9

Metachromasia

Change in color of stain due to interaction with cellular or extracellular components

10

Periodic Acid Shift (PAS)

Stains glycoproteins

11

Haemotoxylin

Stains acids (or basophilic)(neg. charge) structures blue. Nucleus, RNA

12

Eosin

Stains basic (or acidophilic)(+ charge) structures red

13

Diffuse through membrane

Lipid soluble (uncharged) molecules. Ex. water

14

Pinocytosis

Cell is sampling enviroment, non-specific intake

15

Receptor mediated endocytosis

proteins bind and a vesicle then forms to bring components into cell

16

Phagocytosis

Pseudopodia extend and engulf item (Ex. Bacteria)

17

Cytoskeleton composition

Made of polymerized subunits (including soluble cytoskeleton subunits). Microtubules, microfilaments, intermediate filaments

18

Microtubules

Hollow, polar, (+) end grows/shrinks. Function: Forms tracks for ATPase, form cilia, flagella, centriole and mitotic spindles

19

Dynein

Move to (-) end of microtubule

20

Kinesine

Move toward (+) end of microtubule

21

Cilia

9+2 arrangment, made of microtubules

22

Centrioles

Mone pair per cell, each has 9 triplets of microtubules at right angles. Located in center of cell. Attach to mitotic spindles to help cell divide

23

Spindle MT

Spindle integrity and elongation

24

Kinetochore MT

chromosome seperation

25

Microfilaments

Assembled from actin, formsvery small polar, helical array, grow on (+), shrink on (-). Function: microvilli, cytokinesis

26

Intermediate filaments

Function: Desmosomes, hemidesmosomes, maintain tissue level integrity, Can be used to determine origin of tumor

27

Lysosome

Origin: ER/golgi. Fuse with endosomes, phagosomes and agred organelles. Mannose-6-P distinguishes. Proton pumps in membrane to lower pH

28

Peroxisome

Oxidation reactions for long chain FA, produces H2O2 (degraded by catalase). Origin: ER

29

Hemosiderin

Accumulation of heme

30

Protein syn. Finishes in cystol target to:

Cytosol, nucleus, mitochondria, peroxisomes, or exocytosis