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Flashcards in Principles of Micro 5 Deck (37)
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1

importance of mannitol salt agar

-has pH indicator so will change color if the bacteria is producing acids
-selective: will grow A and B but not C
-differential: A will look different from B

2

when is sabouraud's agar used

for fungi

3

advantage of sabouraud's agar

-antibiotics can be added to inhibit growth of bacteria
-pH can be adjusted

4

basic formulation of sabouraud's agar

per liter of medium:
10g peptone
40g glucose
15g Agar

5

Not all microorganisms can be grown on an agar plate so what are the exceptions

rickettsiaeceae and chlamydiaceae

6

unique features of rickettsiaeceae (genera: rickettsia, orientia)

-obligate intracellular (need host to grow so cannot grow in an agar -- need cell culture, embryonated eggs, animals)

-get ATP, AA, and metabolites from host

-zoonotic so associated with arthropod vector

7

what are the two types of zoonotic features of rickettsiaeceae (genera: rickettsia, orientia)

-R. prowazekii: transmitted via human body louse

-R. rickettsiae: transmitted via wood tick

8

other features of rickettsiaeceae (genera: rickettsia, orientia)

-small
-gram negative; don't stain well
-LPS weakly toxigenic
-replicate in cell cytoplasm

9

stain used in rickettsiaeceae (genera: rickettsia, orientia)

giemsa or gimenez stains

10

two genera of chlamydiaceae

chlamydia (c. trachomatis)
chlamydiophilia (c. pneumoniae, C. psittaci)

11

features of chlamydiaceae

small varying in size
coccoidal
non motile

12

ecological groups of chlamydiaceae

-human infection
-zoonotic infection (birds --> human)

13

examples of human and zoonotic infection in chlamydiaceae

human: trachoma, lymphogranuloma venereum
zoonotic: psittacosis/ornithosis

14

unique features of chlamydiaceae

-obligatory intracellular
-depend on host for ATP and NAD
-no peptidoglycan
-genus specific LPS

15

chlamydiaceae has life cycles with what two developmental forms

elementary bodies (EB)
reticulate bodies (RB)

16

what can't be synthesized and detected in chlamydiaceae

can't synthesize ATP or reoxidize NAD
can't detect flavoproteins or cytochromes

17

what is the architecture of fungi

-has chitin
-true nucleus, nuclear envelope, pores
-phospholipid bilayer sterol e.g. ergosterol
-mitochondria and other subcellular organells

18

why won't antibacterial that target bacteria cell wall affect fungi

fungi and bacteria cell wall are made of two different things. fungi's cell wall is made of chitin while bacteria's cell wall is made of peptidoglycan

19

why is fungi considered a saprophyte

it absorbs organic compounds produced by breakdown of non living organic matters so dead or decaying plants or animals

20

what are two forms of fungi

macroform fungi
microform fungi

21

what are the two types of microform fungi

yeast
moulds/filamentous fungi

22

are there fungi that can switch form between yeast and moulds/filamentous fungi

yes they are depending on environment
and there are some fungi that are true moulds/filamentous fungi or true yeasts

23

cell type of yeast and its form of reproduction

unicellular
asexual reproduction

24

structure and form of reproduction for moulds/filamentous fungi

structure: long filamentous/tubular structure called hyphae

reproduction: sexual and asexual (produces spores)

25

what is the habitat and classification of moulds/filamentous fungi

habitat: aquatic and terrestrial
classification: based on divisions (similar to phyla) which is based on rRNA analysis

26

if genus is amanita for filamentous/moulds, what is the family, order, class, phylum/division, kingdom

family - amanitaceae
order - agaricales
class - hymenomycetes
phylum/division - basidiomycotes
kingdom - fungi

27

interwoven mat of hyphae

mycelium

28

what is a septate hyphae

barrier between the cells but not a blocking barrier

29

what is present in non septate hyphae

chitin in cell wall
n acetyl glucosamine polymer

30

what is the difference between fungal spores and bacterial spores

fungal spores are reproductive while bacterial spores are for survival