Neuronanatomy Flashcards Preview

Biology of The Mind > Neuronanatomy > Flashcards

Flashcards in Neuronanatomy Deck (20):
0

What is immunohistochemistry?

Manufacturing of antibodies for the protein required and visualising the antibodies in sections of brain tissues- it allows discrete labelling of cells

1

What can immunohistochemistry be combined with and why would this be done?

Fluorescence, to allow for multiple proteins to be stained in a single section of a tissue

2

What method uses a stream of electrons accelerated towards a specimen using a positive electrode, to detect and transform an image?

Transmission electron microscope

3

Why is electron microscopy required to view neurones?

As a light microscope is limited to 0.1 um and synapses are 0.02 um therefore an electron microscope is needed to view individual cells

4

How is ado plasmid transport been exploited?

Using horseradish peroxide which is retrogradely transported to reveal pathways in the brain.

5

What did electron microscopy reveal?

The existence of synapses and the ultra structure of neurones

6

What did Cajals neurone doctrine state?

That 'neurones are like other cells in the body and are the elementary unit of the brain'

7

What is retrograde transport?

From the axon terminals to the soma

8

What is anterograde transport?

From the soma to the axon terminals

9

What is axoplasmic transport?

The movement of substances along neurones via microtubles

10

What are axons bringing info form the somatic receptors to the brain?

Primary afferents.

They feed into the spinal cord via a spinal nerve and the dorsal root.

11

What are axons taking info from the CNS to the peripheral structures?

Motor efferents.

Leave via the spinal cord via a ventral root and a spinal nerve

12

What is NISSl stain used to detect and what does it stain?

Detects neutrons and glia in the brain and stains nuclei and rough ER

13

How is brain tissue prepared for light microscopy?

Thin slices are made after tissue is fixed with formaldehyde

14

What method of staining detects neurones in the brain and stain cell body neurites?

Golgi

15

Name the four staining methods?

NISSL, DAPI, Golgi, nauta silver staining

16

What is DAPI used to detect?

DNA in living and fixed tissues

17

What method of staining is used to detect degenerating axons?

Nauta silver staining

18

What is used to make slices of tissue?

A microtome

19

What is transneuronal tracing?

When there is one or more synapse, two labels can be used to detect both laters by colour.