Flashcards in Neuronanatomy Deck (20):
What is immunohistochemistry?
Manufacturing of antibodies for the protein required and visualising the antibodies in sections of brain tissues- it allows discrete labelling of cells
What can immunohistochemistry be combined with and why would this be done?
Fluorescence, to allow for multiple proteins to be stained in a single section of a tissue
What method uses a stream of electrons accelerated towards a specimen using a positive electrode, to detect and transform an image?
Transmission electron microscope
Why is electron microscopy required to view neurones?
As a light microscope is limited to 0.1 um and synapses are 0.02 um therefore an electron microscope is needed to view individual cells
How is ado plasmid transport been exploited?
Using horseradish peroxide which is retrogradely transported to reveal pathways in the brain.
What did electron microscopy reveal?
The existence of synapses and the ultra structure of neurones
What did Cajals neurone doctrine state?
That 'neurones are like other cells in the body and are the elementary unit of the brain'
What is retrograde transport?
From the axon terminals to the soma
What is anterograde transport?
From the soma to the axon terminals
What is axoplasmic transport?
The movement of substances along neurones via microtubles
What are axons bringing info form the somatic receptors to the brain?
They feed into the spinal cord via a spinal nerve and the dorsal root.
What are axons taking info from the CNS to the peripheral structures?
Leave via the spinal cord via a ventral root and a spinal nerve
What is NISSl stain used to detect and what does it stain?
Detects neutrons and glia in the brain and stains nuclei and rough ER
How is brain tissue prepared for light microscopy?
Thin slices are made after tissue is fixed with formaldehyde
What method of staining detects neurones in the brain and stain cell body neurites?
Name the four staining methods?
NISSL, DAPI, Golgi, nauta silver staining
What is DAPI used to detect?
DNA in living and fixed tissues
What method of staining is used to detect degenerating axons?
Nauta silver staining
What is used to make slices of tissue?