Microscopy Flashcards
Cell Biology (18 cards)
What do microscopes allow us to see?
Things that we can’t see with the naked eye.
What type of microscopes use light and lenses to form an image?
Light microscopes.
What is the primary function of light microscopes?
To magnify specimens and allow us to see individual cells and large subcellular structures.
What do electron microscopes use to form an image?
Electrons.
True or False: Electron microscopes have a lower magnification than light microscopes.
False.
What is resolution in microscopy?
The ability to distinguish between two points.
How does higher resolution affect the image?
It gives a sharper image.
What can electron microscopes reveal in more detail?
Internal structures of mitochondria and chloroplasts, and tiny structures like ribosomes and plasmids.
What is the formula for magnification?
Magnification = image size / real size.
In the magnification formula, what should the image size and real size have in common?
The same units.
If the units for image size and real size are different, what must you do?
Convert them to the same units first.
Calculate the image size if the real size is 50 um and the magnification is x 100.
Image size = 5000 um or 5 mm.
What is standard form useful for in microscopy?
Writing very big or small numbers in a manageable way.
How is the number of places the decimal point moves represented in standard form?
By a power of 10.
If a number is 0.0025, what is its standard form?
2.5 x 10^-3.
True or False: The first number in standard form must be greater than 10.
False.
What happens to the power of 10 if the decimal point moves to the left?
It becomes positive.
What happens to the power of 10 if the decimal point moves to the right?
It becomes negative.
