Quiz 3: Alkaline Phosphatase Flashcards Preview

Clinical Chemistry > Quiz 3: Alkaline Phosphatase > Flashcards

Flashcards in Quiz 3: Alkaline Phosphatase Deck (3)
Loading flashcards...
1

ALP functions in body, Isoenzyme/Isoform

Catalyzes the transphorylation of a wide variety of synthetic substrates (removes phosphate groups from ortho-phosphoric bonds).
Bone, liver, kidney, intestine and placenta major sources of ALP
Isoenzyme gene coded.
Isoform, altered form of enzyme due to posttranslational modification (glycation, siliac acid etc)

2

ALP Ranges, Interferences

RI for total: 30-90 IU/L serum
RI for bone fraction: 7 - 30 IU/L serum
Interferences: hemolysis (6xRBC), diet, and stability

3

ALP Measurement Methods

p-Nitrophenyl phosphate to p-Nitrophenol colorless to yellow (405nm)
ALP fractionated by electrophoresis, HLPC and heat stability